An amino acid substitution attributable to insecticide-insensitivity of acetylcholinesterase in a Japanese encephalitis vector mosquito, Culex tritaeniorhynchus

Nabeshima, Takeshi; Mori, Akio; Kozaki, Toshinori; Iwata, Yoichi; Hidoh, Osamu; Harada, Shizuko; Kasai, Shinji; Severson, David W.; Kono, Yoshiaki; Tomita, Takashi

doi:10.1016/j.bbrc.2003.11.141

Cited by 153 publications

(107 citation statements)

References 42 publications

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“…The G119S mutation that confers carbamate and organophosphate resistance in An. gambiae and Culex quinquefasciatus (19) was not detected in these samples, nor was the F455W mutation found in Cx tritaeniorhynchus (20). Several indels (from 2 to 45 nucleotides) were observed in the introns, but these were not correlated with resistance or geographical location.…”

Section: Resultsmentioning

confidence: 77%

Impact of pyrethroid resistance on operational malaria control in Malawi

Wondji

Coleman

Kleinschmidt

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

122

131

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The impact of insecticide resistance on insect-borne disease programs is difficult to quantify. The possibility of eliminating malaria in high-transmission settings is heavily dependent on effective vector control reducing disease transmission rates. Pyrethroids are the dominant insecticides used for malaria control, with few options for their replacement. Their failure will adversely affect our ability to control malaria. Pyrethroid resistance has been selected in Malawi over the last 3 y in the two major malaria vectors Anopheles gambiae and Anopheles funestus , with a higher frequency of resistance in the latter. The resistance in An. funestus is metabolically based and involves the up-regulation of two duplicated P450s. The same genes confer resistance in Mozambican An. funestus , although the levels of up-regulation differ. The selection of resistance over 3 y has not increased malaria transmission, as judged by annual point prevalence surveys in 1- to 4-y-old children. This is true in areas with long-lasting insecticide-treated nets (LLINs) alone or LLINs plus pyrethroid-based insecticide residual spraying (IRS). However, in districts where IRS was scaled up, it did not produce the expected decrease in malaria prevalence. As resistance increases in frequency from this low initial level, there is the potential for vector population numbers to increase with a concomitant negative impact on control efficacy. This should be monitored carefully as part of the operational activities in country.

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Section: Resultsmentioning

confidence: 77%

Impact of pyrethroid resistance on operational malaria control in Malawi

Wondji

Coleman

Kleinschmidt

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

122

131

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“…The second amino acid substitution associated with insensitive AChE-mediated insecticide resistance in mosquitoes, an F455W substitution (T. californica nomenclature), was reported for Cx. tritaeniorhynchus (Nabeshima et al, 2004). A third amino acid substitution, F290V, associated with insensitive AChE-mediated insecticide resistance, was recently reported for Cx.…”

Section: Ace1 Cdnamentioning

confidence: 92%

“…However, the F455W substitution in Cx. tritaeniorhynchus (Nabeshima et al, 2004) does reflect two independent mutation events (TTT to TGG). No broad investigation of Ace1 polymorphism among Cx.…”

Section: Ace1 Cdnamentioning

confidence: 98%

“…The Ace1 homolog in Cx. tritaeniorhynchus (listed as Ace2, Nabeshima et al, 2004) and likely also in Cx. pipiens (Malcolm et al, 1998) is located in the orthologous genome region, although the two Culex spp.…”

Section: Ace1 Genome Locationmentioning

confidence: 99%

“…pipiens (Alout et al, 2007;Weill et al, 2002Weill et al, , 2003, Anopheles albimanus (Weill et al, 2004a), and Cx. tritaeniorhynchus (Nabeshima et al, 2004). The amino acid coding sequence was reported for the mosquito, Aedes aegypti, Ace1 gene, although insensitive AChE-mediated insecticide resistance is rare in natural Ae.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis

Mori

Lobo

deBruyn

et al. 2007

Insect Biochemistry and Molecular Biology

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Insensitive acetylcholinesterase (AChE) has been shown to be responsible for resistance to organophosphates and carbamates in a number of arthropod species. Some arthropod genomes contain a single Ace gene, while others including mosquitoes contain two genes, but only one confers insecticide resistance. Here we report the isolation of the full-length cDNA and characterization of the complete genomic DNA sequence for the Ace1 gene in the yellow fever mosquito, Aedes aegypti. The Ace1 homolog in other mosquito species has been associated with insecticide resistance. The full-length cDNA consists of 2,721 bp and contains a 2,109 bp open reading frame that encodes a 702 amino acid protein. The amino acid sequence is highly conserved with that of other mosquitoes, including greater than 90% identity with Culex spp. and about 80% identity with Anopheles gambiae. The genomic DNA sequence includes 138,970 bp and consists of eight exons with seven introns ranging from 59 to 114,350 bp. Exons 2 and 8 show reduced amino acid conservation across mosquito species, while exons 3-7 are highly conserved. The Ace1 introns in Ae. aegypti reflect a high frequency of repetitive sequences that comprise about 45% of the total intron sequence. The Ace1 locus maps to the p-arm of chromosome 3, which corresponds to the orthologous genome regions in Culex spp. and An. gambiae.

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RNA INTERFERENCE OF TWO ACETYLCHOLINESTERASE GENES IN Plutella xylostella REVEALS THEIR DIFFERENT FUNCTIONS

Sun

2012

Arch Insect Biochem Physiol

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Acetylcholinesterase (AChE, EC 3.1.1.7) is an important enzyme with a typical function of degrading the neurotransmitter acetylcholine. Although two ace genes were reported in Plutella xylostella, their function differences remain largely unknown. The chemically synthesized siRNAs (si-Pxace1 and si-Pxace2) were injected into the second instar larvae to knock down Pxace1 and Pxace2, either respectively or simultaneously. The mRNA abundance of Pxace1 and Pxace2 was significantly reduced to 7-33.5% of the control levels at 72 h after siRNA injection. The AChE activities were significantly decreased at 96 h after treatment. Silencing of Pxace1 or Pxace2 resulted in mortality of 33.9 and 22.9%, respectively. The survivors in siRNA-treated groups had apparent growth inhibition such as reduction in larvae weights and lengths, malformation and motor retardation. Knockdown of Pxace1 apparently affected more on larvae growth than that of Pxace2, suggesting that Pxace1 had more important roles than Pxace2. Both Pxace1 and Pxace2 genes might have atypical functions in regulating larvae growth and motor ability.

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An amino acid substitution attributable to insecticide-insensitivity of acetylcholinesterase in a Japanese encephalitis vector mosquito, Culex tritaeniorhynchus

Cited by 153 publications

References 42 publications

Impact of pyrethroid resistance on operational malaria control in Malawi

Impact of pyrethroid resistance on operational malaria control in Malawi

Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis

RNA INTERFERENCE OF TWO ACETYLCHOLINESTERASE GENES IN Plutella xylostella REVEALS THEIR DIFFERENT FUNCTIONS

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