An Alternative Approach to the Traditional Mixotrophic Cultures of Haematococcus pluvialis Flotow (Chlorophyceae)

Göksan, Tolga

doi:10.4014/jmb.0909.09005

Cited by 43 publications

(19 citation statements)

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“…Ding et al [71] reported that under steady-state conditions, astaxanthin content in H. lacustris was 0.41%. The previous studies on the cultivation of H. lacustris have showed that acetate appears to be an important carbon source, enhancing both growth and carotenogenesis [45,72,73]. However, the effect of acetate was concentration dependent, higher concentrations inhibiting growth but markedly increasing astaxanthin content per cell [74].…”

Section: Discussionmentioning

confidence: 96%

“…lacustris is a photosynthetic green microalga that can produce astaxanthin in cells. In general, astaxanthin production from H. lacustris is accomplished through a twostage cultivation process including vegetative (green) and aplanospore (red) stages [44,45]. The accumulation of astaxanthin is affected by environmental stress factors such as light, temperature, pH, salt concentration and nutritional stresses [11].…”

Section: Discussionmentioning

confidence: 99%

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Influence of agriculture fertilizer for the enhanced growth and astaxanthin production from Haematococcus lacustris RRGK isolated from Himachal Pradesh, India

et al. 2019

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In the present study, Haematococcus lacustris RRGK which is the former name of H. lacustris HPI-001 isolated from Himachal Pradesh, India, and another strain H. lacustris SAG-19a retrieved from Gottingen Culture Collection, Germany, were used. The H. lacustris SAG-19a served as a control. The both strains were grown in a Bold basal medium in which the components of NaNO 3 , K 2 HPO 4 •3H 2 O and KH 2 PO 4 were replaced with agriculture fertilizers such as NPK (17:17:17), urea (CH 4 N 2 O) and DAP (diammonium phosphate; (NH 4 ) 2 HPO 4 + potash (K 2 CO 3 ) in different concentrations. The isolate of H. lacustris HPI-001 showed maximum growth at 1.2 mM NPK, while the H. lacustris SAG-19a showed maximum growth at 1.5 mM. However, both the strains showed maximum astaxanthin content at 0.6 mM NPK. The H. lacustris HPI-001 showed a maximum growth and astaxanthin content with 3.3 mM and 4.9 mM of urea, respectively, while H. lacustris SAG-19a showed maximum growth and astaxanthin accumulation with 3.3 mM and 6.6 mM of urea, respectively. The H. lacustris HPI-001 showed maximum growth at 250 mg/L (NH 4 ) 2 HPO 4 + 200 mg/L K 2 CO 3 and maximum astaxanthin content at 150 mg/L (NH 4 ) 2 HPO 4 + 100 mg/L K 2 CO 3 , while the maximum growth in H. lacustris SAG-19a was observed at 200 mg/L (NH 4 ) 2 HPO 4 + 150 mg/L K 2 CO 3 , and the maximum astaxanthin content at 150 mg/L (NH 4 ) 2 HPO 4 + 100 mg/L K 2 CO 3 . The basal medium was also amended with the addition of commercial NaHCO 3 wherein the maximum growth and content of astaxanthin in H. lacustris HPI-001 was at 0.6 mM and 1.5 mM of NaHCO 3 , respectively. The H. lacustris SAG-19a showed maximum growth and astaxanthin content at 1.5 mM and 0.6 mM, respectively. Based on the investigation, a medium was formulated and named as modified HPI-001 medium. The results of the present study suggest that commercial agricultural fertilizers may be used as excellent substitutes to enhance cell growth and astaxanthin production in H. lacustris.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Influence of agriculture fertilizer for the enhanced growth and astaxanthin production from Haematococcus lacustris RRGK isolated from Himachal Pradesh, India

et al. 2019

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“…Göksan et al (2010), ao adicionar acetato de sódio no meio de cultura de células de H. pluvialis no final da fase exponencial, obtiveram células encistadas (aplanósporos vermelhos) com maior peso seco e maior concentração de pigmentos, o que mostra que a adição de uma fonte de carbono é benéfica nessa etapa de indução à carotenogênese. Resultados semelhantes foram obtidos por Kobayashi et al (1993) Durante 15 dias, tempo necessário para a cultura atingir a fase exponencial, as células foram aclimatadas em frascos com capacidade de 1 L, que receberam 10.000 células mL -1 de H. pluvialis.…”

Section: Methodsunclassified

Carotenogênese em células de Haematococcus pluvialis induzidas pelos estresses luminoso e nutricional

Nunes

Vieira

Pinto

et al. 2013

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar as respostas das células de Haematococcus pluvialis ao processo de indução à carotenogênese, sob estresse luminoso e nutricional. As células foram aclimatadas durante 15 dias em meio WC, com aeração com ar atmosférico sintético filtrado e fluxo de 100 mL min ; e cultivo nas mesmas condições do tratamento anterior, mas com aeração contendo 4% de CO 2 . Os tratamentos foram conduzidos em triplicata, durante dez dias. Com a adição de CO 2 e o incremento da iluminação, observou-se aumento da razão carotenoides/clorofila e da biomassa celular. As células cessaram a divisão no segundo dia de estresse, quando o nitrato se tornou limitante, e aumentaram significativamente seu biovolume. A excreção de carbono orgânico e a concentração de astaxantina aumentam em resposta à adição de CO 2 . O estresse por intensidade luminosa, aliado à adição de CO 2 , otimiza a carotenogênese em H. pluvialis e aumenta a produção de astaxantina.Termos para indexação: astaxantina, carbono orgânico dissolvido, carotenoide, intensidade luminosa, microalga. Carotenogenesis in Haematococcus pluvialis cells induced by light and nutrient stressesAbstract -The objective of this work was to evaluate the responses of Haematococcus pluvialis cells to the carotenogenesis induction process, under light and nutrition stress. Cells were acclimated during 15 days in WC medium, with aeration with synthetic, filtered atmospheric air and flow rate of 100 mL min , photoperiod of 12 hours, and temperature of 23°C. The following two treatments were compared: cultivation under the described conditions, but with increase of light intensity up to 350 µmol photons m -2 s -1 ; and cultivation under the same conditions as the previous treatment, but with aeration containing 4% CO 2 . The treatments were done in triplicate, during ten days. With the addition of CO 2 and the increment in lighting, an increase was observed in the carotenoids/chlorophyll ratio and cell biomass. Cells stopped dividing on the second day of stress, when nitrate became limiting, and significantly increased their biovolume. The excretion of organic carbon and the concentration of astaxanthin increase in response to the addition of CO 2. Stress by light intensity combined with CO 2 addition optimizes carotenogenesis in H. pluvialis and increases astaxanthin production.

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“…Haematococcus synthesizes and accumulates high levels of astaxanthin under stress conditions such as high light irradiance and nitrogen starvation (Ceron et al, 2006;Gökpinar, 2005 and2010;Göksan, Ak, & Kılıç, 2011;Hagen, Grunevald, Xylander, & Rothe, 2001;Hata, Ogbonna, Hasegawa, Taroda, & Tana, 2001; Sim, 2005 and. An astaxanthin content of up to 5% in dry weight has been reported under commercial culture conditions (Aflola, Meshulam, Zarka, & Boussiba, 2007;Cifuentes, Gonzalez, Vargas, Hoeneisen, & Gonzalez, 2003;Orasa, Frangueira, Cid, & Abalde, 2000;Orasa, Valero, Herrero, & Abalde, 2001;Torzillo, Göksan, Faraloni, Kopecky, & Masojidek, 2003;Torzillo, Göksan, Işık, & Gökpınar, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…To date four Haematococcus species -H. pluvialis, H. lacustris, H. droebakensis and H. capensis with 11 strains have been cultivated in laboratory and commercial cultures. There have been several studies on H. pluvialis cultivation in Turkey Göksan et al, 2010;Göksan et al, 2011). In view of the economic importance of the genus, the present study describes the isolation and first examination in culture of a promising indigenous strain of Haematococcus for its astaxanthin content with a culture scale up in greenhouse raceway ponds .…”

Section: Introductionmentioning

confidence: 99%

Untitled

Turan¹

2019

Turk. J. Fish. Aquat. Sci.

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Samples of the green unicellular microalgae Haematococcus Flotow 1844 were collected from freshwater ponds in Homeros Valley, Bornova Creek, Izmir, Turkey. Specimens were isolated using the micropipetting method and were microscopically examined for morphological features. One isolate was identified as a local strain of Haematococcus sp. In addition, Scenedesmus acuminatus (Lagerheim) Chodat 1902, Scenedesmus dimorphus (Turpin) Kützing 1834 and Chlorella elipsodae Gernerck 1907 were found. DMSO (dimethyl sulfoxide) extracts of the local Haematococcus strain contained ~ 3.6 % total astaxanthin in dry weight. The isolate's culture was scaled up in greenhouse raceway ponds in batch mode and several parameters of biomass yield (cell density, optical density, dry weight content and chlorophyll a content) were measured during a two weeks growth trial. The Turkish isolate of Haematococcus sp. seems to be a promising candidate for the commercial production of astaxanthin for food and pharmaceuticals .

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An Alternative Approach to the Traditional Mixotrophic Cultures of Haematococcus pluvialis Flotow (Chlorophyceae)

Cited by 43 publications

References 0 publications

Influence of agriculture fertilizer for the enhanced growth and astaxanthin production from Haematococcus lacustris RRGK isolated from Himachal Pradesh, India

Influence of agriculture fertilizer for the enhanced growth and astaxanthin production from Haematococcus lacustris RRGK isolated from Himachal Pradesh, India

Carotenogênese em células de Haematococcus pluvialis induzidas pelos estresses luminoso e nutricional

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