An Agrobacterium transformation in the evolution of the genus Nicotiana

Furner, Ian J.; Huffman, Gary; Amasino, Richard M.; Garfinkel, David; Gordon, Milton P.; Nester, Eugene W.

doi:10.1038/319422a0

Cited by 151 publications

(108 citation statements)

References 13 publications

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“…Soon after the discovery of Agrobacterium-mediated gene transfer, the fixation of A. rhizogenes T-DNAs in the Nicotiana genome was reported (21,22). These T-DNA sequences, originating from different A. rhizogenes strains, are present in Nicotiana spp., are transmitted to the progeny, and are expressed at low levels but are not associated with the hairy root or tumor-like symptoms that are usually induced by Agrobacterium infections (23)(24)(25).…”

Section: Significancementioning

confidence: 99%

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

Kyndt

Quispe

Zhai

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

248

161

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Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. While assembling small interfering RNAs, or siRNAs, of sweet potato plants for metagenomic analysis, sequences homologous to T-DNA sequences from Agrobacterium spp. were discovered. Simple and quantitative PCR, Southern blotting, genome walking, and bacterial artificial chromosome library screening and sequencing unambiguously demonstrated that two different T-DNA regions (IbT-DNA1 and IbT-DNA2) are present in the cultivated sweet potato (Ipomoea batatas [L.] Lam.) genome and that these foreign genes are expressed at detectable levels in different tissues of the sweet potato plant. IbT-DNA1 was found to contain four open reading frames (ORFs) homologous to the tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), and agrocinopine synthase (Acs) genes of Agrobacterium spp. IbT-DNA1 was detected in all 291 cultigens examined, but not in close wild relatives. IbT-DNA2 contained at least five ORFs with significant homology to the ORF14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ ORF17n genes of A. rhizogenes. IbT-DNA2 was detected in 45 of 217 genotypes that included both cultivated and wild species. Our finding, that sweet potato is naturally transgenic while being a widely and traditionally consumed food crop, could affect the current consumer distrust of the safety of transgenic food crops.horizontal gene transfer | Agrobacterium spp. | food safety | sweet potato | transgenic crops

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Section: Significancementioning

confidence: 99%

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

Kyndt

Quispe

Zhai

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

248

161

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“…Furner et al, 1986). According to Harding (1996) recent recombination between the genomes of tobacco and a geminivirus resulted in the integration of 1000 nucleotides of viral DNA into the tobacco genome, enough to encode for a protein.…”

Section:       mentioning

confidence: 99%

The release of genetically engineered micro‐organisms and viruses into the environment

Giddings

1998

New Phytologist

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This review considers the reasons for, and research governing, the regulation and monitoring of genetically engineered micro‐organisms and viruses (GEMs) released into the environment. The hazards associated with releasing GEMs into the environment are the creation and evolution of new pests and diseases, and damage to the ecosystem and non target species. The similarities and differences between GEMs and conventional micro‐organisms are discussed in relation to risk assessment. Other issues covered include the persistence of micro‐organisms in the environment, transgene dispersal to non‐engineered microbes and other organisms, the effects of transgenes and transformation on fitness, and the evolution of pests and pathogens that are given or acquire transgenes. Areas requiring further research are identified and recommendations for risk assessment made.

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“…Solanaceae) и Linaria (сeм. Plantaginaceae) последовательностей, гомологичных Т-ДНК Agrobacterium rhizogenes (White et al, 1983;Furner et al, 1986;Meyer et al, 1995;Frundt et al, 1998;Matveeva et al, 2012). Патогенная почвенная бак-терия Agrobacterium переносит и встраивает в геном растения Т-ДНК -фрагмент большой плазмиды Ti или Ri, вызывающей образование опухо-лей -корончатых галлов или «бородатых» корней соответственно.…”

unclassified

“…Интересно отметить, что дан-ные последовательности обнаружены у всех исследованных представителей указанных видов, вне зависимости от экологических и географических условий их произрастания. То есть наличие Т-ДНК в геноме является характеристикой вида (White et al, 1983;Furner et al, 1986;Meyer et al, 1995;Frundt et al, 1998;Intrieri, Buiatti, 2001).…”

unclassified

Investigations of possible horizontal gene transfer from Agrobacterium to some representatives of the family Solanaceae

Kulayeva

Алексеевна²,

Matveeva

et al. 2013

Ecological genetics

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The development of methods of genetic engineering of plants poses in modern society the question of the safety of transgenic plants. Transformation of plants has been criticized as artificial process performed only in the laboratory. At the same time there is evidence that some members of the genus Nicotiana and Linaria contain sequences homologous to the T-DNA of Agrobacterium rhizogenes in their genomes. The question: whether there are other examples of horizontal gene transfer from Agrobacterium to the plant among the representatives of the family. Solanaceae, is still actual. We performed screening of representatives of four genera of the family Solanaceae for the presence of sequences homologous to oncogeniclike genes rolB, rolC, ORF13, ORF14. New examples of horizontal gene transfer were not detected. Apparently, the presence of sequences homologous to oncogenes of A. rhizogenes in the genome of nontransformed plants is not common to the whole family Solanaceae and is characteristic only of the some species of the genus Nicotiana.

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An Agrobacterium transformation in the evolution of the genus Nicotiana

Cited by 151 publications

References 13 publications

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

The release of genetically engineered micro‐organisms and viruses into the environment

Investigations of possible horizontal gene transfer from Agrobacterium to some representatives of the family Solanaceae

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