An approach to zwitterionic peptide design for colorimetric detection of the Southampton norovirus SV3CP protease

Yeung, Justin; Jin, Zhicheng; Ling, Chuxuan; Retout, Maurice; Barbosa da Silva, Elany; Damani, Manan; Chang, Yu-Ci; Yim, Wonjun; O'Donoghue, Anthony J.; Jokerst, Jesse V.

doi:10.1039/d3an00873h

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…BSPP-AgNPs with an average size of 80 nm were prepared from 80 nm citrate-AgNPs purchased from nanoComposix (San Diego, USA) through a modified ligand exchange procedure reported in a prior study. 34 This was done by adding 1 mL of 10 mM BSPP to 10 mL of the citrate-AgNPs. The resulting mixture was stirred for 18 h at 700 rpm.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Silver Nanoparticle Sensor Array for the Detection of SARS-CoV-2

Lam,

Retout,

Clark

et al. 2024

ACS Appl. Nano Mater.

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We report a silver nanoparticle (AgNP) sensor array to detect SARS-CoV-2 viral particles utilizing five peptide receptors to produce a colorimetric response. We show that the interaction of the virus particles with the peptides interferes with the shift in plasmonic absorbance and hinders the formation of snowflake-like fractal assemblies between the AgNPs and the bridging peptides. The limit of detection in water of the sensor array was 500,000 viral copies/mL. We demonstrate the capabilities of the sensor array to distinguish between SARS-CoV-2 (Beta, Delta, and Omicron variants) from the influenza virus at the 99% confidence level through linear discriminant analysis. The sensor array was also able to discriminate three out of five negative exhaled breath condensate samples from five positive SARS-CoV-2 EBC samples.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Silver Nanoparticle Sensor Array for the Detection of SARS-CoV-2

Lam,

Retout,

Clark

et al. 2024

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The defined readout time may vary depending on the sensors' sensitivity and selectivity toward target analytes and the types of analytes. 16,134 Given that colorimetric sensing is closely correlated with optical properties, spectrophotometry serves as a fundamental method for quantifying optical signal readouts. Optical signals can be defined as wavelength shift, intensity change, or color pattern evolution over time.…”

Section: System Readoutmentioning

confidence: 99%

“…This universal design can target other proteases by tuning the recognition sequence, and an example includes the Southampton norovirus 3C-like protease (SV3CP). 134 The charge-reversible substrate design can also be combined with AgNPs for improved sensitivity. 20 However, the color change is not due to NP aggregation but to fractal morphological alteration of spherical AgNPs caused by coalescence (Fig.…”

Section: Activatable Colorimetric Systemsmentioning

confidence: 99%