An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma

Abstract

BackgroundESPN (Espin), an actin filament-binding protein, plays an important role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Recent few studies show that ESPN regulates metastasis and cell proliferation in melanoma. However, the significance of ESPN in other cancers such as esophageal squamous cell carcinoma (ESCC) remains largely unknown.MethodsImmunohistochemistry was performed in 169 patients with ESCC and correlated with clinicopathological features and survival. The functional role of ESPN in ESCC cells was determined by ESPN-mediated siRNA.ResultsUnivariate analyses showed that high ESPN expression was associated with inferior overall survival (P = 0.005) and disease-free survival (P = 0.035). High ESPN expression was an independent prognosticator in multivariate analysis for overall survival (P = 0.009, hazard ratio = 1.688) and disease-free survival (P = 0.049, hazard ratio = 1.451). The 5-year overall survival rates were 30% and 54% in patients with high and low expression of ESPN, respectively. Inhibition of endogenous ESPN in ESCC cells decreased ESCC growth by reducing cell proliferating rates.ConclusionsHigh ESPN expression is independently associated with poor prognosis in patients with ESCC and downregulation of ESPN inhibits ESCC cell growth. Our results suggest that ESPN may be a novel therapeutic target for patients with ESCC.

A data-adaptive methods in detecting exogenous methyltransferase accessible chromatin in human genome using nanopore sequencing

“…5C–F ). The utilization of alternative TSSs or TESs imply the elongation of transcripts, such as RCC2, an oncogene associated with the proliferation ( Wu et al 2022 ), invasion ( Chen et al 2020 ), and epithelial-mesenchymal transition ( Pang et al 2017 ) of multiple cancers, or the truncation of transcripts, such as ESPN, a gene associated with cancer cell growth ( Li et al 2018 ). These phenomena indicated that the accessible Alu element affects the initiation and termination of nearby genes while the sequence itself does not necessarily transcribed.…”

Asciminib, a novel allosteric inhibitor of BCR‐ABL1, shows synergistic effects when used in combination with imatinib with or without drug resistance

“…These genes are associated with cancer growth. 30 , 31 , 32 , 33 However, there are no related reports on asciminib, imatinib, or CML, which may be novel sites of action that show synergistic effects.…”

Differential methylation of G-protein coupled receptor signaling genes in gastrointestinal neuroendocrine tumors

“…Chromosomal instability in tumors is promoted by DNA hypomethylation and is observed in multiple tumor types including pancreatic NETs 35 , 36 . The lncRNA, which made up 22.8% of the differentially methylated genes, has been shown to regulate cancer genes and represent additional disease pathways 37 – 40 . We examined the influence of tobacco use on methylation in GINET tumors.…”