An amperometric glucose biosensor based on layer-by-layer GOx-SWCNT conjugate/redox polymer multilayer on a screen-printed carbon electrode

Gao, Qiang; Guo, Yanyan; Zhang, Wenyan; Qi, Honglan; Zhang, Chengxiao

doi:10.1016/j.snb.2010.10.034

Cited by 83 publications

(35 citation statements)

References 61 publications

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“…4A, Inset, 95% of steadystate current) with an average sensing time of ∼1.1 s, which rivals the performance of the fastest reported response time (∼0.2 s) of glucose sensor made with a single PAni nanojunction (37), and superior to that based on PAni nanotube array (∼3 s) (38). The sensitivity of our PAni hydrogel glucose sensor is 16.7 μA· mM −1 , and the sensitivity per unit area is 85.4 μA·mM −1 · cm −2 , which are higher than the reported glucose sensors based on other PAni nanostructures (37), polypyrrole (39), carbon nanotubes (40), and single walled carbon nanotubes (41). The exceptionally fast response and high sensitivity are again attributed to the relatively short diffusion path (thus favoring molecular and electronic transport) that is due to the open channels of hierarchical nanostructure and the continuously conductive framework of the PAni hydrogel.…”

Section: Resultsmentioning

confidence: 62%

Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity

Pan

Yu²,

Zhai

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

1,091

895

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Conducting polymer hydrogels represent a unique class of materials that synergizes the advantageous features of hydrogels and organic conductors and have been used in many applications such as bioelectronics and energy storage devices. They are often synthesized by polymerizing conductive polymer monomer within a nonconducting hydrogel matrix, resulting in deterioration of their electrical properties. Here, we report a scalable and versatile synthesis of multifunctional polyaniline (PAni) hydrogel with excellent electronic conductivity and electrochemical properties. With high surface area and three-dimensional porous nanostructures, the PAni hydrogels demonstrated potential as high-performance supercapacitor electrodes with high specific capacitance (∼480 F·g −1 ), unprecedented rate capability, and cycling stability (∼83% capacitance retention after 10,000 cycles). The PAni hydrogels can also function as the active component of glucose oxidase sensors with fast response time (∼0.3 s) and superior sensitivity (∼16.7 μA·mM −1 ). The scalable synthesis and excellent electrode performance of the PAni hydrogel make it an attractive candidate for bioelectronics and future-generation energy storage electrodes.conductive polymer hydrogel | supercapacitors | biosensors H ydrogels are polymeric networks that have a high level of hydration and three-dimensional (3D) microstructures bearing similarities to natural tissues (1, 2). Hydrogels based on conducting polymers [e.g., polythiophene, polyaniline (PAni), and polypyrrole] combine the unique properties of hydrogels with the electrical and optical properties of metals or semiconductors (3-6) thus offering an array of features such as intrinsic 3D microstructured conducting frameworks that promote the transport of charges, ions, and molecules (7). Conducting polymer hydrogels provide an excellent interface between the electronictransporting phase (electrode) and the ionic-transporting phase (electrolyte), between biological and synthetic systems, as well as between soft and hard materials (8). As a result, conducting polymer hydrogels have demonstrated great potential for a broad range of applications from energy storage devices such as biofuel cells and supercapacitors, to molecular and bioelectronics (9) and medical electrodes (8).To date, the synthetic routes toward conducting polymer hydrogels include synthesizing a conducting polymer monomer within a nonconducting hydrogel matrix (8, 9) using multivalent metal ions (Fe 3þ or Mg 2þ ) to crosslink poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) (10, 11) and using nonconducting poly(ethylene glycol) diglycidyl ether, or poly(styrenesulfonate) to crosslink PAni (12, 13); however, nonconducting hydrogel matrix and polymers result in the deterioration of the electrical properties, whereas excessive metal ions may reduce the biocompatibility of hydrogels. Moreover, there have yet been any reports in regard to conductive hydrogels that can be facilely micropatterned, which is important for fabricating hy...

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Section: Resultsmentioning

confidence: 62%

Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity

Pan

Yu²,

Zhai

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

1,091

895

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“…An attractive approach to tackle this challenge is to use a polymeric mediator which has mediator moieties chemically attached to polymer chains. Because of its large molecular size, the polymeric mediator can be co-immobilized with enzyme at electrode by various means, including surface grafting, [31][32][33][34] layer-by-layer surface adsorption, [35][36][37] retention behind semi-permeable dialysis membranes, [38][39][40] physical entrapment [41][42][43] or cross-link in hydrogels, [44][45][46][47] entrapment in electropolymerized [48,49] or chemically formed layers [50] or in inorganic layers, [51,52] and blend in carbon pastes. [53] At LifeScan Scotland Limited, we have synthesized a ferrocene polymeric mediator which is a copolymer of vinylferrocene, acrylamide and 2-(diethylamino)ethyl methacrylate.…”

Section: Fad-gdh Biosensor For Continuous Glucose Monitoringmentioning

confidence: 99%

Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

Cardosi¹,

Liu²

2012

Dehydrogenases

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“…Therefore increased effective reacting area is one way to obtain a higher sensitivity. Different research groups apply many different techniques to increase the effective surface area including the use of materials like carbon nanotubes and processes like nano-templating [10,[13][14][15][16][17]. In this research, we have investigated developing a nano-scale corrugated conductive polymer surface suitable for immobilizing glucose oxidase (GO x ) for the development of a low-cost nano-biosensor.…”

Section: Introductionmentioning

confidence: 99%

Functional design of electrolytic biosensor

Preethichandra

Ekanayake

Onoda

2017

J. Phys.: Conf. Ser.

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Abstract.A novel amperometric biosensbased on conjugated polypyrrole (PPy) deposited on a Pt modified ITO (indium tin oxide) conductive glass substrate and their performances are described. We have presented a method of developing a highly sensitive and low-cost nanobiosensor for blood glucose measurements. The fabrication method proposed decreases the cost of production significantly as the amount of noble metals used is minimized. A nanocorrugated PPy substrate was developed through pulsed electrochemical deposition. The sensitivity achieved was 325 mA/(Mcm 2 ) and the linear range of the developed sensor was 50-60 mmol/l. Then the application of the electrophoresis helps the glucose oxidase (GO x ) on the PPy substrate. The main reason behind this high enzyme loading is the high electric field applied across the sensor surface (working electrode) and the counter electrode where that pushes the nano-scale enzyme particles floating in the phosphate buffer solution towards the substrate. The novel technique used has provided an extremely high sensitivities and very high linear ranges for enzyme (GO x ) and therefore can be concluded that this is a very good technique to load enzyme onto the conducting polymer substrates.

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An amperometric glucose biosensor based on layer-by-layer GOx-SWCNT conjugate/redox polymer multilayer on a screen-printed carbon electrode

Cited by 83 publications

References 61 publications

Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity

Hierarchical nanostructured conducting polymer hydrogel with high electrochemical activity

Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

Functional design of electrolytic biosensor

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