An amperometric cholesterol biosensor based on multiwalled carbon nanotubes and organically modified sol-gel/chitosan hybrid composite film

Tan, Xuecai; Li, Minjian; Cai, Peng; Luo, Lijun; Zou, Xiaoyong

doi:10.1016/j.ab.2004.10.040

Cited by 264 publications

(99 citation statements)

References 55 publications

(87 reference statements)

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“…-6 до 4,5×10 -4 моль/л) и предел его обнаружения (4×10 -6 моль/л) при использовании стеклоуглеродного электрода, не содержавшего углеродные нанотрубки [27].…”

Section: сенсоры с нанесением углеродных нанотрубокunclassified

“…Технологии изготовления электрохимических сенсоров путём нанесения углеродных нанотрубок на рабочую поверхность электрода с помощью полиэтиленимина [2][3][4][5][6][7], органических красителей [9,[11][12][13] и циклодекстринов [14][15][16][17] схожи между собой: нанотрубки распределяются в растворах перечисленных химических соединений, образуя гомогенную суспензию, которая далее наносится на поверхность электрода с последующим высыханием. Хитозан [18][19][20][21][22], белковые соединения [23,24], ионные жидкости [25,26], гели [27,28] и тиолы [29][30][31] также могут использоваться для нанесения углеродных нанотрубок на рабочую поверхность стеклоуглеродного, золотого или др. электрода.…”

Section: сенсоры с нанесением углеродных нанотрубокunclassified

“…Получаемые сенсоры позволяют определять большое число химических соединений, являющихся характерными для биомедицинских исследований, в частности дофамин [2-4, 11, 15, 19, 26, 43], допак [2,4], адреналин [4,15,35], норадреналин [4,17,20], аскорбиновая [2,7,19] и мочевая [16,20] кислоты, пероксид водорода [2,4,5,22,25,[27][28][29][36][37][38], NADH [21,44], глюкоза [2,21,22,28,29,[36][37][38][39]44], фруктоза [44], холестерин [27], цитохром с [30], амитрол [4,5], диурон [5], монурон [5], хлортолурон [5], офлоксацин [12], эстрадиол [13], тимин …”

Section: сенсоры с нанесением углеродных нанотрубокunclassified

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Electrochemical sensors based on carbon nanotubes and their use in biomedical research.Part 2: sensors manufactured by dispersion of carbon nanotubes by means of polyethyleneimine, organic dyes, cyclodextrins, chitosan, proteins, room-temperature ionic liquids, gels, and thiols. Sensors manufactured by dispersion of carbon nanotubes by electropolymerization process. sensors manufactured by dispersion of carbon nanotubes by layer-by-layer deposition

Va¹

2012

BIOMED KHIM

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Electrochemical sensors based on carbon nanotubes are widely distributed in biomedical researches. One group of these sensors contains the sensors manufactured by dispersion of carbon nanotubes on an electrode surface by means of polyethyleneimine, organic dyes, cyclodextrins, chitosan, proteins, room-temperature ionic liquids, gels, thiols, by electropolymerization process, and by layer-by-layer deposition. The development directions of such sensors are analyzed. The general information on manufacturing techniques of these sensors is submitted. The opportunities of these sensors for carrying out biomedical researches are demonstrated.

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Section: сенсоры с нанесением углеродных нанотрубокunclassified

See 1 more Smart Citation

Electrochemical sensors based on carbon nanotubes and their use in biomedical research.Part 2: sensors manufactured by dispersion of carbon nanotubes by means of polyethyleneimine, organic dyes, cyclodextrins, chitosan, proteins, room-temperature ionic liquids, gels, and thiols. Sensors manufactured by dispersion of carbon nanotubes by electropolymerization process. sensors manufactured by dispersion of carbon nanotubes by layer-by-layer deposition

Va¹

2012

BIOMED KHIM

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“…Some reports have described the use of chitosan for the immobilization of glutamate dehydrogenase on glassy carbon [15][16][17][18], gold [19] and platinum [20] electrodes. Both reagentless and non-reagentless conventional sized glutamate biosensors have previously been developed by drop-coating the required biomolecules onto the working electrode of a screen-printed carbon electrode [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring

Hughes

Pemberton

Fielden

et al. 2016

Analytica Chimica Acta

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Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights. UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. A mixture of the enzyme glutamate dehydrogenase (GLDH), cofactor nicotinamide adenine dinucleotide (NAD + ) and the biopolymer chitosan (CHIT) were drop-coated onto the surface of the transducer (MB-SPCE) in a simple one step fabrication process.The reagentless biosensor was used with amperometry in stirred solution at an applied potential of +0.1 V (vs. Ag/AgCl). All experiments were carried out at the following conditions: pH 7, temperature 37ºC, atmosphere 5% CO 2 .The linear range of the device was found to be 25 -125 μM in phosphate buffer (75 mM, containing 0.05 M NaCl) and 25 -150 μM in cell culture medium. The limits of detection (LOD) were found to be 1.2 µM and 4.2 µM based on three times signal to noise, using PBS and culture medium respectively. The sensitivity was calculated to be 106 nA µM -1 cm -2 and 210 nA µM -1 cm -2 in PBS and cell medium respectively. The response time was ~60s in an agitated solution.HepG2 cells were exposed to various concentrations of paracetamol (1 mM, 5 mM and 10 mM) in order to investigate the drug-induced release of glutamate into the culture medium in real time. Two toxicity studies were investigated using different methods of exposure and analysis.The first method consisted of a single measurement of the glutamate concentration, using the method of standard addition, after 24 hours incubation. The concentrations of glutamate were 3 found to be 52µM, 93µM and 177µM, released on exposure to 1mM, 5mM and 10mM paracetamol respectively.The second method involved the continuous monitoring of glutamate released from HepG2 cells upon exposure to paracetamol over 8 hours. The concentrations of glutamate released in the presence of 1mM, 5mM and 10mM paracetamol, increased in proportion to the drug concentration, ie: 16µM, 28µM and 62µM respectively. This result demonstrates the feasibility of using this approach to monitor early metabolic changes after exposure to a model toxic compound.

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“…[15][16][17] Cholesterol is essential for many metabolic processes, but high levels are often associated with heart disease. To know of its level in food as well as in our body, a number of papers related to electrochemical cholesterol sensors have been published based on enzymes, [18][19][20][21][22] molecularly imprinted polymers, 16,17,23,24 and other non-enzymatic approaches. 25,26 This article presents a SAM-based sensor optimized for food cholesterol.…”

Section: Introductionmentioning

confidence: 99%