An all-in-one enzymatic DNA network based on catalytic hairpin assembly for label-free and highly sensitive detection of APE1

Inhibition of non-small cell lung cancer metastasis by knocking down APE1 through regulating myeloid-derived suppressor cells-induced immune disorders

“…As a multifunctional protein, dysregulation of APE1 is associated with tumor occurrence, development, tumor angiogenesis, progression, and metastasis [ 23 ]. In relation to clinical pathological features, overexpression of APE1/Ref-1 is correlated with chemotherapy resistance, poor prognosis, and shorter survival periods [ 24 , 25 ]. This study revealed that patients with low levels of APE1 expression exhibit better overall survival, suggesting that high APE1 expression can serve as an adverse prognostic factor for tumor.…”

Enzyme Reaction-Assisted Programmable Transcriptional Switches for Bioactive Molecule Detection

“…33 These results clearly supported the proposed transcriptional switches, and we chose variant #4 in Figure 2k as the transcriptional switch for subsequent detection. To support that this strategy was applicable to various bioactive molecules, the above PAGE and fluorescence experiments were carried out for the apurinic/apyrimidinic endonuclease 1 (APE1) model 34 and Nb.BbvCI model 35 as well. By simply adjusting the sequence characteristics to adapt to enzyme cleavage reactions, switches were also successfully transcribed and showed excellent detection performance (Figure S4).…”