An Analytical Study of Population Growth in Drosophila melanogaster

Chiang, H. C.; Hodson, A. C.

doi:10.2307/1948580

Cited by 139 publications

(112 citation statements)

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“…1), and the oviposition and viability of flies (25 females and 20 males) were quantitatively investigated. When compared to conventional oviposition assays, our technique has provided a Surface quality, texture, and chemistry have been shown to have significant effects on flies' oviposition, 24,25,30,31,[33][34][35][36][37] however, the required area of exposure to agar substrate, in order to keep a population of fruit flies alive and to induce them to lay their eggs naturally (i.e., similarly to pure agar substrates) were not investigated. Accordingly, our aim was to quantitatively investigate the effects and the threshold values of surface exposure area on oviposition of fruit flies using our PDMS membrane patterning technique.…”

Section: Resultsmentioning

confidence: 99%

“…32 Current surface texture studies may be categorized into additive and subtractive texturing approaches. In the former method, seeded bisected grapes 31 or blotters, 33 silk meshes, 30 and different kinds of papers 30 (such as cardboard strips, filter paper, and Washi paper) on top of standard agar substrates have been used to produce different surface modifications. In the latter approaches, manually scratched agar plates 33,34 were used to produce texturized surfaces.…”

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confidence: 99%

“…It was shown that egg-laying was enhanced and more prominent on rough surfaces and on sharp edges produced by manual incisions of substrates with sharp objects to create slices, slits, and grooves. 17,[33][34][35] Atkinson showed that texture is important in oviposition when investigating the reason for gregarious ovipositional effects. 35 They created 12 egg-laying pots filled with yeast-agar and patterned half of them manually by scratching with a 5 mm deep perpendicular grid.…”

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Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

et al. 2015

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Drosophila melanogaster (fruit fly) is a model organism and its behaviours including oviposition (egg-laying) on agar substrates have been widely used for assessment of a variety of biological processes in flies. Physical and chemical properties of the substrate are the dominant factors affecting Drosophila's oviposition, but they have not been investigated precisely and parametrically with the existing manual approaches. As a result, many behavioral questions about Drosophila oviposition, such as the combined effects of the aforementioned substrate properties (e.g., exposure area, sugar content, and stiffness) on oviposition and viability, and their threshold values, are yet to be answered. In this paper, we have devised a simple, easily implementable, and novel methodology that allows for modification of physical and chemical composition of agar substrates in order to quantitatively study survival and oviposition of adult fruit flies in an accurate and repeatable manner. Agar substrates have been modified by surface patterning using single and hexagonally arrayed through-hole polydimethylsiloxane (PDMS) membranes with various diameters and interspacing, as well as by substrate stiffness and sugar content modification via alteration of chemical components. While pure PDMS substrates showed a significant lethal effect on flies, a 0.5 mm diameter through-hole access to agar was found to abruptly increase the survival of adult flies to more than 93%. Flies avoided ovipositing on pure PDMS and on top of substrates with 0.5 mm diameter agar exposure areas. At a hole diameter of 2 mm (i.e., 0.25% exposure area) or larger, eggs were observed to be laid predominately inside the through-holes and along the edges of the PDMS-agar interface, showing a trending increase in site selection with 4 mm (i.e., 1% exposure area threshold) demonstrating natural oviposition rates similar to pure agar. The surfacemodified agar-PDMS hybrid devices and the threshold values reported for the substrate physical and chemical conditions affecting oviposition are novel; therefore, we advocate their use for future in-depth studies of oviposition behaviour in Drosophila melanogaster with accuracy and repeatability. The technique is also useful for development of novel assays for learning and decision-making studies as well as miniaturized devices for self-assembly of eggs and embryonic developmental investigations. V C 2015 AIP Publishing LLC. [http://dx

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Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

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“…Indeed, some reports have indicated that oviposition is inhibited by extremely used food (Chess and Ringo, 1985;Chiang and Hodson, 1950), but this effect has yet to be investigated directly. Interestingly, interactions between different larval species (Budnik and Brncic, 1974;Budnik and Brncic, 1975;Hodge et al, 1999;Miller, 1964) and genotypes (Dawood and Strickberger, 1969;Lewontin, 1955;Saltz et al, 2012) can affect larval development and survival differently, and it would be interesting to see whether females can attend to and modulate their attraction to cues associated with different species and genotypes accordingly.…”

Section: Discussionmentioning

confidence: 99%

Adult fruit fly attraction to larvae biases experience and mediates social learning

Durisko

Anderson

Dukas

2013

Journal of Experimental Biology

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We investigated whether adult fruit flies (Drosophila melanogaster) use cues of larvae as social information in their food patch choice decisions. Adult male and female fruit flies showed attraction to odours emanating from foraging larvae, and females preferred to lay eggs on food patches occupied by larvae over similar unoccupied patches. Females learned and subsequently preferred to lay eggs at patches with novel flavours previously associated with feeding larvae over patches with novel flavours previously associated with no larvae. However, when we controlled for the duration of exposure to each flavoured patch, females no longer preferred the flavour previously associated with feeding larvae. This suggests that social learning in this context is indirect, as a result of strong social attraction biasing experience.

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“…There is evidence from both intraspecific and interspecific competition studies to indicate that relative pre-adult viabilities vary with larval density (Sang, 1949;Chiang and Hodson, 1950;Lewontin, 1955;and Barker and Podger, 1970), availability of food (Bakker, 1961) and the relative frequency of the competing species or genotypes (Barker and Podger, 1970;Putwain et aI., 1967). Additional evidence indicates that the fitness of a particular genotype in a given environment may be improved or impaired by the presence in that same environment of conspecific carriers of other genotypes (Levene et aI., 1954;Beard-Revised October 15, 1979 more, 1963).…”

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ALTERED COMPETITION BETWEEN TWO REPRODUCTIVELY ISOLATED STRAINS OFDROSOPHILA MELANOGASTER

Pruzan-Hotchkiss¹,

Perelle²,

Hotchkiss³

1980

Evolution

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An Analytical Study of Population Growth in Drosophila melanogaster

Cited by 139 publications

References 23 publications

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

Adult fruit fly attraction to larvae biases experience and mediates social learning

ALTERED COMPETITION BETWEEN TWO REPRODUCTIVELY ISOLATED STRAINS OFDROSOPHILA MELANOGASTER

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