An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper

Abstract

Characterization and genetic engineering of plant transporters involved in the pesticide uptake and translocation facilitate pesticide relocation to the tissue where the pests feed, thus improving the bioavailability of the agrichemicals. We aimed to identify thiamethoxam (THX) transporters in rice and modify their expression for better brown planthopper (BPH) control with less pesticide application. A yeast library expressing 1385 rice transporters was screened, leading to the identification of an amino acid transporter-like (ATL) gene, namely OsATL15, which facilitates THX uptake in both yeast cells and rice seedlings. In contrast to a decrease in THX content in osatl15 knockout mutants, ectopic expression of OsATL15 under the control of the CaMV 35S promoter or a vascular-bundle-specific promoter gdcsPpro significantly increased THX accumulation in rice plants, thus further enhancing the THX efficacy against BPH. OsATL15 was localized in rice cell membrane and abundant in the root transverse sections, vascular bundles of leaf blade, and stem longitudinal sections, but not in hull and brown rice at filling stages. Our study shows that OsATL15 plays an essential role in THX uptake and its systemic distribution in rice. OsATL15 could be valuable in achieving precise pest control by biotechnology approaches.

Disruption ofOsLAT5is sufficient to endow rice tolerance to dihydropyridine herbicides at commercial application concentrations

“…Uptake refers to the process of pesticides entering the plant, which can be achieved through the roots or leaves; whereas, pesticide transport in plants is generally divided into intracellular and inter-organ transport [45][46][47][48] . Recently, OsATL15 was first identified to facilitate thiamethoxam uptake and systemic distribution in rice, which further confirmed that plant transporters may participate in pesticide entry into plants 49 . To take effect, herbicides must enter the plant, be translocated, and reach their target site 3 , and this process involves transporters.…”

A nano‐delivery system expands the insecticidal target of thiamethoxam to include a devastating pest, the fall armyworm

“…, 2015; Xiao et al. , 2022). Our previous study demonstrated that the dendrimer‐loaded TMX showed enhanced toxicity against the non‐target pest Helicoverpa armigera (cotton bollworm) (Liu et al.…”

Comparison of Glyphosate-Degradation Ability of Aldo-Keto Reductase (AKR4) Proteins in Maize, Soybean and Rice

“…To detect the localization of OsAKR4-1, the OsAKR4-1-GFP plasmid was transfected into the rice protoplast for screening the fluorescence signal, using a Leica SP8 confocal laser scanning microscope as the parameter mentioned previously [ 47 ].…”

“…In brief, all tissues were incubated overnight at 37 °C and then cleared in the 70% ethanol. The GUS-staining results were observed using a stereoscope, as described previously [ 47 ].…”