An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNA

Darnell, James; Wall, Randolph; Tushinski, Robert

doi:10.1073/pnas.68.6.1321

Cited by 367 publications

(116 citation statements)

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“…A poly(A) polymerase activity was first isolated from calf thymus in 1960 [5], and its role in pre-mRNA 3′-end processing was recognized ten years later [6][7][8]. Subsequent studies showed that the primary RNA transcripts extended beyond the site of polyadenylation, indicating that eukaryotic pre-mRNA 3′-end processing requires both cleavage and polyadenylation [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Protein factors in pre-mRNA 3′-end processing

Mandel

Bai

Tong

2007

Cell. Mol. Life Sci.

364

482

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Most eukaryotic mRNA precursors (pre-mRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3′-end. Processing at the 3′-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity of the processing reactions, more than 14 proteins have been identified for the mammalian complex, and more than 20 proteins have been identified for the yeast complex. The 3′-end processing machinery also has important roles in transcription and splicing. The mammalian machinery contains several sub-complexes, including cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factor I (CF I m ), and cleavage factor II (CF II m ). Additional protein factors include poly(A) polymerase (PAP), poly(A) binding protein (PABP), symplekin, and the C-terminal domain (CTD) of RNA polymerase II largest subunit. The yeast machinery includes cleavage factor IA (CF IA), cleavage factor IB (CF IB), and cleavage and polyadenylation factor (CPF).

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Section: Introductionmentioning

confidence: 99%

Protein factors in pre-mRNA 3′-end processing

Mandel

Bai

Tong

2007

Cell. Mol. Life Sci.

364

482

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“…Polyadenylic Acid Content of the in vitro Synthesized RNA. Most of the eukaryotic messenger RNAs and some of the HnRNA contain poly(A) sequences at their 3' end (21) (7) (8) (9). Marzluff et al (11) have shown that nuclei isolated from mouse myeloma cells are capable of synthesizing poly(A) sequences.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the eukaryotic messenger RNAs and some heterogeneous nuclear RNAs contain polyadenylic acid sequences (7,8,9).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of messenger RNA-like molecules in isolated myeloma nuclei

Mory

Getter

1977

Nucl Acids Res

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Nuclei isolated from mouse myeloma cells grown in tissue culture are capable of synthesizing RNA for prolonged periods of time. Addition of cytoplasmic extracts to the system stimulates slightly the rate and prolongs the time of synthesis.As judged by sedimentation in SDS and in formamide gradients, the size of the RNA synthesized is heterogeneous from smaller than 10S to larger than 45S, thus resembling in vivo made RNA.

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“…Terminal nucleotide sequences of mammalian messenger RNAs have become of particular interest recently due to the finding that untranslated polyadenylic acid linked to the messenger might play a role in the processing of nuclear precursor RNA (1)(2)(3)(4)(5). During infection of suitable cells, the single-stranded RNA of poliovirus serves as template in viral RNA replication and also as mRNA for virus-specific protein synthesis (6).…”

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confidence: 99%