An analysis of esterase activities from surgeonfish tissues yields evidence of an atypical pseudocholinesterase

Leibel, Wayne S.

doi:10.1016/0305-0491(88)90003-x

Cited by 15 publications

(12 citation statements)

References 36 publications

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“…It was found in earlier studies that certain fish ChEs combine properties of (mammalian) AChE and BChE [14–17]. There has been some controversy concerning how to classify such atypical fish ChEs [14,15,17]. Recent progress in the genetics of ChEs revealed that two genes encoding ChEs exist in higher vertebrates, AChE and BChE [2].…”

Section: Discussionmentioning

confidence: 99%

Different sensitivity to organophosphates of acetylcholinesterase and butyrylcholinesterase from three‐spined stickleback (Gasterosteus aculeatus): Application in biomonitoring

Sturm

Wogram

Segner

et al. 2000

Enviro Toxic and Chemistry

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Different cholinesterases from brain and body muscle of the fish three‐spined stickleback (Gasterosteus aculeatus) were investigated concerning their potential use in the biomonitoring of organophosphate insecticides (OPs). Stickleback brain contained acetylcholinesterase (AChE) but no butyrylcholinesterase (BChE). Body muscle contained both AChE and BChE. In vitro, body muscle BChE was three orders of magnitude more sensitive than brain or body muscle AChE to the OPs paraoxon and dichlorvos. In 1998, cholinesterase activities were recorded in stickleback from eight streams differing in pesticide contamination. Automated on‐site samplers that selectively collect water during runoff events were used to obtain water samples for pesticide analysis. The OP parathion was found in stream water sampled during runoff events in five cases. The variation of brain and body muscle AChE activity between streams was not related to water contamination by pesticides. Body muscle BChE was strongly decreased (>85%) in stickleback collected at all streams with previous parathion contamination events. A significant decrease in BChE (78%) occurred also, however, at one stream where other pesticides but no OPs were found. Because stickleback AChE and BChE differed strongly in their in vitro sensitivity and their response in the field, these enzymes should be considered separately in studies with fish.

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Section: Discussionmentioning

confidence: 99%

Different sensitivity to organophosphates of acetylcholinesterase and butyrylcholinesterase from three‐spined stickleback (Gasterosteus aculeatus): Application in biomonitoring

Sturm

Wogram

Segner

et al. 2000

Enviro Toxic and Chemistry

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show abstract

“…The differences in tissue enzyme activities and pH profiles for both AChE and BChE show the existence of at least 2 different enzymes acting on synthetic substrates. Evidence for different enzymes, including a true acetylcholinesterase and a pseudocholinesterase, has been reported previously (Leibel 1988). The poor BChE activity observed in the dab brain, together with its high activity in muscle and liver, support the hypothesis that butyrylthiocholine is hydrolysed by a n atypical cholinesterase (Toutant et al 1989) and acetylthiocholine by both a true cholinesterase and a pseudocholinesterase.…”

Section: Discussionmentioning

confidence: 99%

Evidence of variation in cholinesterase activity in fish along a pollution gradient in the North Sea

Galgani¹,

Bocquené²,

Cadiou³

1992

Mar. Ecol. Prog. Ser.

106

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Dunng the Bremerhaven Workshop chollnesterase measurements In d a bL~m a n d a llrnanda muscle were evaluated as a monitoring tool to assess the effect of pollutants along a 360 km transect In the North Sea, and around a dnlling s~t e The baslc properties of chol~nesterases, together wlth t h e~r natural vanabihty related to sex and size, were lnvestlgated The results show the presence of at least 2 d~fferent enzymes, acetylcholinesterase and butyrylchol~nesterase w t h h~g h actlv~tles In b r a n , muscle and l~v e r No varlatlon was observed In relation to sex or slze The act~vity of both acetylcholinesterase and butyrylcholinesterase was depressed In nearshore statlons along the transect and no variation was observed around the dr~lhng slte The K,, of acetylchol~nesterase from muscle of d a b varied along the transect The results lead us to Interpret enzyme var~ation as the result of effects of neurotoxic compounds coming from the Elbe and Weser rlvers into the German B~g h t and validate cholinesterase activlty as a tool for biological monltonng at sea

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“…In the flounder (P. flesus), the atypical AChE was largely predominant (92% of total AChE), which is consistent with our hypothesis of a predominant atypical AChE (83% of total AChE) in A. polyacanthus muscle tissue. In tropical species, an atypical ChE was described in the surgeonfish Acanthurus dussumieri, using enzyme kinetics as well as chromatography techniques to purify the enzyme (Leibel, 1988). Similarly, Leticia and Gerardo (2008) found a typical AChE and an atypical, BW284c51-sensitive BChE in muscle of the tropical reef fish Haemulon plumieri from the Yucatan peninsula.…”

Section: Che Characterizationmentioning

confidence: 96%

Effects of chlorpyrifos on cholinesterase activity and stress markers in the tropical reef fish Acanthochromis polyacanthus

Botté

Jerry

King

et al. 2012

Marine Pollution Bulletin

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An analysis of esterase activities from surgeonfish tissues yields evidence of an atypical pseudocholinesterase

Cited by 15 publications

References 36 publications

Different sensitivity to organophosphates of acetylcholinesterase and butyrylcholinesterase from three‐spined stickleback (Gasterosteus aculeatus): Application in biomonitoring

Different sensitivity to organophosphates of acetylcholinesterase and butyrylcholinesterase from three‐spined stickleback (Gasterosteus aculeatus): Application in biomonitoring

Evidence of variation in cholinesterase activity in fish along a pollution gradient in the North Sea

Effects of chlorpyrifos on cholinesterase activity and stress markers in the tropical reef fish Acanthochromis polyacanthus

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