1993
DOI: 10.1055/s-0038-1651586
|View full text |Cite
|
Sign up to set email alerts
|

An Antithrombin III Assay Based on Factor Xa Inhibition Provides a More Reliable Test to Identify Congenital Antithrombin III Deficiency Than an Assay Based on Thrombin Inhibition

Abstract: SummaryObjectives: To determine whether functional antithrombin III (AT-III) levels measured by a factor Xa inhibition (AT-III-Xa) assay identifies AT-III deficient individuals more reliably than functional AT-III levels measured by a thrombin inhibition (AT-III-IIa) assay.Study design: Cross-sectional study.Patient population: Sixty-seven members of a large family with type 2 AT-III deficiency.Intervention: DNA analysis was used as the reference diagnostic standard for AT-III status and subjects were classifi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

4
36
0

Year Published

1994
1994
2013
2013

Publication Types

Select...
7
2

Relationship

0
9

Authors

Journals

citations
Cited by 53 publications
(40 citation statements)
references
References 16 publications
4
36
0
Order By: Relevance
“…This method is routinely used in clinical practice, because it is the most reliable test to identify congenital antithrombin deficiency. 17 The prevalence of the classic prothrombotic polymorphisms was similar to that described in other series. Thus, the FV Leiden was present in 145 patients (3 in homozygous state) (14.2%), and the prothrombin 20210A allele was identified in 101 patients (7 in homozygous state) (9.9%) ( Table 1).…”
Section: Case-control Studysupporting
confidence: 85%
See 1 more Smart Citation
“…This method is routinely used in clinical practice, because it is the most reliable test to identify congenital antithrombin deficiency. 17 The prevalence of the classic prothrombotic polymorphisms was similar to that described in other series. Thus, the FV Leiden was present in 145 patients (3 in homozygous state) (14.2%), and the prothrombin 20210A allele was identified in 101 patients (7 in homozygous state) (9.9%) ( Table 1).…”
Section: Case-control Studysupporting
confidence: 85%
“…Moreover, 62 carriers, including 4 homozygous subjects (from the studies of Tait et al, 13 Perry et al, 21 and this study) have normal plasma levels of antithrombin antigen (99.5% Ϯ 13.1%). Because these are the methods commonly used to identify antithrombin deficiency, 17,22 we can understand why this mutation has not been identified in other populations, and, probably, its prevalence had been underestimated. Moreover, this variant only slightly impaired the anti-IIa activity (74.3% Ϯ 8.5%) of 60 carriers (from the studies of Tait et al, 13 Perry et al, 21 and this study).…”
Section: Discussionmentioning
confidence: 99%
“…Human thrombin also reacted with heparin cofactor II and made the assay relatively insensitive for the detection of AT deficiency (89,90). In most commercial kits, human thrombin has been replaced by bovine thrombin which shows minimal reaction with heparin cofactor II.…”
Section: Laboratory Diagnosis Of Antithrombin Deficiencymentioning
confidence: 99%
“…Total protein S antigen, 1 free protein S antigen 15 and functional protein S activity 16 were measured according to the manufacturer's instructions (Roche diagnostics, Tokyo). Antithrombin III activity, 18 functional protein C activity 19 and protein C antigen were measured according to the manufacturer's instructions (Daiiti-kagakuyakuhin, Sismex, and Iatron Laboratories, Inc, respectively). Although the total amount of protein S antigen was 65% (at around the lower limit of normal), the level of free protein S antigen was significantly low (31%).…”
mentioning
confidence: 99%