An Antibody Which Precipitates Dane Particles in Acute Hepatitis Type B: Relation to Receptor Sites Which Bind Polymerized Human Serum Albumin on Virus Particles

Abstract

An antibody, which is distinct from the HBsAg- reacts with antigenic sites on Dane particles- HBcAg and HBeAg, was studied by radioimmunoprecipitation of radioactive intact hepatitis B virions in sera obtained early in the course of acute hepatitis type B. The antibody, previously termed anti-Dane particle (anti-DP) antibody, was reactive with Dane particles and HBsAg particles obtained from HBeAg-positive sera but not with HBsAg particles from anti-HBe containing sera. The expression on virus particles of the evoking antigen correlated with levels of binding sites for polymerized human serum albumin (pHSA) as detected by solid-phase radioimmunoassay. In acute hepatitis B sera, levels of anti-DP antibody activity showed inverse correlation with expression of pHSA receptors on circulating virus particles, although the two reactivities were not mutually exclusive. In inhibition experiments, pHSA blocked precipitation of Dane particles by anti-DP positive sera, while native human albumin and polymerized bovine albumin had no effect. The inhibition by pHSA of the anti-DP reaction appeared specific since identical concentrations of pHSA did not interfere with precipitation of virus particles by anti-HBs. Affinity chromatography studies with anti-DP insolubilized on Sepharose 4B columns showed selective binding to the gel of radioactive Dane particles; 125I-HBsAg was not reactive. The binding of Dane particles to anti-DP columns was completely inhibited when virus particles were applied to the gel in pHSA; pretreatment of the column with pHSA did not affect the reaction.(ABSTRACT TRUNCATED AT 250 WORDS)

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

“…On the other hand, antibodies against the pHSA receptor which have been shown in serum during acute hepatitis B may have an important role for viral clearance (Alberti et al, 1984;Pontisso et al, 1983b). As a result, it would be useful to use HBsAg particles carrying the pHSA receptor in vaccination programs (Michel et al, 1984).…”

Evaluation of a new hepatitis B triple-antigen vaccine in inadequate responders to current vaccines

“…[11][12][13] Also, these domains stimulate cellular immune responses and, importantly in the context of this study, can help bypass genetic nonresponsiveness to the S antigen. [14][15][16][17][18][19][20][21] This novel vaccine has been developed to produce a superior immunologic response to that of currently approved vaccines. Animal studies have indicated that it was well tolerated, 6,7 and a viral challenge study in chimpanzees has shown protective efficacy.…”

Nucleotide Sequence of a Cloned Hepatitis B Virus Genome, Subtype ayr: Comparison with Genomes of the Other Three Subtypes

“…The product encoded by the pre-S region has attracted increasing attention, owing to its strong immunogenicity and association with the species-specific receptor for polymerized human and chimpanzee albumins Machida et al, 1984). Antibodies against this product may have clinical implications, because they could be responsible for the clearance of HBV from the circulation (Alberti et al, 1984). Furthermore, the pre-S(2) sequence is highly immunogenic in mice (Coursaget et al, 1985;Milich et al, 1985); it may add protective efficacy to a hepatitis B vaccine involving primarily the product of the S gene.…”