An Apparent Rachitogenic Effect of Excessive Vitamin E Intakes in the Chick

Murphy, Timothy P.; Wright, Kenneth E.; Pudelkiewicz, W. J.

doi:10.3382/ps.0601873

Cited by 25 publications

(16 citation statements)

References 21 publications

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“…At Day 14, however,  and -tocopherol showed no effect on formation of the calcified nodules. These findings also indicate the inhibition of the differentiation to the osteoblasts producing osteoid, but not of the differentiation to the osteoblasts producing the calci- observed that excessive vitamin E supplementation reduced calcium and phosphorus in bone in chick [25]. From our result, it seems to be possible that the decrease of the amount of calcified bone matrix by vitamin E supplementation in chick was caused by inhibition of osteoid formation.…”

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confidence: 57%

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Effects of Vitamin E on the Osteoblast Differentiation

et al. 2010

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ABSTRACT. Vitamin E is thought to affect bone formation and bone remodeling. In this study, we investigated the effects of vitamin E (-tocopherol and -tocopherol) on the osteoblasts isolated from rat calvariae. At 4 and 7 days (Day 4 and 7) after induction of osteoblastic differentiation, treatment of -tocopherol (100 and 200 M) and -tocopherol (2 and 20 M) for 3 days significantly decreased alkaline phophatase activity of the cultured osteoblasts. At Day 14, however, no significant change was detected in ALP activity and expression of bone sialoprotein mRNA in the osteoblasts treated with -tocopherol or -tocopherol for 3 days. Expression of osteocalcin mRNA was decreased by treatment of -tocopherol (100 and 200 M) and -tocopherol (2 and 20 M) at Day 4 and 7. At Day 14, expression of osteocalcin mRNA was decreased only with treatment of 200 M -tocopherol. In addition, the noncalcified nodules were decreased by treatment of -tocopherol (200 M) and -tocopherol (20 M) at Day 7. However, treatment of -tocopherol and -tocopherol showed no significant change of formation of calcified nodules at Day 14. These results indicate that vitamin E inhibits differentiation of osteoblasts especially from early stage to osteoid-producing stage.KEY WORDS: alkaline phosphatase, cell culture, osteoblast, osteocalcin, tocopherol.J. Vet. Med. Sci. 72 (7): [951][952][953][954][955][956][957] 2010 Vitamin E (tocopherols and toctrienols) is an important cellular antioxidant that participates in several reactions to prevent peroxidation of membrane-bound lipids. Recently, many non-antioxidant functions of -tocopherol, a major vitamin E isoform, were described at cellular level, such as inhibition of proteinkinase C, inhibition of the smooth muscle cell proliferation and the activation of diacylglycerol kinase [3,4,[7][8][9]. In the skeletal system, several reports demonstrated effects of vitamin E on bone and cartilage tissues [1,20,28,32]. Ebina et al. reported that Fe-induced impairment of bone formation was prevented by dietary vitamin E supplementation in rats [13]. In addition, vitamin E stimulated trabecular bone formation in chicks [25]. Therefore, vitamin E is thought to affect bone formation and bone remodeling. However, direct effects of vitamin E on bone tissue at cellular level are still remained unclear. Among tocopherol isoforms, -tocopherol shows the most biologically active and is selectively retained in the body [2,15], and -tocopherol exhibits the most inhibitory effect to cell proliferation in several cell-types [23,24]. In this study, to test the influence of vitamin E on differentiation of osteoblasts at several stages of osteogenesis, we investigated change of alkaline phosphatase activity (ALP), and expression of osteocalcin and bone sialoprotein (BSP) as a marker of maturation of osteoblasts [17,27,31] in cultured osteoblasts by treatment with  and -tocopherol.Osteoblasts were isolated from calvariae of newborn rats by collagenase digestion according to the method described by Spector e...

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confidence: 57%

“…Ebina et al reported that Fe-induced impairment of bone formation was prevented by dietary vitamin E supplementation in rats [13]. In addition, vitamin E stimulated trabecular bone formation in chicks [25]. Therefore, vitamin E is thought to affect bone formation and bone remodeling.…”

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confidence: 99%

Effects of Vitamin E on the Osteoblast Differentiation

et al. 2010

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“…However, there is evidence that massive dietary doses of this vitamin may cause various disorders (March et al, 1973;Murphy et al, 1981;Aburto and Britton, 1996). An adverse effect of excessive intake of vitamin E is its interference with cholecalciferol utilisation, particularly when the latter's dietary concentration is marginal.…”

Section: Introductionmentioning

confidence: 99%

“…An adverse effect of excessive intake of vitamin E is its interference with cholecalciferol utilisation, particularly when the latter's dietary concentration is marginal. This interference is manifested mainly by reduction in bone ash and body weight (March et al, 1973;Murphy et al, 1981;Aburto and Britton, 1996).…”

Section: Introductionmentioning

confidence: 99%

Moderate excess of dietary vitamin E does not exacerbate cholecalciferol deficiency in young broiler chicks

Bartov¹

1997

British Poultry Science

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1. The combined effect of moderate excess dietary vitamin E and marginal amounts of dietary cholecalciferol on the performance and tibia bone ash of young male broiler chicks was evaluated. Vitamin E (alpha-tocopheryl acetate) and cholecalciferol were added to a commercial diet not already supplemented with these vitamins, at concentrations of 0 and 150 mg/kg, and 1.875, 5 and 25 micrograms/kg, respectively, and fed to chicks for 23 d. 2. Vitamin E concentration and its combinations with cholecalciferol did not significantly (P > 0.05) affect food intake, weight gain, food efficiency and bone ash. These variables were significantly (P < 0.001) lower in chicks fed on the diets supplemented with 1.875 micrograms cholecalciferol/kg compared with the values observed with the 2 other concentrations of this vitamin. There were no differences in the effects of 5 and 25 micrograms cholecalciferol/kg diet on the above variables. 3. It was concluded that vitamin E, at a concentration of 150 mg/kg diet, did not aggravate a mild cholecalciferol deficiency.

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“…This is possibly due to the interaction of vitamin E with vitamin D. Excess of VE in the diet can reduce Ca and P in the blood and bone (Murphy et al, 1981), absorption and utilization of vitamin A and D 3 (Leeson & Summers, 2001). In this case the vitamin D is dissolved in the unhydrolyzed and possibly nonsolubilized tocopheryl acetate and passes through the gut unabsorbed, consequently impaired bone and shell calcification.…”

Section: Physical Quality Of Eggmentioning

confidence: 99%

Supplementation of Mangosteen Pericarp Meal and Vitamin E on Egg Quality and Blood Profile of Laying Hens

Rusli¹,

Wiryawan

Toharmat

et al. 2015

Med Pet

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This research aimed to study the supplementation effects of mangosteen pericarp meal (MPM) and vitamin E (VE) in the diets on the egg quality and blood profile of laying hens. This research used 160 laying hens of Lohman strains 24 weeks of age. The observation was conducted for 11 weeks. A completely randomized design with four treatments and four replications (10 birds each) was used in this experiment. The treatments consisted of R0 (control diet), R1 (R0 + 1 g MPM/kg ration), R2 (R0 + 2 g MPM/kg ration) and R3 (R0 + 200 mg VE/kg ration). Variables measured were egg quality, yolk cholesterol, and blood profiles. The data were analyzed by using analysis of variance (ANOVA) and any significant difference between the treatment means were further tested by Duncan's Multiple Range Test. The results showed that supplementation of 1 g MPM/kg ration in the diet significantly (P<0.05) decreased blood triglycerides compared with the control, laying hens fed with diet suplemented with of 2 g MPM/kg ration, and laying hens with diet suplemented with 200 mg VE/kg ration. Supplementation of MPM and VE did not affect (P>0.05) egg quality (except shell thickness), blood cholesterol, and HDL, respectively. In conclusion, supplementation of 1 g MPM/kg in the diet of laying hens could decrease blood triglycerides.

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An Apparent Rachitogenic Effect of Excessive Vitamin E Intakes in the Chick

Cited by 25 publications

References 21 publications

Effects of Vitamin E on the Osteoblast Differentiation

Effects of Vitamin E on the Osteoblast Differentiation

Moderate excess of dietary vitamin E does not exacerbate cholecalciferol deficiency in young broiler chicks

Supplementation of Mangosteen Pericarp Meal and Vitamin E on Egg Quality and Blood Profile of Laying Hens

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