An apparatus and a method using phenethylamine for liquid scintillation counting of C14O2 obtained by wet oxidation of biological materials

Effect of cholestyramine on the fecal excretion of intravenously administered cholesterol-4-14C and its degradation products in a hypercholesterolemic patient

“…Duplicate aliquots of these fractions and the homogenized stool samples were used to measure 14C radioactivity. The carbon was converted to CO2 by the wetcarbon combustion method of Van Slyke, Folch, Plazin, and Weisiger (23,24), collected in a reservoir, and absorbed into phenethylamine by a method devised by the authors (25); the 14C was measured in a liquid scintillation counter with a solution of 0.3% diphenyloxazole in toluene containing the phenethylamine and methanol. (A solution containing 2 ml of 1: 1 phenethylamine: methanol and 10 ml of 0.3% diphenyloxazole was adequate for up to 6 mmoles of carbon dioxide.…”