An Alkaline Protease from Bacillus pumilus MP 27: Functional Analysis of Its Binding Model toward Its Applications As Detergent Additive

Baweja, Mehak; Tiwari, Rameshwar; Singh, Puneet Kumar; Nain, Lata; Shukla, Pratyoosh

doi:10.3389/fmicb.2016.01195

Cited by 82 publications

(43 citation statements)

References 52 publications

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“…Another study reported similar optimum pH for protease production (36). Furthermore, there were also reports that the best pH for protease production was 8.0(37), 9.0 (38), 10.0 (39), and 11.0 (38,40). The pH value affects the absorption of nutrients by affecting the degree of ionization of the nutrients, cell membrane charge and membrane permeability.…”

Section: Optimization Of Culture Conditions For Proteasementioning

confidence: 87%

Simultaneous Production of Β-glucanase and Protease From Bacillus Velezensis Strain Identified in the Manure of Piglets.

Khalid¹,

Miao²,

Wei³

et al. 2020

Preprint

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Background: β-glucanase and protease, are the representative of most worldwide enzyme market. In this study, a strain producing β-glucanase and protease, identified as one type of Bacillus velezensis and named Y1, was isolated from the manure of piglet.Results:Its optimal β-glucanase-producing conditions were as: fermentation temperature of 37 °C, fermentation time of 60 h, initial pH of 6.0, outfit fluid amount of 50 mL/250 mL and carbon to nitrogen ratio was 1:1. The properties of the β-glucanasewere that the most suitable reaction temperature was 65°C, the optimum pH was pH 6.0. The optimal protease-producing conditions were fermentation temperature of 37 °C, fermentation time of 60 h, initial pH of 7.0, outfit fluid amount of 75/250 mL and carbon to nitrogen ratio of 2:3.The properties of the protease showed that the most suitable reaction temperature was 50°C, the optimum pH was 6.0. And the enzyme had the best stability at 45°Cand pH 7.0. The effects of each factor on producing β-glucanase and protease by B. velezensis Y1 were as follows: temperature > time > pH > loaded liquid volume. The amplified PCR fragments of β-glucanase and protease were 1434 bp containing an open reading frame of 1413 bp encoding a protein with 444 amino acids and 1752 bp containing an open reading frame of 1521 bp encoding a protein with 506 amino acids, respectively.Conclusion: This study identified the enzymes production, optimized the fermentation conditions of β-glucanase and protease by B. velezensis. As well as the enzyme properties were investigated and genes were cloned.

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Section: Optimization Of Culture Conditions For Proteasementioning

confidence: 87%

Simultaneous Production of Β-glucanase and Protease From Bacillus Velezensis Strain Identified in the Manure of Piglets.

Khalid¹,

Miao²,

Wei³

et al. 2020

Preprint

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“…Hence, this alkaline protease can be used as an additive in commercial detergent, as it is also effective in removing stains in cold water washes. 48 Alkaline serine protease was isolated from Bacillus licheniformis NH1. The protease was active from pH 7-12 with an optimum pH of 10-11.…”

Section: Detergentsmentioning

confidence: 99%

A Review on Microbial Alkaline Protease: An Essential Tool for Various Industrial Approaches

Sharma

Gat

Arya

et al. 2019

Industrial Biotechnology

112

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Proteolytic enzymes are present in all living organisms and help in cell growth and differentiation. Proteases are the hydrolytic enzymes that act as biocatalysts for the cleavage of proteins into smaller peptides and amino acids. Microorganisms have turned out to be a competent and inexpensive source of alkaline protease enzymes that can produce a continuous and consistent supply of desired product. Alkaline proteases have extensive application in various industrial sectors especially in detergent and leather industries. However, their application in food has not been much exploited. This review summarizes all the reports of applications of alkaline protease in different sectors with a main view on food applications. The effect of various physiochemical parameters on alkaline protease is discussed. Different sources of isolation and optimum pH and temperature of alkaline protease producing bacterial and fungal species are also reported.

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“…All of these are well suited to detergent formulations particularly at alkaline pH (Sumantha et al 2006). Protease from Bacillus pumilus MP 27 was considered as a good candidate for detergent industry due to its broad substrate range, activity over high and low temperatures and pH, and compatibility with surfactants and commercial detergents (Baweja et al 2016). Bacillus subtilis KT004404, an isolate from hydrothermal vents, produced a metalloprotease that has high resistance towards anionic surfactants, solvents and detergents.…”

Section: Proteases In Detergent Industrymentioning

confidence: 99%

“…There are several reports of thermostable and wide range pH stable proteases from Bacillus spp. which have excellent compatibility for industrial processes (Baweja et al 2016;Guleria et al 2016). The genus Bacillus is represented by Gram-positive, spore-forming, rod-shape bacteria that are obligate aerobes or facultative anaerobes (Dijl and Hecker 2013).…”

Section: Introductionmentioning

confidence: 99%

Potential application spectrum of microbial proteases for clean and green industrial production

Singh

Bajaj

2017

Energ. Ecol. Environ.

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Enzymes are the cornerstones of metabolism and constitute the fundamental basis for existence of life. However, recently enzymes are being implicated in diverse industrial processes because of their specific and fast action for efficient bioconversion of substrate to product, and their capability to save raw materials, energy and chemicals for various manufacturing processes. Enzymes are considered as environment-friendly (green) chemicals that may potentially help replacing completely or reducing the usage of hazardous chemicals for industrial processes, thus promising sustainable production and manufacturing. Among various industrial enzymes microbial proteases dominate the world enzyme market due to their multifaceted application potential in varied bioindustries like food, pharmaceutical, textile, photographic, leather and detergent. Promising applications of proteases in agricultural sector for instance may include biocontrol of pests, degumming of silk, selective delignification of hemp and wool processing. However, for successful industrial applications the proteases must be robust enough to suit the process conditions which are generally hostile. Proteases intended for industrial applications must have activity and stability over wide range of temperature and pH extremes for prolonged time periods and even in the presence of various potential enzyme inhibitors. Of various microbial proteases those from Bacillus spp. have got special significance because the latter are known for their ability to produce sturdy enzymes that might have suitability for industrial process conditions. The current article presents an interpretive summary of the recent developments on application potential of proteases for various industries.

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An Alkaline Protease from Bacillus pumilus MP 27: Functional Analysis of Its Binding Model toward Its Applications As Detergent Additive

Cited by 82 publications

References 52 publications

Simultaneous Production of Β-glucanase and Protease From Bacillus Velezensis Strain Identified in the Manure of Piglets.

Simultaneous Production of Β-glucanase and Protease From Bacillus Velezensis Strain Identified in the Manure of Piglets.

A Review on Microbial Alkaline Protease: An Essential Tool for Various Industrial Approaches

Potential application spectrum of microbial proteases for clean and green industrial production

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