An Action of 5‐hydroxytryptamine on Adrenaline Receptors

Innes, I. R.

doi:10.1111/j.1476-5381.1962.tb01447.x

Cited by 60 publications

(34 citation statements)

References 13 publications

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“…All washes (indicated by W in the protocol) were by exchange of bath contents for fresh Krebs solution. Benextramine tetrahydrochloride (BHC) was included in order to avoid possible confounding of 5-HT responses due to direct or indirect a-adrenoreceptor stimulation by the agonist (Innes, 1962;Fozard & Mwaluko, 1976;Apperley et al, 1976;Marin et al, 1981). The sighting dose of 10 mM 5-HT was used only to establish tissue viability.…”

Section: Experimental Protocolsmentioning

confidence: 99%

An operational model of pharmacological agonism: the effect of E/[A] curve shape on agonist dissociation constant estimation

Black

Leff

Shankley

et al. 2010

British J Pharmacology

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1 An operational model of pharmacological agonism has been analysed to predict the behaviour of rectangular hyperbolic and non-hyperbolic agonist-concentration effect, E/[A], curves with variation in receptor concentration, [Ro]. 2 Irreversible antagonism is predicted to cause E/[A] curve gradient changes in non-hyperbolic cases but not in hyperbolic cases; in both cases estimation of agonist dissociation constants (KAs) is theoretically valid.

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Section: Experimental Protocolsmentioning

confidence: 99%

An operational model of pharmacological agonism: the effect of E/[A] curve shape on agonist dissociation constant estimation

Black

Leff

Shankley

et al. 2010

British J Pharmacology

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show abstract

“…All washes (indicated by W in the protocol) were by exchange of bath contents for fresh Krebs solution. Benextramine tetrahydrochloride (BHC) was included in order to avoid possible confounding of 5-HT responses due to direct or indirect a-adrenoreceptor stimulation by the agonist (Innes, 1962;Fozard & Mwaluko, 1976;Apperley et al, 1976;Marin et al, 1981). The sighting dose of 10 ,uM 5-HT was used only to establish tissue viability.…”

Section: Experimental Protocolsmentioning

confidence: 99%

An operational model of pharmacological agonism: the effect of E/[A] curve shape on agonist dissociation constant estimation

Black¹,

Leff²,

Shankley³

et al. 1985

British J Pharmacology

277

196

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“…The production of behavioural and electrocortical sleep by amines with substituted phenyl or indolyl ring structures suggested that they acted through similar mechanisms. Receptors common to adrenaline and 5-hydroxytryptamine occur in a number of systems (Innes, 1962).…”

Section: Adultfowlmentioning

confidence: 99%

Action of Sympathomimetic and Allied Amines on the Central Nervous System of the Chicken

Dewhurst¹,

Marley²

1965

British Journal of Pharmacology and Chemotherapy

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The effects of the a-methyl derivatives of noradrenaline, phenethylamine and tryptamine on the central nervous system of the chicken have been described (Dewhurst & Marley, 1965a). In the present experiments a larger series of amines and of their amino acid precursors has been tested on the central nervous system and on blood pressure. In addition, factors potentiating activity such as monoamine oxidase inhibition or diminishing activity such as tachyphylaxis or pharmacological antagonism have been studied.Preliminary accounts of this work have been presented (Dewhurst & Marley, 1964, and communication to the British Pharmacological Society, January, 1964). METHODSThe methods used for implanting electrodes and cannulae and for measuring cheeping and electrocortical and electromyographic activity were the same as those described previously (Dewhurst & Marley, 1965a,b). In addition to 1-to 28-day-old chickens, fowls up to 1 year old were used.To record blood pressure in the conscious unrestrained chicken a polyethylene cannula sealed with a nylon spigot and containing heparin-saline (100 mg/ml.) was tied into the upper part of a carotid artery; the tubing was brought through the scalp incision and fixed to the skull and wound into the strand of electrodes and the venous cannula. In the experiments in which electrocortical activity and blood pressure were recorded, the cortical electrodes were implanted on the left cerebral hemisphere and the blood pressure cannula was tied into the right carotid artery. At the time of the experiment, the spigot was removed and the arterial catheter was connected via saline-filled polyethylene tubing to a tap unit and screening micrometer transducer. The signals were amplified and displayed on a D.E. potentiometric recorder (Cambridge Instruments).Other blood pressure experiments were made with spinal chickens. These were prepared with halothane and oxygen anaesthesia given by endotracheal tube. After destroying the brain through an occipital craniotomy, the animal was artificially ventilated with a Palmer Miniature Ideal respiration pump. Excess air was allowed to escape through an adjustable leak in the circuit and round the endotracheal cannula. Blood pressure was recorded with a transducer connected to a cannula in the carotid or the ischiadic artery.Chromatography was carried out with the apparatus and techniques devised by Smith (1960). Amines were applied as the salts (detailed below) dissolved in 50% methanol in water to give an amine concentration of 10 pmoles/ml. Spots (1 id.) were applied with a Hamilton syringe. The major component of the developing solvent was oleyl alcohol (B.D.H.) which had been washed successively with equal volumes of N-sodium hydroxide, de-ionized water, N-hydrochloric acid, further water until washings were neutral and then shaken with an equal volume of 0.1 N-ammonifm acetate buffer, pH 7.4, for 20 min. The two

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An Action of 5‐hydroxytryptamine on Adrenaline Receptors

Cited by 60 publications

References 13 publications

An operational model of pharmacological agonism: the effect of E/[A] curve shape on agonist dissociation constant estimation

An operational model of pharmacological agonism: the effect of E/[A] curve shape on agonist dissociation constant estimation

An operational model of pharmacological agonism: the effect of E/[A] curve shape on agonist dissociation constant estimation

Action of Sympathomimetic and Allied Amines on the Central Nervous System of the Chicken

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