An approach for simultaneous determination for geographical origins of Korean Panax ginseng by UPLC-QTOF/MS coupled with OPLS-DA models

Abstract

Identification of the origins of Panax ginseng has been issued in Korea scientifically and economically. We describe a metabolomics approach used for discrimination and prediction of ginseng roots from different origins in Korea. The fresh ginseng roots from six ginseng cooperative associations (Gangwon, Gaeseong, Punggi, Chungbuk, Jeonbuk, and Anseong) were analyzed by UPLC-MS-based approach combined with orthogonal projections to latent structure-discriminant analysis multivariate analysis. The ginsengs from Gangwon and Gaeseong were easily differentiated. We further analyzed the metabolomics results in subgroups. Punggi, Chungbuk, Jeonbuk, and Anseong ginseng could be easily differentiated by the first two orthogonal components. As a validation of the discrimination model, we performed blind prediction tests of sample origins using an external test set. Our model predicted their geographical origins as 99.7% probability. The robust discriminatory power and statistical validity of our method suggest its general applicability for determining the origins of P. ginseng samples.

Metabolite Profiling of Justicia gendarussa Burm. f. Leaves Using UPLC-UHR-QTOF-MS

“…These factors may affect secondary metabolites qualitatively and quantitatively, so their bioactivities could be varied [21][22][23]. Therefore, metabolite profiling studies of herbal plants is very important for ensuring their safety and efficacy.…”

Investigating volatile compounds’ contributions to the stale odour of green tea

“…And the permutation test demonstrated that the OPLS‐DA model was reliable because the slope of both R2 and Q2 regression curve was almost near 1 (Fig. b; Song et al ., ; Triba et al ., ).…”

“…And the permutation test demonstrated that the OPLS-DA model was reliable because the slope of both R2 and Q2 regression curve was almost near 1 ( Fig. 3b; Song et al, 2013;Triba et al, 2015). Figure 3c shows the corresponding loading plot of the tea samples using the volatile compounds as variable.…”

Analysis of polyphenolic metabolites from Artemisia gmelinii Weber ex Stechm. and regional comparison in Korea

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