An anatomical study of the microcirculation in the rabbit femur

Khan, Ralph A. A.

doi:10.1007/bf01915374

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1994

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“…Microfil (Flow Tek. Inc., Boulder, Colorado) is a silicone rubber injection compound developed and widely used for the morphological examination of tissue spaces (Khan, 1978;Lennon et al, 1991;Nelson et al, 1991). This casting material is popular in microvascular research.…”

Section: Introductionmentioning

confidence: 99%

Severe shrinkage of Microfil during tissue clearing with the Spalteholz technique

Møller

Robertsen

Hansen

1994

Journal of Microscopy

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Summary The effect of two commonly used tissue clearing methods on the morphology of vascular casts formed by Microfil, a silicone rubber injection compound, was investigated. Microfil undergoes extreme shrinkage when the casted tissue is cleared by the alchohol‐methyl salicylate clearing technique. No shrinkage is observed when the alternative glycerin clearing method is used. The alcohol‐methyl salicylate clearing technique should be avoided in studies employing Microfil.

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Section: Introductionmentioning

confidence: 99%