Ultrasonic (US) and UV-C disinfection technologies have been successfully used in wastewater treatment plants (WWTPs) for disinfection purposes. The US technology is typically used as a pre-treatment step to break down larger particles and make them more susceptible to disinfection. The UV-C technology is commonly used as a final disinfection step in many WWTPs. The study aimed to assess the potential of using Zinc Oxide (ZnO) Nanoparticles (NPs) to improve the effectiveness of UV-C and US disinfection methods in treating wastewater effluent, offering a more comprehensive solution to wastewater treatment. In this experimental study, a Laboratory US Bath (40[Formula: see text]kHz) and a UV-C lamp (16[Formula: see text]W) were used. In order to investigate the effectiveness of ZnO NPs in the reduction of microbial load, 5[Formula: see text]mg/L of ZnO NPs was added to the effluent samples. Then, samples were examined for Total Coliform (TC) and Fecal Coliform (FC) reduction by the standard MPN/100[Formula: see text]mL test. The Chick‘s law was used to calculate the efficiency of microbial load. The relationship between variables was determined by regression analysis using Excel and SPSS-ver 21 software. In this study, the samples were examined in three groups: Samples that were only exposed to sonication or received UV-C radiation with Turbidity of 18 NTU (Group A) and Turbidity of 5 NTU (Group B), and Samples that received 5[Formula: see text]mg/L of ZnO NPs (Group C). By increasing the time from 0.5[Formula: see text]min to 10[Formula: see text]min in the presence of UV-C, the amount of microbial population decreased, and 2[Formula: see text]min was considered the optimal time. The maximum removal efficiencies by US for TC were 74.07,77.7, 85.1% (40[Formula: see text]C) and 92.5,100, and 100% (60[Formula: see text]C) in group A (in 30[Formula: see text]min sonication), 85.7, 85.7, 100% (40[Formula: see text]C), respectively, and were 100% in other groups (B and C), respectively. The maximum removal efficiencies by US for FC were 76.4%, 88.2%, and 100% (40[Formula: see text]C) and 88.2%, 100%, and 100% (60[Formula: see text]C) in group A (in 30[Formula: see text]min sonication), respectively, and were 100% in other groups (B and C). In this study, an important increase in the disinfection ability of ZnO NPs has been observed in the presence of US and UV-C. So, the ZnO NPs/UV-C and ZnO NPs/US processes are valuable alternatives to conventional disinfection processes by over 90% improvement of disinfection efficiency.