An Actinophage for Streptomyces griseus

Streptomyces griseus (Krainsky) Waksman and Henrici

“…(6) Different strains of S. griseus vary greatly in their sensitivity to actinophage. The phage active against streptomycin-producing strains of S. griseus is not active against any other strains of this organism (Reilly, Harris, and Waksman, 1947).…”

Observations on the Chemical Inhibition of Streptomyces Griseus Bacteriophage Multiplication

“…This limits the use of sodium citrate or sodium oxalate as bacteriophage inhibitors to media that do not contain large amounts of these metallic ions; and of the media used in the production of streptomycin, the soybean meal glucose medium is singularly low in its metallic ion content. Such media components as cornsteep water, hydrolyzed rabbit fur, or meat hydrolyzates and extracts (Bennett, 1946;Woodruff et al, 1947;Reilly et al, 1947) are heavily contaminated with metallic ions, and the application of this method of inhibiting bacteriophage multiplication to fermentations in which they are included would require prohibitively large amounts of sodium citrate.…”

“…The methods used in the following experiments resembled in a general way those used by other investigators (Reilly et al, 1947;Koerber et al, 1950). Bacteriophage counts were made by adding quantities of the bacteriophagecontaining solutions (diluted in peptone solution-Koerber et at., 1950) to a 15-cm petri dish together with 0.1 to 0.3 ml of spores obtained from 5-to 7-day submerged culture of a bacteriophage-susceptible culture of S. griseus and mixing well with 10 ml of sterile liquefied Difco yeast beef agar (a yeast extract, meat extract, glucose agar).…”

The Actinomycetes-Their Nature, Occurrence, Activities and Importance.

“…The following method can be employed (355) for assaying the activity concentration of phage in a gi\'en preparation. A 3 to 5-dayold shaken culture of a streptomycin-producing strain of S. griseus is filtered aseptically through paper and inoculated on plates.…”