Invalid PVT data provide high uncertainty in field development studies such as reservoir fluid compartmentalization, reserve estimation, reservoir simulation, production engineering, and surface facility design. Then, consistency check should be conducted to ensure the validity of PVT data and to identify the most representative PVT sample(s). In this paper, preliminary and complementary consistency check of PVT laboratory methods was applied to design a new comprehensive validity check procedure for verification of the reservoir fluid properties. This procedure would be ascertained whether or not the data could be used as the most representative fluid sample in further studies. The data used in this study were collected from seven undersaturated oil reservoirs, and challenges were observed through the 28 full set of available PVT laboratory data. In our study quality checking procedure of PVT samples consists of different methods such as graphical method, recombination and material balance check for well stream composition test, Buckley, modified Wilson and Hoffman plot, Watson characterization factor, Y-function of CCE test, Y-function of DL test, compositional material balance of DL test, overall mass balance for density, Bo and GOR of DL test and density check for separator test. After implementing the applicable proposed checking procedure to detect the validity of laboratory PVT sample, only 9 out of 28 PVT samples were satisfied all the preliminary and complementary check methods. Furthermore, a practical strategy, to select representative reservoir fluid sample, was also provided and discussed based on the consistency check, well condition check and comparison of fluid properties.