An amphiphilic aggregate-induced emission polyurethane probe for in situ actin observation in living cells

Niu, Yuqing; Zhang, Bokai

doi:10.1016/j.jcis.2020.08.113

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…At 12 h seeding, the sterilized nanofiber film samples (15 mm in diameter) were added in to each pore of the TCP and cultured for 24 and 48 h followed by staining using a live/dead assay kit (L3224, Invitrogen) using previously described methods [ 32 , 33 ]. In short, after sucking and discarding the culture medium, we washed the cells and nanofiber film samples in each well plate well with 1× PBS, then added 1 mL staining solution containing 0.4 μL Calcein AM and 2 μL ether-homodimer-1 to each well, and incubated in the dark for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Niu

2021

Nanomaterials

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In this study, we designed and synthetized artificial vascular scaffolds based on nanofibers of collagen functionalized with hyaluronic acid (HA) in order to direct the phenotypic shape, proliferation, and complete endothelization of mouse primary aortic endothelial cells (PAECs). Layered tubular HA/collagen nanofibers were prepared using electrospinning and crosslinking process. The obtained scaffold is composed of a thin inner layer and a thick outer layer that structurally mimic the layer the intima and media layers of the native blood vessels, respectively. Compared with the pure tubular collagen nanofibers, the surface of HA functionalized collagen nanofibers has higher anisotropic wettability and mechanical flexibility. HA/collagen nanofibers can significantly promote the elongation, proliferation and phenotypic shape expression of PAECs. In vitro co-culture of mouse PAECs and their corresponding smooth muscle cells (SMCs) showed that the luminal endothelialization governs the biophysical integrity of the newly formed extracellular matrix (e.g., collagen and elastin fibers) and structural remodeling of SMCs. Furthermore, in vitro hemocompatibility assays indicated that HA/collagen nanofibers have no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular implants.

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Section: Methodsmentioning

confidence: 99%

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Niu

2021

Nanomaterials

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“…To date, there has been relatively few investigations on the mechanism of PU particles. Y. Niu et al [19] have described that PU nanoparticles flanked by polyethylene glycol and polycaprolactone segments have the ability to undergo endocytic uptake, followed by endosomal escape.…”

Section: Introductionmentioning

confidence: 99%

The Influence of an Isocyanate Structure on a Polyurethane Delivery System for 2′-Deoxycytidine-5′-monophosphate

Borcan,

Vlase,

Vlase

et al. 2023

JFB

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The delivery of nucleosides represents an interesting research trend in recent years due to their application in various viral infections. The main aims of this study were to develop and to characterize polyurethane particles that are intended to be used for the transport of nucleosides. Three samples have been prepared using aliphatic diisocyanates, a mixture of polyethylene glycol, polycaprolactone, and diols, respectively. The samples were characterized through refractivity measurements, drug loading efficacy, release and penetration rate investigations, FTIR and Raman spectroscopy, thermal analyses, Zetasizer, SEM, HDFa cells viability, and irritation tests on mice skin. The results indicate the obtaining of particles with sizes between 132 and 190 nm, positive Zeta potential values (28.3–31.5 mV), and a refractivity index around 1.60. A good thermal stability was found, and SEM images show a medium tendency to agglomerate. The samples’ color, pH, and electrical conductivity have changed only to a small extent over time, and the evaluations indicate an almost 70% encapsulation efficacy, a prolonged release, and that around 70% of particles have penetrated an artificial membrane in the first 24 h. The synthesized products should be tested in further clinical trials, and the current tests on cell cultures and mice skin revealed no side effects.

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“…Building on the previous knowledge on formation of AIE nanostructures [1,5,9,22], this study provides a molecularly detailed description on the assembly and arrangement of the AIE polymers at air/liquid interfaces. 2D nanostructures from a series of amphiphilic block copolymers PEGmb-P(TPE-TMC)n were achieved by using the Langmuir technique.…”

Section: Discussionmentioning

confidence: 99%

“…[21] Different blocks have been tested in AIE-copolymers, e.g., polyurethane for selective imaging of actin in living cells. [22] Block copolymers (PEG)m-b-P(TPE-TMC)n combining polyethylene glycol (PEG)m and polytrimethylene carbonate P(TPE-TMC)n have been proven to efficiently self-assemble in bulk solution to polymersomes displaying AIE in our previous work. [23] The PEG block provides adequate hydrophilicity to achieve amphiphilic block copolymer, while simultaneously being biocompatible and repellent for adsorption of proteins.…”

Section: Introductionmentioning

confidence: 99%

Amphiphilic polymers for aggregation-induced emission at air/liquid interfaces

Argudo

Zhang

Chen

et al. 2021

Journal of Colloid and Interface Science

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An amphiphilic aggregate-induced emission polyurethane probe for in situ actin observation in living cells

Cited by 7 publications

References 38 publications

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

The Influence of an Isocyanate Structure on a Polyurethane Delivery System for 2′-Deoxycytidine-5′-monophosphate

Amphiphilic polymers for aggregation-induced emission at air/liquid interfaces

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