An advantageous method for detection of Fc-receptors and for studying Fc-receptor-mediated phagocytosis

Fornůsek, L; Kopeček, Jindřich; Větvička, Václav

doi:10.1016/0165-2478(83)90051-2

Cited by 15 publications

(5 citation statements)

References 10 publications

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“…Fluoresceinated HEMA (HEMA-FITC) methacrylate copolymer beads [32] were resuspended in culture medium (see above) and incubated in chambers for 3 h at 37°C. After extensive washing (5-7 times), a coverslip was placed over the live cells and they were examined immediately under a Zeiss Photomicroscope 111.…”

Section: Phagocytosismentioning

confidence: 99%

Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors

1988

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Stromal cells which grow as an adherent layer of Whitlock-Witte cultures are thought to be an essential component of the lymphohemopoietic microenvironment. Stromal cell lines from bone marrow (BM) and spleen have been obtained by treatment of cultures with 5-fluorouracil and selected for their lymphocyte support capacity by measuring the clonal growth of stromal cell-dependent lymphocyte lines in methyl cellulose. Established stromal cell lines differed significantly from stromal cells in primary Whitlock-Witte cultures with respect to expression of certain hemopoietic cell surface markers. For example, the Thy-1 and Mac-3 antigens were expressed by stromal cell lines obtained from BM and spleen, but not by stromal cells in primary cultures. Features common to all stromal cells include synthesis of actins, the neural adhesion molecule N-CAM, and a variety of collagens. Two types of common leukocyte antigens were not significantly expressed. The proliferation and total protein synthetic capacity of lymphocyte-supportive stromal cell lines was sensitive to ionizing radiation. After exposure of the cells to 200 rads, the incorporation of either [3H]thymidine or [3H]Leucine was reduced to less than 50% of control values, but the growth of lymphocytes was augmented in the presence of an irradiated stromal cell layer. The proliferation of stromal cell lines was also affected by exposure to a variety of growth factors. Addition of epidermal growth factor or endothelial cell growth factor augmented BM or spleen-derived stromal cell proliferation, while interferon-gamma had the opposite effect. In general, but not exclusively, lymphocyte growth was inhibited by factors which augmented the proliferation of stromal cells. Novel methods are described for isolating stromal cells and determining their capacity to support lymphocyte growth in vitro. Evidence is presented that this ability is not restricted to BM-derived stromal cells. The function of stromal cells was not dependent on their ability to proliferate, and this may be modulated by immunoregulatory and other growth factors.

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Section: Phagocytosismentioning

confidence: 99%

Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors

1988

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“…Staining was performed by the May-Grünwald Giemsa-Romanowski method. Cells were considered positive (normal phagocyting activity) when they phagocytized three or more particles (8).…”

Section: Methodsmentioning

confidence: 99%

Exposure to nanoparticles of magnetite Fe<sub>3</sub>O<sub>4</sub> in three different doses and their influence on selected respiratory parameters of bronchoalveolar lavage after intravenous instillation

Hurbánková,

Volkovová,

Černá

et al. 2023

Cent Eur J Public Health

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Objectives: Due to nano-dimensions (less than 100 nm), can nanoparticles probably penetrate through various membranes and travel from the bloodstream to other organs in the body. The aim of our study was to find out whether NPs Fe 3 O 4 (coated with sodium oleate) injected into the tail vein of laboratory Wistar rats pass through the bloodstream to the respiratory tract (in comparison with a control group); and if so whether increasing doses of NPs Fe 3 O 4 have an escalating harmful effect on selected bronchoalveolar lavage (BAL) parameters.Methods: Wistar rats were intravenously given 3 doses of the suspension of NPs Fe 3 O 4 (0.1% LD50 = 0.0364, 1.0% = 0.364 and 10.0% = 3.64 mg/kg animal body weight). Seven days later, we sacrificed the animals under anaesthesia, performed bronchoalveolar lavage (BAL), and isolated the collected cells. Many inflammatory and cytotoxic BAL parameters were examined.Results: Both inflammatory and cytotoxic BAL parameters affected by Fe 3 O 4 suspension were changed compared to control results, but not all were statistically significant. Thus, the NPs Fe 3 O 4 passed through the bloodstream to the respiratory tract and affected it. The highest concentration of NPs Fe 3 O 4 (10%) had the most influence on BAL parameters (7 of 12 parameters). Only 3 parameters showed a pure dose dependence.Conclusion: We assume that the adverse effect of Fe 3 O 4 NPs in our study is probably not correlated with the dose, but rather with the size of the particles or with their surface area.

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“…A traditional EA-rosette assay with antibody-coated TNP-SRBC was used (2 I). SRBC coupled with the trinitrophenyl hapten (19) were coated by the maximal subagglutinating doses of monoclonal anti-TNP antibodies of different immunoglobulin isotypes (7). Briefly, lo6 cells were mixed with 0.5 ml of 1 9'0 suspension of antibody-coated TNP-SRBC, centrifuged (120 G, 5 min) and incubated for 60 rnin at 22 "C. Iodoacetic acid (Sigma, St. Louis, MO) was added to the cell suspension in order to block FcR-mediated phagocytosis (2).…”

Section: Cellsmentioning

confidence: 99%

Effects of bilirubin on murine peritoneal and spleen cells

Vetvickka

Fornůsek

Šíma

et al. 1988

APMIS

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and 4Re~earch Institute for the Care of Mother and Child, Prague, Czechoslovakia WtviEka, V., Fornusek, L., Sima, P., Bilej, M., Taborsky, L., kihova, B., Sirneckova, J., Miler, I. Effects of bilirubin on murine peritoneal and spleen cells. APMIS 96: 671-675, 1988.The effect of bilirubin on murine peritoneal and spleen cells was investigated. Bilirubin was found to have a strong and rapid effect on the expression of various kinds of Fc receptors on peritoneal macrophages. Significant changes were observed 30 min after the infection of bilirubin. The return to normal values was not observed earlier than after 24 h. The effect of bilirubin on Fc receptor expression of splenic macrophages was less pronounced. Expression of Ia antigen on macrophages was not influenced by bilirubin. The changes in percentage of sIg' and Thy 1.2' lymphocytes reflect a change in the ratio of T to B cells in the peritoneal cavity, as bilirubin caused 40% increase in numbers of B cells and a similar decrease in numbers of T cells. The percentage of splenic B lymphocytes was not influenced by bilirubin injection; but the ratio of T helper to suppressor cells was altered.receptor expression and phagocytic activity of mouse peritoneal marophages. Folia Microbiol. 30: 25. Yunemasu, K.: Anti-human Clq: Rapid and simple method for preparing monospecific antisera.

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An advantageous method for detection of Fc-receptors and for studying Fc-receptor-mediated phagocytosis

Cited by 15 publications

References 10 publications

Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors

Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors

Exposure to nanoparticles of magnetite Fe<sub>3</sub>O<sub>4</sub> in three different doses and their influence on selected respiratory parameters of bronchoalveolar lavage after intravenous instillation

Effects of bilirubin on murine peritoneal and spleen cells

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