An acoustofluidic sputum liquefier

Huang, Po‐Hsun; Ren, Liqiang; Nama, Nitesh; Li, Sixing; Li, Peng; Yao, Xianglan; Cuento, Rosemarie A.; Wei, Cheng-Hsin; Chen, Yuchao; Xie, Yuliang; Nawaz, Ahmad; Alevy, Yael G.; Holtzman, Michael J.; McCoy, J. Philip; Levine, Stewart J.; Huang, Tony Jun

doi:10.1039/c5lc00539f

Cited by 57 publications

(49 citation statements)

References 43 publications

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“…The system consists of a piezoelectric transducer disk, which is bonded onto a thin glass slide (22 mm × 40 mm × 100 μm) using epoxy glue. Owing to several advantages of piezoelectric transducer disks including low cost, small size and relatively large power output, they have been integrated into microfluidic platforms to generate acoustic waves, which are used to induce oscillations in trapped gas bubbles, or sharp edge microstructures built on the microchannel walls . The thin glass slide is placed on top of a polydimethylsiloxane (PDMS) post (5 × 5 × 5 mm) to allow for maximum vibration.…”

Section: On‐chip Production Of Egain Microdroplets With Controllable mentioning

confidence: 99%

On‐Chip Production of Size‐Controllable Liquid Metal Microdroplets Using Acoustic Waves

Tang

Ayan

Nama

et al. 2016

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Micro- to nanosized droplets of liquid metals, such as eutectic gallium indium (EGaIn) and Galinstan, have been used for developing a variety of applications in flexible electronics, sensors, catalysts, and drug delivery systems. Currently used methods for producing micro- to nanosized droplets of such liquid metals possess one or several drawbacks, including the lack in ability to control the size of the produced droplets, mass produce droplets, produce smaller droplet sizes, and miniaturize the system. Here, a novel method is introduced using acoustic wave-induced forces for on-chip production of EGaIn liquid-metal microdroplets with controllable size. The size distribution of liquid metal microdroplets is tuned by controlling the interfacial tension of the metal using either electrochemistry or electrocapillarity in the acoustic field. The developed platform is then used for heavy metal ion detection utilizing the produced liquid metal microdroplets as the working electrode. It is also demonstrated that a significant enhancement of the sensing performance is achieved by introducing acoustic streaming during the electrochemical experiments. The demonstrated technique can be used for developing liquid-metal-based systems for a wide range of applications.

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Section: On‐chip Production Of Egain Microdroplets With Controllable mentioning

confidence: 99%

On‐Chip Production of Size‐Controllable Liquid Metal Microdroplets Using Acoustic Waves

Tang

Ayan

Nama

et al. 2016

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“…without a post-processing step that is susceptible to numerical aberration. To illustrate this point, we consider the oscillating sharp-edge device considered by Nama et al (2014, 2016) and by Huang et al (2013, 2014, 2015 a , b ). Figure 6( a ) shows the schematic of a typical sharp-edge-based acoustofluidic device consisting of protruded sharp edges along the channel walls in a periodic fashion.…”

Section: Numerical Resultsmentioning

confidence: 99%

Acoustic streaming: an arbitrary Lagrangian–Eulerian perspective

2017

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We analyse acoustic streaming flows using an arbitrary Lagrangian Eulerian (ALE) perspective. The formulation stems from an explicit separation of time scales resulting in two subproblems: a first-order problem, formulated in terms of the fluid displacement at the fast scale, and a second-order problem, formulated in terms of the Lagrangian flow velocity at the slow time scale. Following a rigorous time-averaging procedure, the second-order problem is shown to be intrinsically steady, and with exact boundary conditions at the oscillating walls. Also, as the second-order problem is solved directly for the Lagrangian velocity, the formulation does not need to employ the notion of Stokes drift, or any associated post-processing, thus facilitating a direct comparison with experiments. Because the first-order problem is formulated in terms of the displacement field, our formulation is directly applicable to more complex fluid–structure interaction problems in microacoustofluidic devices. After the formulation’s exposition, we present numerical results that illustrate the advantages of the formulation with respect to current approaches.

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“…In our future work, we will incorporate our recently developed acoustofluidic sputum liquefier to get rid of the off-chip sample preparation steps. 14 In addition, an on-chip flow cytometry unit 47–49 will be incorporated to realize an automated, fully integrated device for point-of-care diagnostics and personalized treatment of asthma and other pulmonary diseases. We envision that the realization of such platforms could have significant impact on diagnosis and therapeutics of asthma and other pulmonary diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Several microfluidic platforms for sputum liquefaction 14 and analysis 15–18 have already been demonstrated. However, the microfluidic transfer of inflammatory cells from liquefied sputum samples has not yet been demonstrated.…”

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confidence: 99%

Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples

Ren

Huang

et al. 2016

Anal. Chem.

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For sputum analysis, the transfer of inflammatory cells from liquefied sputum samples to a culture medium or buffer solution is a critical step because it removes the inflammatory cells from the presence of residual dithiothreitol (DTT), a reagent that reduces cell viability and interferes with further sputum analyses. In this work, we report an acoustofluidic platform for transferring inflammatory cells using standing surface acoustic waves (SSAW). In particular, we exploit the acoustic radiation force generated from a SSAW field to actively transfer inflammatory cells from a solution containing residual DTT to a buffer solution. The viability and integrity of the inflammatory cells are maintained during the acoustofluidic-based cell transfer process. Our acoustofluidic technique removes residual DTT generated in sputum liquefaction and facilitates immunophenotyping of major inflammatory cells from sputum samples. It enables cell transfer in a continuous flow, which aids the development of an automated, integrated system for on-chip sputum processing and analysis.

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An acoustofluidic sputum liquefier

Cited by 57 publications

References 43 publications

On‐Chip Production of Size‐Controllable Liquid Metal Microdroplets Using Acoustic Waves

On‐Chip Production of Size‐Controllable Liquid Metal Microdroplets Using Acoustic Waves

Acoustic streaming: an arbitrary Lagrangian–Eulerian perspective

Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples

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