An alternative RP‐HPLC method for the separation and determination of tocopherol and tocotrienol homologues as butter authenticity markers: A comparative study between two European countries

Abstract

Eight tocochromanols (a, b, g, and d homologues of tocopherol and tocotrienol) naturally occurring in foods were successfully separated within a 13-min run in the RP-HPLC mode. Analytes were separated on the Phenomenex Luna PFP column filled with the pentafluorophenyl stationary phase (3 mm, 150 mm  4.6 mm) using the mobile phase containing methanol:water (93:7 v/v) with an elution flow rate of 1 ml/min and column oven temperature of 40°C. The method was rapid, linear, accurate, and precise with detection limits in the range of 0.000184-0.000605 mg, preventing analyte losses due direct dissolution in 2-propanol. The developed RP-HPLC method in comparison with the NP-HPLC mode had a significantly higher sensitivity, speed, and repeatability, but primarily it protected against the loss of analytes and thus reduced the risk of possible error measurements. It was found that tocopherol contents in the tested butter samples amounted to 2.00-16.92 mg/100 g for samples coming from Poland and 2.61-2.98 mg/100 g for samples from Latvia, respectively. The method is characterized by simplicity of implementation and it was successfully applied in the determination of tocochromanols in butter to verify product authenticity.Practical applications: To ensure consumers' protection, food products should be subject to continuous quality and authenticity control. One way to determine butter authenticity is to analyze native tocochromanol contents. This paper describes a simple and fast method determine plant oils added to milk fat with the use of RP-HPLC techniques.à Details of separation conditions: column oven temperature 40°C and ambient temperature 21°C; flow rate 1 ml/min. Eur. J. Lipid Sci. Technol. 2014, 116, 895-903 Tocopherolsauthenticity markers of butter à Details of separation conditions: flow rate 1 ml/min; ambient temperature 21°C; mobile-phase MeOH/H 2 O, 93:7 v/v. Eur. J. Lipid Sci. Technol. 2014, 116, 895-903 Tocopherolsauthenticity markers of butter à Details of separation conditions: flow rate 1 ml/min; column over temperature 40°C and ambient temperature 21°C; mobile-phase MeOH/ H 2 O, 93:7 v/v. Eur. J. Lipid Sci. Technol. 2014, 116, 895-903 Tocopherolsauthenticity markers of butter

Phenolic compounds in different fruit parts of crab apple: Dihydrochalcones as promising quality markers of industrial apple pomace by-products

“…On this basis, a detailed analysis of phenolic compounds of powdered apple pomace, could help to ensure the high quality of the final product. Previously reported native bio-compounds, for instance, tocochromanols in margarin and butter, could be used as a potential quality markers for preliminary screening to exclude or confirm the possibility of adulteration (Górnaś and Siger, 2015;Górnaś et al, 2014c). In the present study, certain polyphenols, more specifically phloretin dihexoside and 3-hydroxyphloridzin, may be applied as a new tool (preliminary markers) to ensure the quality (apple pomace free of stems and seeds vs. apple pomace containing stems and seeds) and safety of the apple pomace (to exclude the possible presence of amygdalin, found in apple seeds).…”

Composition of tocochromanols in the kernels recovered from plum pits: the impact of the varieties and species on the potential utility value for industrial application

“…Tocopherol and tocotrienol homologues were determined using previously developed and validated method [25]. The chromatographic separation was carried out on the Shimadzu HPLC system (Shimadzu, Kyoto, Japan) consisting of a Luna PFP column (3 μm, 150 × 4.6 mm) with a guard column (4 × 3 mm) (Phenomenex, Torrance, CA, USA) and fluorescence detector (RF-10AXL) set at an excitation wavelength of 295 nm and emission wavelength of 330 nm.…”

New insights regarding tocopherols in Arabica and Robusta species coffee beans: RP-UPLC-ESI/MSn and NP-HPLC/FLD study

“…The NP-HPLC method provides a considerably better separation between b-T and g-T (Manzi et al, 1996). However, recent research papers (Gó rnaś et al, 2014b;Grebenstein and Frank, 2012;Inoue et al, 2012) describe the use of a column filled with pentafluorophenyl (PFP) groups bound to the silica surface stationary phase, which also ensures a good separation of b-T and g-T using the reversed phase method. Identification of tocopherols by the reversed phase in comparison to the normal phase provides a shorter time of analysis and better repeatability of retention times (Cert et al, 2000).…”