In this study, the effects of open innovation practices on enterprise value were discussed based on the supply chain perspective. A nonlinear programming mode was constructed considering the uncertainty of open innovation effect. On this basis, the prototype was analyzed using a robust optimization approach with comprehensive considerations to the infeasible probability of constraint and goal accuracy. Findings show that parameter could convert the nonlinear programming model with uncertainty into a robust model with a strong stability. Furthermore, could be used to regulate the preference of the manufacturer to profit and the uncertainties in innovative practices. A small indicates manufacturers can refer to more information, and the manufacturers are more inclined to a mass production, so the profit is larger. Meanwhile, a high means that manufacturers can refer to less information, so that they pay more attention to making the product output adapt to the uncertainty of innovative effect. Manufacturers could select the appropriate value and reasonably arrange the production outputs of different goods according to the technological level of open innovation subject and their preference to profit and uncertainty. These conclusions were verified by a case study. In this work, the uncertainty of the open innovation effect was investigated from the supply chain perspective, which is of important significance to the decision making on optimal B Qingchun Meng