An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Xu, Shunqing; Cao, Shasha; Zou, Bingjie; Yue, Yunyun; Gu, Chaohao; Chen, Xin; Wang, Pei; Dong, Xiaohua; Zheng, Xuelian; Li, Kai; Zhu, Min–Sheng; Zhao, Qingshun; Zhou, Guohua

doi:10.1186/s13059-016-1038-5

Cited by 26 publications

(27 citation statements)

References 34 publications

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“…The CRISPR/Cas9 system allows targeted genome editing, including frameshift knockout, gene insertion, and alteration, under the guidance of a specific guide RNA (gRNA) 1. Since the first system engineered from Streptococcus pyogenes Cas9 endonuclease,2 many Cas9 variants (e.g., Staphylococcus aureus Cas93), analogues (e.g., CRISPR/Cpf14 and FEN‐1/FokI fused endonuclease5) have emerged. To date, delivery of these gene editing systems relies mainly on viral vectors or electroporation 6.…”

Section: Introductionmentioning

confidence: 99%

HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute

Lao

Gao

et al. 2018

Advanced Science

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CRISPR/Cas9 technology enables targeted gene editing; yet, the efficiency and specificity remain unsatisfactory, particularly for the nonvirally delivered, plasmid‐based CRISPR/Cas9 system. To tackle this, a self‐assembled micelle is developed and evaluated for human papillomavirus (HPV) E7 oncogene disruption. The optimized micelle enables effective delivery of Cas9 plasmid with a transient transgene expression profile, benefiting the specificity of Cas9 recognition. Furthermore, the feasibility of using the micelle is explored for another nucleic acid‐guided nuclease system, Natronobacterium gregoryi Argonaute (NgAgo). Both systems are tested in vitro and in vivo to evaluate their therapeutic potential. Cas9‐mediated E7 knockout leads to significant inhibition of HPV‐induced cancerous activity both in vitro and in vivo, while NgAgo does not show significant E7 inhibition on the xenograft mouse model. Collectively, this micelle represents an efficient delivery system for nonviral gene editing, adding to the armamentarium of gene editing tools to advance safe and effective precision medicine‐based therapeutics.

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Section: Introductionmentioning

confidence: 99%

HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute

Lao

Gao

et al. 2018

Advanced Science

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“…() and Xu et al . () reported DNA‐guided and structure‐guided genome editing tools, respectively. We attempted both the methods; however, unfortunately, none of the methods showed detectable activity in B. mori (data not published).…”

Section: Current Prospective and Future Directionsmentioning

confidence: 99%

“…Although the two-component CRISPR/Cas9 system seems considerably simple for most trained molecular biologists, researchers continue to develop more genome editing tools. Gao et al (2016) and Xu et al (2016) reported DNAguided and structure-guided genome editing tools, respectively. We attempted both the methods; however, unfortunately, none of the methods showed detectable activity in B. mori (data not published).…”

Section: Simplicity and Efficiencymentioning

confidence: 99%

Genome editing in Bombyx mori: New opportunities for silkworm functional genomics and the sericulture industry

Smagghe

Xia

2018

Insect Science

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In recent years, research in life sciences has been remarkably revolutionized owing to the establishment, development and application of genome editing technologies. Genome editing has not only accelerated fundamental research but has also shown promising applications in agricultural breeding and therapy. In particular, the clustered, regularly interspaced, short palindromic repeat (CRISPR) technology has become an indispensable tool in molecular biology owing to its high efficacy and simplicity. Genome editing tools have also been established in silkworm (Bombyx mori), a model organism of Lepidoptera insects with high economic importance. This has remarkably improved the level and scope of silkworm research and could reveal new mechanisms or targets in basic entomology and pest management studies. In this review, we summarize the progress and potential of genome editing in silkworm and its applications in functional genomic studies for generating novel genetic materials.

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“…In addition to the CRISPR‐Cas system, Xu et al . engineered a novel structure‐guided endonuclease (SGN) to implement targeted DNA cleavage in vivo and in vitro .…”

Section: Perspectivesmentioning

confidence: 99%

Genome Engineering and Modification Toward Synthetic Biology for the Production of Antibiotics

Zou

Wang

et al. 2017

Medicinal Research Reviews

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Antibiotic production is often governed by large gene clusters composed of genes related to antibiotic scaffold synthesis, tailoring, regulation, and resistance. With the expansion of genome sequencing, a considerable number of antibiotic gene clusters has been isolated and characterized. The emerging genome engineering techniques make it possible towards more efficient engineering of antibiotics. In addition to genomic editing, multiple synthetic biology approaches have been developed for the exploration and improvement of antibiotic natural products. Here, we review the progress in the development of these genome editing techniques used to engineer new antibiotics, focusing on three aspects of genome engineering: direct cloning of large genomic fragments, genome engineering of gene clusters, and regulation of gene cluster expression. This review will not only summarize the current uses of genomic engineering techniques for cloning and assembly of antibiotic gene clusters or for altering antibiotic synthetic pathways but will also provide perspectives on the future directions of rebuilding biological systems for the design of novel antibiotics.

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An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease

Cited by 26 publications

References 34 publications

HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute

HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute

Genome editing in Bombyx mori: New opportunities for silkworm functional genomics and the sericulture industry

Genome Engineering and Modification Toward Synthetic Biology for the Production of Antibiotics

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