An appraisal of polystyrene- (ELISA) and nitrocellulose-based (ELIFA) enzyme immunoassay systems using monoclonal antibodies reactive toward antigenically distinct forms of human C-reactive protein

Shields, Michael J.; Siegel, Joan N.; Clark, Carl R.; Hines, Kimberly; Potempa, Lawrence A.; Gewürz, Henry; Anderson, Byron

doi:10.1016/0022-1759(91)90152-6

Cited by 29 publications

(14 citation statements)

References 26 publications

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“…Residual sera from the hormonal assays were used to perform the initial CRP tests. An enzyme-linked immunoflow assay (ELIFA) was performed, with a minimum detection limit of 1 mg/l, in the Department of Cell, Molecular, and Structural Biology at NWU [30]. The second set of female cohort sera was derived from 21 pre-RA and 84 CN subjects, who constituted the remaining women eligible for inclusion in study and who had available stored (-70°C) sera in CLUE I (fig.…”

Section: Methodsmentioning

confidence: 99%

Serum Acute Phase Protein and Inflammatory Cytokine Network Correlations: Comparison of a Pre-Rheumatoid Arthritis and Non-Rheumatoid Arthritis Community Cohort

et al. 2013

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Serum concentrations of acute phase proteins, inflammatory cytokines, and other immunological components were individually assayed using high-sensitivity ELISA in a com-munity-based cohort of preclinical rheumatoid arthritis (pre-RA) and matched non-RA control (CN) subjects. Bivariate correlations of the biomarker panel concentrations were compared in pre-RA versus CN and female versus male subjects. Clinically elevated CRP levels (8+ mg/l) occurred in a higher (p = 0.010) frequency in 46 pre-RA (n = 8, 17.4%) subjects than in 179 CN (n = 9, 5.0%), and were independent of age, gender, smoking behaviors, and serum rheumatoid factor. Selected age and gender differences were found in levels of the immunological network factors. In each study group, the ratio of sTNF-RI to IL-2sRα mean concentrations was 2-fold higher in men than in women. Aging correlated positively with CRP, ASAA, and TNF-α levels, but negatively with IL-1β. Bivariate correlations were similar in pre-RA subjects versus CN and by gender, with few exceptions. Factor loadings in principal component analysis of the total subjects indicated that age- and gender-related variables constituted the two main components. Using multiple regression analyses, an integrative working model of all variable interrelations was generated. The tentative, directional model supports a concept of gender dimorphism of the ratio of sTNF-RI to IL-2sRα serum concentrations and displays differing effects of age on TNF-α versus IL-1β levels. These findings indicate complex age, gender, and cytokine interrelations in control of the immune systems network. Future research in testing such inflammatory pathways promises a better understanding of predisposition to diseases, like RA.

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Section: Methodsmentioning

confidence: 99%

Serum Acute Phase Protein and Inflammatory Cytokine Network Correlations: Comparison of a Pre-Rheumatoid Arthritis and Non-Rheumatoid Arthritis Community Cohort

et al. 2013

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“…(b) Amino acid composition analysis (Analytical Biotechnology Services, Boston, MA) showed that r m CRP contained the same number of amino acid residues per mol of protein as mCRP with the exception of 11 alanine residues per mol of r m CRP compared with 9 per mol of mCRP, no cysteine residues in r m CRP versus 2 cysteine residues per mol of mCRP and 3 methionine residues per mol of r m CRP compared with 2 in mCRP, corroborating the changes engineered in r m CRP. (20) react with the same specificity and affinity to mCRP, r m CRP (33), and acylated forms of r m CRP. Furthermore, preliminary experiments showed that, on a molar basis, mCRP and r m CRP and acylated forms of r m CRP produced similar delays in neutrophil apoptosis assessed by annexin V and acridine orange staining (see below).…”

mentioning

confidence: 99%

Loss of Pentameric Symmetry of C-reactive Protein Is Associated with Delayed Apoptosis of Human Neutrophils

Khreiss

József

Hossain

et al. 2002

Journal of Biological Chemistry

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148

164

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Human neutrophil granulocytes die rapidly, and their survival is contingent upon rescue from programmed cell death by signals from the environment. Here we report that a novel signal for delaying neutrophil apoptosis is the classic acute phase reactant, C-reactive protein (CRP). However, this anti-apoptotic activity is expressed only when the cyclic pentameric structure of CRP is lost, resulting in formation of modified or monomeric CRP (mCRP), which may be formed in inflamed tissues. By contrast, native pentameric CRP and CRP peptides 77-82, 174 -185, and 201-206 failed to affect neutrophil apoptosis. The apoptosis delaying action of mCRP was markedly attenuated by an antibody against the low affinity IgG immune complex receptor (CD16) but not by an anti-CD32 antibody. mCRP evoked a transient concurrent activation of the extracellular signalregulated kinase (ERK) and phosphatidylinositol 3-kinase/Akt signaling pathways, leading to inhibition of caspase-3 and consequently to delaying apoptosis. Consistently, pharmacological inhibition of either ERK or Akt reversed the anti-apoptotic action of mCRP; however, they did not produce additive inhibition. Thus, mCRP, but not pentameric CRP or peptides derived from CRP, promotes neutrophil survival and may therefore contribute to amplification of the inflammatory response.

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“…When proteins are adsorbed on material surfaces, some epitopes are masked, which reduces the binding of the corresponding antibodies [77][78][79]. Conversely, some internal protein epitopes may become exposed upon adsorption onto material surfaces, which increases the binding of the corresponding antibodies [80]. Monoclonal antibodies are therefore useful to determine the domain of interaction between proteins and material surfaces and to estimate the degree of unfolding of adsorbed proteins.…”

Section: Evidences Of Material-induced Protein Conformational Changesmentioning

confidence: 99%

Protein conformational changes induced by adsorption onto material surfaces: an important issue for biomedical applications of material science

Ballet¹,

Boulangé²,

Bréchet³

et al. 2010

Bulletin of the Polish Academy of Sciences: Technical Sciences

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Abstract. Protein adsorption on solid surfaces is a widespread phenomenon of large biological and biotechnological significance. Conformational changes are likely to accompany protein adsorption, but are difficult to evidence directly. Nevertheless they have important consequences, since the partial unfolding of protein domains can expose hitherto hidden amino acids. This remodeling of the protein surface can trigger the activation of molecular complexes such as the blood coagulation cascade or the innate immune complement system. In the case of extracellular matrix, it can also change the way cells interact with the material surfaces and result in modified cell behavior. In this review, we present direct and indirect evidences that support the view that some proteins change their conformation upon adsorption. We also show that both physical and chemical methods are needed to study the extent and kinetics of protein conformational changes. In particular, AFM techniques and cryo-electron microscopy provide useful and complementary information. We then review the chemical and topological features of both proteins and material surfaces in relation with protein adsorption. Mutating key amino acids in proteins changes their stability and this is related to material-induced conformational changes, as shown for instance with insulin. In addition, combinatorial methods should provide valuable information about peptide or antibody adsorption on well-defined material surfaces. These techniques could be combined with molecular modeling methods to decipher the rules governing conformational changes associated with protein adsorption.

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An appraisal of polystyrene- (ELISA) and nitrocellulose-based (ELIFA) enzyme immunoassay systems using monoclonal antibodies reactive toward antigenically distinct forms of human C-reactive protein

Cited by 29 publications

References 26 publications

Serum Acute Phase Protein and Inflammatory Cytokine Network Correlations: Comparison of a Pre-Rheumatoid Arthritis and Non-Rheumatoid Arthritis Community Cohort

Serum Acute Phase Protein and Inflammatory Cytokine Network Correlations: Comparison of a Pre-Rheumatoid Arthritis and Non-Rheumatoid Arthritis Community Cohort

Loss of Pentameric Symmetry of C-reactive Protein Is Associated with Delayed Apoptosis of Human Neutrophils

Protein conformational changes induced by adsorption onto material surfaces: an important issue for biomedical applications of material science

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