An antibody that confers plant disease resistance targets a membrane‐bound glyoxal oxidase in <i>Fusarium</i>

Song, Xiu-Shi; Xing, Shu; Li, Heping; Zhang, Jingbo; Jiang, Jingjing; Fan, Chao; Yang, Peng; Liu, Jinlong; Hu, Ziyi; Xue, Sheng; Liao, Yu-Cai

doi:10.1111/nph.13806

Cited by 25 publications

(21 citation statements)

References 56 publications

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“…Plant pathogenic fungi can cause a serious reduction in the crop yield and affect the quality of agricultural products [34,35,36,37]. Revealing the infection mechanisms of the plant pathogenic fungi can help us to develop novel strategies to control fungal diseases.…”

Section: Research Progress and Application Of Metabolomics In Fungmentioning

confidence: 99%

Advances of Metabolomics in Fungal Pathogen–Plant Interactions

Chen

2019

Metabolites

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Plant disease caused by fungus is one of the major threats to global food security, and understanding fungus–plant interactions is important for plant disease control. Research devoted to revealing the mechanisms of fungal pathogen–plant interactions has been conducted using genomics, transcriptomics, proteomics, and metabolomics. Metabolomics research based on mass spectrometric techniques is an important part of systems biology. In the past decade, the emerging field of metabolomics in plant pathogenic fungi has received wide attention. It not only provides a qualitative and quantitative approach for determining the pathogenesis of pathogenic fungi but also helps to elucidate the defense mechanisms of their host plants. This review focuses on the methods and progress of metabolomics research in fungal pathogen–plant interactions. In addition, the prospects and challenges of metabolomics research in plant pathogenic fungi and their hosts are addressed.

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Section: Research Progress and Application Of Metabolomics In Fungmentioning

confidence: 99%

Advances of Metabolomics in Fungal Pathogen–Plant Interactions

Chen

2019

Metabolites

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“…Another indication for a role of H 2 O 2 and H 2 O 2 -producing enzymes in pathogenicity is provided by two independent studies showing that Botrytis cinerea became less virulent upon deletion of genes encoding either superoxide dismutase (SOD) (230) or GLOX (231). Membrane-bound GLOX has also been implicated in pathogenesis for crop pest Fusarium species (232).…”

Section: Figmentioning

confidence: 99%

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Bissaro

Várnai

Røhr

et al. 2018

Microbiol Mol Biol Rev

240

246

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SUMMARYBiomass constitutes an appealing alternative to fossil resources for the production of materials and energy. The abundance and attractiveness of vegetal biomass come along with challenges pertaining to the intricacy of its structure, evolved during billions of years to face and resist abiotic and biotic attacks. To achieve the daunting goal of plant cell wall decomposition, microorganisms have developed many (enzymatic) strategies, from which we seek inspiration to develop biotechnological processes. A major breakthrough in the field has been the discovery of enzymes today known as lytic polysaccharide monooxygenases (LPMOs), which, by catalyzing the oxidative cleavage of recalcitrant polysaccharides, allow canonical hydrolytic enzymes to depolymerize the biomass more efficiently. Very recently, it has been shown that LPMOs are not classical monooxygenases in that they can also use hydrogen peroxide (H2O2) as an oxidant. This discovery calls for a revision of our understanding of how lignocellulolytic enzymes are connected since H2O2is produced and used by several of them. The first part of this review is dedicated to the LPMO paradigm, describing knowns, unknowns, and uncertainties. We then present different lignocellulolytic redox systems, enzymatic or not, that depend on fluxes of reactive oxygen species (ROS). Based on an assessment of these putatively interconnected systems, we suggest that fine-tuning of H2O2levels and proximity between sites of H2O2production and consumption are important for fungal biomass conversion. In the last part of this review, we discuss how our evolving understanding of redox processes involved in biomass depolymerization may translate into industrial applications.

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“…The central physiological function of GLOx remains unknown, but experimental reports reveal that the enzyme is important for hyphal tip development and pathogenicity [34,35]. Moreover, some GLOxes are linked to the cell Wall Stress-responsive Component (WSC) domain that is predicted to be a cell-wall and membrane protein in yeast and fungi associated with cell wall integrity and stress responses [36,37].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a New Glyoxal Oxidase from the Thermophilic Fungus Myceliophthora thermophila M77: Hydrogen Peroxide Production Retained in 5-Hydroxymethylfurfural Oxidation

et al. 2018

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Myceliophthora thermophyla is a thermophilic industrially relevant fungus that secretes an assortment of hydrolytic and oxidative enzymes for lignocellulose degradation. Among them is glyoxal oxidase (MtGLOx), an extracellular oxidoreductase that oxidizes several aldehydes and α-hydroxy carbonyl substrates coupled to the reduction of O2 to H2O2. This copper metalloprotein belongs to a class of enzymes called radical copper oxidases (CRO) and to the “auxiliary activities” subfamily AA5_1 that is based on the Carbohydrate-Active enZYmes (CAZy) database. Only a few members of this family have been characterized to date. Here, we report the recombinant production, characterization, and structure-function analysis of MtGLOx. Electron Paramagnetic Resonance (EPR) spectroscopy confirmed MtGLOx to be a radical-coupled copper complex and small angle X-ray scattering (SAXS) revealed an extended spatial arrangement of the catalytic and four N-terminal WSC domains. Furthermore, we demonstrate that methylglyoxal and 5-hydroxymethylfurfural (HMF), a fermentation inhibitor, are substrates for the enzyme.

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An antibody that confers plant disease resistance targets a membrane‐bound glyoxal oxidase in Fusarium

Cited by 25 publications

References 56 publications

Advances of Metabolomics in Fungal Pathogen–Plant Interactions

Advances of Metabolomics in Fungal Pathogen–Plant Interactions

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass

Characterization of a New Glyoxal Oxidase from the Thermophilic Fungus Myceliophthora thermophila M77: Hydrogen Peroxide Production Retained in 5-Hydroxymethylfurfural Oxidation

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