An alternative PCR-based method for monitoring thermal cycler performance

Uribe, Marcela R.; Adams, Sharon; Barracchini, Kathy; Hackett, Julia; Marincola, Francesco M.; Stroncek, David

doi:10.1016/j.humimm.2003.08.338

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Engineers can examine the effects of chip geometry, channel dimensions, and materials on temperature distribution during thermal cycling with simulations [46]. The software helps identify potential hotspots or temperature changes that could impact the PCR process by providing insights into variables, including convective heat transfer, conductive heat dissipation, and radiative heat exchange [47]. By offering a virtual environment to investigate and improve different thermal cycle factors, CFD considerably cuts down on the time and expense of experimental trial and error [48].…”

Section: Resultsmentioning

confidence: 99%

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Computation

View full text Add to dashboard Cite

Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for the detection of malaria. However, the collection and transportation of samples and the processing and dissemination of results via conventional PCR, especially when used for routine clinical practice, can hamper the technique’s sensitivity and specificity. The rampancy of such disease in the Philippines is aggravated by the limited supply of medical machinery and the poor economic state of the country; thus, the need to innovate a device for the early detection of malaria is necessary. With that, this study focuses on designing a microfluidic device that will mimic the function of a conventional genus-specific PCR based on the 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low-grade parasitemia. The design was intended to be portable, accessible, and economical, which none from past literature has dealt with specifically for malaria detection. This in silico design is a first in the country specially crafted for such reasons. The proposed device was developed and simulated using ANSYS software for Computational Fluid Dynamics (CFD) analyses. The simulation shows that adding loops to the design increases its relative deviation but minimally compared to having only a straight path design. This indicates that looping is acceptable in designing a microfluidic device to minimize chip length. It was also found that increasing the cross-sectional area of the fluid path decreases the efficiency of the design. Lastly, among the three materials utilized, the chip made of polypropylene is the most efficient, with a relative deviation of 0.94 compared to polycarbonate and polydimethylsiloxane, which have relative deviations of 2.78 and 1.92, respectively. Future researchers may mesh the 44-cycle microfluidic chip due to the limitations of the software used in this study, and other materials, such as biocomposites, may be assessed to broaden the application of the design.

show abstract

Section: Resultsmentioning

confidence: 99%

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Computation

View full text Add to dashboard Cite

show abstract

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Preprint

View full text Add to dashboard Cite

Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for the detection of malaria. However, the limitations of PCR especially when used for routine clinical practice can hamper its sensitivity and specificity. With that, this study focuses on designing a microfluidic device that will mimic the function of a conventional genus-specific PCR based on 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low grade parasitemia. The design was drawn and simulated using ANSYS 14.5 Computational Fluid Dynamics (CFD). The simulation shows that adding loops to the design increases its relative deviation but to a minimal extent as compared to having only a straight path design, which indicates that looping is acceptable in designing a microfluidic device to minimize chip length. Also, increasing the cross-sectional area of the fluid path decreases the efficiency of the design, thus, the design with a relatively smaller cross-sectional area is favored. And lastly, among the three materials utilized, the chip made of polypropylene is the most efficient with a relative deviation of 0.94 as compared to polycarbonate and polydimethylsiloxane which have relative deviations of 2.78 and 1.92, respectively.

show abstract

An alternative PCR-based method for monitoring thermal cycler performance

Cited by 2 publications

References 0 publications

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Contact Info

Product

Resources

About