An antifungal protein from Escherichia coli

Yadav, Vipul; Mandhan, Rishi Pal; Pasha, Qadar; Pasha, Santosh; Katyal, Anju; Chhillar, Anil Kumar; Gupta, J. R. P.; Dabur, Rajesh; Sharma, Gainda L.

doi:10.1099/jmm.0.46973-0

Cited by 20 publications

(20 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein profile of the crude lysate gave large number of bands when run on SDS-PAGE and the fractified peak with the highest antifungal activity showed many protein bands in the molecular mass range of 20 -45 KDa. This result was in accordance with that observed by Yadav et al (2005a and2007) showing that the fractifaction of E. coli BL21 lysate showed 5 fractions of protein. The protein profile of crude E. coli BL21 lysate and the active fraction with antifungal activity showed that crude lysate gave various bands when run on SDS-PAGE, while the active fraction with antifungal activity demonstrate two major bands in the molecular mass range of 29 to 43 KDa.…”

Section: Discussionsupporting

confidence: 82%

“…In the present study, crude lysate was non toxic up to a test concentration of 200 µg/ml on the other hand, the fractified fraction with antifungal activity FΙ showed no toxicity with all tested concentrations up to 2000 µg/ml against erythrocytes, as compared to this results with that obtained by Yadav et al (2005a and2007) who showed that E. coli BL21 total lysate and completely purified fraction with antifungal activity PPEBL21 were non toxic up to a test concentration of 1250 µg/ml to human erythrocytes. These results gave evidence that the partially purified L. fermentum lysate is totally safe for producing potent antifungal compound.…”

Section: Discussionsupporting

confidence: 60%

“…They revealed that E. coli reported a very important medicinal value which was also supported by Matricardi et al (2003). The supernatant of Streptomyces thermonitrificans showed better anti-Aspergillus activity (MIC, 62.5 µg/ml) than lysate (MIC, 125 µg/ml) Yadav et al (2005aYadav et al ( , 2007. C. albicans remains the most susceptible of the yeasts studied for fluconazole and itraconazole when compared with non C. albicans (Panizo et al, 2009).…”

Section: Discussionmentioning

confidence: 89%

See 2 more Smart Citations

Medical importance of Lactobacillus fermentum lysate as a bioactive agent against some pathogenic Candida and Aspergillus strains

Abedin¹,

El-Aassar²,

Bahloul³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

In search of a safe and new biosource for treating fungal infections, the bacterial lysate of probiotic Lactobacillus fermentum was tested for its antifungal activity against Aspergillus niger, Aspergillus flavus and Candida albicans. The antifungal activity of lysate was analyzed by dry weight, disc diffusion and micro broth dilution methods. The growth of A. niger and A. flavus was inhibited by 31 µg lysate protein/disc but C. albicans was firstly inhibited at 62 µg lysate protein/disc. MIC for inhibition of A. niger was recorded as 125 µg lysate/ml while, A. flavus and C. albicans were inhibited at MIC of 62 µg lysate/ml by micro broth dilution assay. De Man Rogose and Sharpe medium supplemented with wheat bran, corn steep liquer and yeast extract showed the highest yield, 10 g dry biomass/L of L.fermentum at pH 6.8. The elution profile of the purified lysate showed five fractions, the first gave more than 60% of the original sample. Its MIC was 25 µg lysate/disc. Its molecular weight was 20 to 30 KDa. Toxicity tests revealed that, up to 150 µg lysate/ml showed no significant haemolysis.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Medical importance of Lactobacillus fermentum lysate as a bioactive agent against some pathogenic Candida and Aspergillus strains

Abedin¹,

El-Aassar²,

Bahloul³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Dissimilarly, many authors worked on using B. pumilus in order to obtain bioactive secondary metabolites acting against different pathogenic fungi regardless the biotoxicity of these metabolites towards human or plant (Ghasemi et al, 2012;Bullerman, 1997, 1998;Munimbazi and Bullerman, 1998). On the other hand, Yadav et al (2007) purified a cytosolic protein from E. coli BL21; it demonstrated potent antifungal activity against pathogenic strains of Aspergillus species (A. fumigatus, A. flavus, A. niger) and C. albicans with MIC of 1.95 to 3.98 and 15.62 mg ml -1 , respectively, and it showed no cytotoxicity up to 1250 mg ml -1 in vitro toxicity tests, which is very high concentration compared to the LC 50 of the antifungal agent of B. pumilusSMH101 (Table 4).…”

Section: Types Of Antifungal Agentmentioning

confidence: 99%

Antifungal agent production from a new marine Bacillus pumilusSMH101

El-Naggar¹,

Ibrahim²,

Battah³

et al. 2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Twenty two (22) marine bacterial strains were isolated and tested to inhibit some plant and human pathogenic fungi; Fusarium solani, Rhizoctonia solani, Aspergillus niger, Fusarium exosporium and Candida albicans. The most potent marine bacterium was identified as Bacillus pumilusSMH101 on using 16S rRNA. The peptone water medium (PW) showed maximum antifungal activity. The PlacketBurman experimental design was applied and the optimum culture conditions were inoculum size (8.0 × 10 6 cfu/ml), temperature (25°C), incubation period (24 h) and pH value of 7.0. The trickle flow column was tested for propagating the antifungal production using luffa pulp and synthetic sponge as solid packing materials. The adsorbed B. pumilusSMH101 showed lower average fungal suppression (~ 40%) compared to the free bacterial cells (58.6%). Thin layer chromatography (TLC) was applied using a solvent system of dichloromethane: methanol: water (65:32:3 v/v). A single ultraviolet (UV) spot was obtained with a retardation factor (R f ) of 0.75. It analyzed using UV, infrared (IR) and mass spectrometry (MS) spectra and showed a molecular weight of 875 Da. Also, it showed a relatively low bio-toxicity (LC 50 = 1072 ppm) and a broad antifungal spectrum with a bioactivity of 87, 80 and 70% against F. solani, R. solani and C. albicans, respectively, compared to some commercial antifungal drugs based on the active fluconazole compound which applied in a concentration of tenfold more than the used B. pumilusSMH101 antifungal agent concentration (0.05 mg/ml).

show abstract

“…Yadav et al (2010Yadav et al ( , 2007 reported that cytosolic proteins of E. coli are responsible for antifungal potential against pathogenic strains of Aspergillus fumigatus, Aspergillus flavus, A. niger and Candida albicans. Also, Mushtaq et al (2010) reported that E. coli showed almost complete inhibition against pathogenic fungi such as A. niger and A. flavus.…”

Section: Staphylococcus Aureus (Mrsa) E Coli M2 Effectivelymentioning

confidence: 99%

Molecular characterization of bacteria isolated from the Kingdom of Saudi Arabia and their uses against pathogenic fungi causing dermatological diseases

Mohammed¹,

Sheikh²

2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Random amplified polymorphic DNA (RAPD) assay and antimicrobial activities were used for the detection of genetic variations of antimicrobial isolates from the Kingdom Saudi of Arabia. Soil samples were collected from El-Madina El-Monawara, El-Taif, El-Jouf and El-Dammam for bacterial isolation. Bacterial isolates were evaluated for their antimicrobial activities against pathogenic fungi which cause dermatological diseases; Acremonium sp., Microsporium gypseum, Microsporium gallinae, Exserohilum sp., Trichphyton sp. and Aspergillus niger. Results indicate that all the bacterial isolates showed different levels of antagonistic activities against the pathogenic fungi. Esherichia coli M2, Bacillus megaterium M3 and Bacillus subtilis M10 showed strong effect against all the tested pathogenic fungi. B. subtilis J1 isolate showed strong effect against the two pathogenic fungi M. gallinae and Exserohilum sp. Nine strains were tested for their antibiotic response. The results show that different antibiotic response was found. RAPD fingerprinting technique was applied to study the genetic diversity among the tested strains. Results indicate that there was a significant genomic diversity among the B. subtilis strains and this diversity was highly correlated with habitats and the antagonistic effect of the studied strains.

show abstract

An antifungal protein from Escherichia coli

Cited by 20 publications

References 36 publications

Medical importance of Lactobacillus fermentum lysate as a bioactive agent against some pathogenic Candida and Aspergillus strains

Medical importance of Lactobacillus fermentum lysate as a bioactive agent against some pathogenic Candida and Aspergillus strains

Antifungal agent production from a new marine Bacillus pumilusSMH101

Molecular characterization of bacteria isolated from the Kingdom of Saudi Arabia and their uses against pathogenic fungi causing dermatological diseases

Contact Info

Product

Resources

About