An agglutinogen common to certain strains of lactose and non-lactose-fermenting coliform bacilli

Stamp, Lord; Stone, Doris M.

doi:10.1017/s002217240001295x

Cited by 34 publications

(15 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fimbrial antigens bear important resemblances to some of the known crossreacting antigens of enterobacteriaceae, in particular the X-antigen described in salmonellae by Topley & Ayrton (1924), Happold (1928, 1929) and Cruickshank (1939, the a-antigen of Stamp & Stone (1944) and the ,-antigen of Mushin (1949). The x-antigen, however, differs in not reacting with natural agglutinins in normal human sera, while the ,8-antigen occurs in some species never yet found fimbriate (e.g.…”

mentioning

confidence: 89%

The fimbrial antigens ofShigella flexneri

Gillies

Duguid

1958

J. Hyg.

View full text Add to dashboard Cite

1. ‘Pure fimbrial antisera’ were prepared for Sh. flexneri strains of O-serotypes 1a, 2b, 3 and 4a and 5, by injecting rabbits with a living fimbriate-phase culture and absorbing the crude immune serum with a non-fimbriate-phase culture of the same strain to remove antibodies for the non-fimbrial (somatic) antigens. These sera gave at high titre a rapid, loosely floccular agglutination of all fimbriate-phase Flexneri cultures, caused adhesion of their fimbriae visible by electron-microscopy and inhibited their haemagglutinating activity. ‘Non-fimbrial antisera’, prepared by injection of non-fimbriate-phase cultures, were devoid of these activities; they gave somatic-type agglutination with fimbriate bacilli at lower titres than with homologous non-fimbriate bacilli, the fimbriae tending to mask the O-antigens and confer relative O-inagglutinability.2. Heating at 90°C. detached the fimbriae from the bacilli, so that these lost their haemagglutinating activity, fimbrial serum agglutinability and fimbrial agglutinin-binding power. When heated for 21/2 hr. at 100°C., the detached fimbriae retained agglutinin-binding power, but lost their immunogenicity.3. Cross-agglutination and absorption tests showed that the antigenic composition of the fimbriae was identical in all flexneri strains, irrespective of O-serotype. The flexneri pure fimbrial antisera agglutinated at low titre sixty out of sixty-six fimbriate Bact. coli strains. Absorption tests with a flexneri and three coli pure fimbrial antisera showed that the fimbriae of Sh. flexneri contained a major ‘flexneri-specific antigen’, found in only one coli strain, one or more minor ‘flexneri-coli antigens’ shared by a few coli strains. The coli fimbriae also contained a major, ‘coli type-specific antigen’ shared in groups of several related strains. The flexneri sera did not react with fimbriate strains of Bact. cloacae, Salmonella and Proteus.4. Fimbrial agglutinins for Sh. flexneri were found in eighty of eighty-one normal human sera at titres from 30 to 1920, and in some pre-immunization rabbit sera at titres of 30 to 60.

show abstract

mentioning

confidence: 89%

The fimbrial antigens ofShigella flexneri

Gillies

Duguid

1958

J. Hyg.

View full text Add to dashboard Cite

show abstract

“…Stuart, Wheeler & McGann (1946) studied this group in more detail and described six biochemical subgroups which differed slightly in their fermentation reactions with carbohydrates. They included in this group certain other strains which had previously been described in the literature as Shigella make$eld (Berger, 1945) and the Sachs types B 81 and B 105 described as mannitol negative types of Shigella (Sachs, 1943) and it was suggested that type 29911 should be classified as a transitional group without specific or generic status.…”

Section: Discussionmentioning

confidence: 99%

“…When Proteus morganii, strain 1721, of Stamp & Stone (1944) was examined, it was found to produce amines but did not decompose urea or require nicotinic acid as an essential metabolite. It was not therefore a typical Proteus species, and Kauffmann (1951) placed this strain in the Providence group.…”

mentioning

confidence: 99%

Amine Production and Nutrition in the Providence Group

Proom

1955

Journal of General Microbiology

View full text Add to dashboard Cite

“…It is possible that the non-specific component reacts with similar components in other bacterial species, since Proteus strains have been described which have receptors in common with Salmonella strains (Maccolini, 1940 The variation A -+ B shows most of the characters of a partial S --f R variation. It was possible, in spite of the fact that all suspensions used were treated with ethanol, that the a-antigen (Stamp & Stone, 1944) may play some part; but all five of the B suspensions failed to agglutinate with an anti-a serum, and it is safe to say that in these cases the phase B antigen complex is not homologous in any of its parts with the a-antigen. …”

Section: Discussionmentioning

confidence: 99%

Cultural and Serological Phases of Proteus vulgaris

Belyavin

1951

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARY: Three phases of Proteus vulgaris, A, B and C, are distinguishable by cellular and colonial morphology, and to some extent serologically. Phase A is the modal form of freshly-isolated strains ; it has a uniform bacillary morphology, swarms intermittently on nutrient agar, and forms stable suspensions in 0.85 yo saline.Phase B strains, though possessing flagella, are usually non-motile, non-swarming, and highly pleomorphic; usually form unstable suspensions. Phase C strains are uniformly filamentous, motile, often swarm in a continuous film, and stability in saline varies with the strain.The somatic surface of phase A strains is characterized by a dominant typespecific antigen, and traces of a non-specific and a possibly strain-specific antigen. In phase B strains the type-specific antigen is largely lost, and the other two antigens dominate the surface. The antigenic surface of phase C strains does not differ markedly from that of phase A.The phase variations A-tB and A+C are reversible. Phase B resembles a partially R form, in which the mouse-virulence is significantly less than that of the parent phase A strain.The first serological study of colonial variation in Proteus vulgaris is that of Weilk Felix (1916,1917) in their classical paper describing the 0 and H variants. This was amplified later by Weil (1920, 1923) and Felix (1923) who described variations of colonial morphology in the 0 strains of P. vulgaris. Both authors described a modal colony, and one which was opaque grew more slowly than the modal on nutrient agar at room temperature, and gave a finely granular growth in broth. In addition, Felix described a non-swarming (0) colony which had an irregular or ill-defined edge, and was granular ('griessig'). Although the small-colony variant and the apparently 'normal' type of colony to which it occasionally reverted, differed serologically from the parent ' normal ' strain, no association was demonstrated between changes in antigenic constitution and colonial form. Both authors regarded this variation as a specific kind of group transformation, largely because Weil found that his small-colony variant of an X 19 strain had developed an antigenic receptor identical with that found in his X 2 strains. Felix confirmed this, and regarded many of his variant strains as intermediate stages (' ubergangsformen ') in this antigenic reconstruction.Few of the later workers on the morphology and serology of the Proteus group mention colonial or antigenic variation (Taylor, 1928 He described a granular colony with a rough surface and irregular edge, which formed an auto-agglutinable suspension in 0.85 yo saline and which he regarded

show abstract

An agglutinogen common to certain strains of lactose and non-lactose-fermenting coliform bacilli

Cited by 34 publications

References 9 publications

The fimbrial antigens ofShigella flexneri

The fimbrial antigens ofShigella flexneri

Amine Production and Nutrition in the Providence Group

Cultural and Serological Phases of Proteus vulgaris

Contact Info

Product

Resources

About