An amperometric oxalate biosensor based on sorghum oxalate oxidase bound carboxylated multiwalled carbon nanotubes–polyaniline composite film

Yadav, Sandeep; Devi, Rekha; Kumari, Santosh; Yadav, Sarita; Pundir, C.S.

doi:10.1016/j.jbiotec.2010.12.008

Cited by 56 publications

(26 citation statements)

References 37 publications

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“…[27][28][29][30] It is important to note that there are no known redox mediators for oxalate oxidase, so all oxalate oxidase biosensors have had to operate via the detection of the hydrogen peroxide enzymatic product. Designs include electrochemical detection of oxalate using edge plane and basal plane pyrolytic graphite electrodes 28 where there was a high overpotential and electrochemical detection of H 2 O 2 using multiwalled carbon nanotube-gold nanoparticle composites, 28 carboxylated multi-walled carbon nanotubes in a polyaniline composite film, 30 and chromium hexacyanoferrate 27 as catalysts. Here, cyclic voltammetry was used to examine the use of TEMPO with oxalate and OxOx in phosphate buffer at pH 5.2 ( Figure 5).…”

Section: Resultsmentioning

confidence: 99%

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TEMPO as a Promising Electrocatalyst for the Electrochemical Oxidation of Hydrogen Peroxide in Bioelectronic Applications

Abdellaoui

Knoche

Lim

et al. 2015

J. Electrochem. Soc.

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A number of bioelectronic applications work with oxidase enzymes and many of them can operate with small molecule or polymer redox mediators. However, for some oxidases, there are no known redox mediators able to mediate electron transfer. Therefore, electron transfer must occur through peroxide production and oxidation at the electrode surface. Organic redox catalysts such as oxoammonium cations, are able to oxidize H 2 O 2 to form nitroxyl radicals, which can be electro-oxidized and regenerate the oxoammonium cation form. In this study, we investigate the ability to use TEMPO as a platform for the electrocatalytic oxidation of H 2 O 2 at different pHs. The results have shown that TEMPO can be used to monitor H 2 O 2 in broad pH range (≥4) at 530 mV (vs SCE). Combining TEMPO with cholesterol oxidase, we have shown the possibility to monitor the cholesterol oxidation with a linear range between 20 μM and 2.5 mM with a sensitivity of 54.86 mA cm −2 M −1 . Furthermore, we have studied the electrocatalytic oxidation of oxalate by oxalate oxidase for biofuel cell applications. These combined results demonstrate TEMPO as a promising electrocatalyst applied for the development of electrochemical biosensors or enzymatic biofuel cells. Hydrogen peroxide (H 2 O 2 ) is an enzymatic product of oxygen (O 2 ) reduction, which can be catalyzed by an exhaustive list of oxidase enzymes including glucose oxidase, alcohol oxidase, lactate oxidase, urate oxidase, cholesterol oxidase, D-amino acid oxidase, glutamate oxidase, lysine oxidase, and oxalate oxidase. Hydrogen peroxide is the smallest and simplest peroxide and is of great interest in multiple fields as a disinfectant, as a propellant in the aerospace industry, and as a biomarker for biological decomposition in the food industry.1 It also applied in the defense system of some insects, 2 the immune system, 3 and regulation of cellular processes. 4 Development of enzymatic biosensors presents a significant utility for the detection and quantification of the large number of oxidase substrates for fundamental studies as well as diagnostic and industry applications. Different techniques for the detection of oxidase substrates that have been described in the literature include spectrophotometry, 5 fluorimetry, 6 chemiluminescence, 7 and fluorescence, 8 but most of them are costly and time consuming. Hydrogen peroxide can be oxidized electrochemically, and thus electrochemical techniques have also been used as detection methods. Electrochemistry is commonly described as a simpler, cheaper, faster, and more sensitive detection technique for the development of oxidase substrate biosensors and enzymatic biofuel cells. 9,10The direct electrochemical oxidation and reduction of H 2 O 2 require high overpotentials (>+0.65 V for oxidation and >−1.7 V for reduction versus NHE). These high potentials limit analytical applications involving the oxidation or reduction of H 2 O 2 in complex media, because media electrolysis causes interference and can foul the electrode surface. In order to d...

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Section: Resultsmentioning

confidence: 99%

“…Other oxalate biosensors only measure in the μM range. 27 TEMPO, therefore, has possible applications in an oxalate biosensor where it enhances the H 2 O 2 signal. Fitting the curve to traditional Michaelis-Menten kinetic parameters gives a calculated K M of 3.96 ± 0.56 mM.…”

Section: Resultsmentioning

confidence: 99%

TEMPO as a Promising Electrocatalyst for the Electrochemical Oxidation of Hydrogen Peroxide in Bioelectronic Applications

Abdellaoui

Knoche

Lim

et al. 2015

J. Electrochem. Soc.

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“…In another interesting report, the synergistic electrochemical effect of Au-Pt NPs/nanoPANI/CS has been investigated to develop an improved amperometric H 2 O 2 biosensor. 18 21 revealed that the synergistic effect of GR-CNT hybrid materials, Au-Pt NPs, and the enzymatically induced deposition of PANI can provide an efficient platform for the design of novel electrochemical biosensors. Peroxide biosensors with enhanced stability and eight times more sensitivity were reported with HRP modified CNT doped PANI films.…”

Section: Polyaniline-based Biosensors Polyaniline-based Peroxide Biosmentioning

confidence: 99%

Polyaniline-based biosensors

Malhotra¹,

Dhand²,

Lakshminarayanan³

et al. 2015

NDD

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Biosensors are the subject of an immensely growing field of research owing to their broad range of applications in medicines, pharmacy, environmental monitoring, food and process control, defense and security, and principally in diagnostics. Diverse materials have been investigated for the advancement of biosensors in terms of their miniaturization, sophistication, cost, biosensing features, ie, detection limit, sensitivity, stability, selectivity, etc. Polyaniline (PANI) is one of the most interesting conductive polymers for biosensor design in view of its excellent electrochemical properties (polyelectrocatalytic characteristics, reversible redox behavior, and electrochemical tunability), straightforward processability, long-term environmental stability, and functionality-rich chemical structure. In this review, an attempt is made to compile almost all the existent literature on PANI-based biosensors in terms of enzymatic biosensors (for H 2 O 2 , glucose, cholesterol, phenol/polyphenol/catecholamine detection), genosensors (DNA sensing), and immunosensors from 2006 to 2015. Furthermore, reports available on the biosensing of urea, uric acid, creatinine, pesticides, amino acids, and other clinically significant analytes are also assembled to provide a comprehensive overview on PANI-based biosensors.

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“…Commercial applications have been rather slow to develop, however, primarily because of the high production costs of the best quality nanotubes. For these reasons they have attracted huge academic and industrial interest, with several articles on nanotubes being published the lasts years (Ruoff & Lorents, 1995;Gouveia-Caridade et al, 2008;Yadav et al, 2011).…”

Section: Carbon Nanotubesmentioning

confidence: 99%