An aptamer based resonance light scattering assay of prostate specific antigen

Chen, Zhanguang; Lei, Yulong; Chen, Xi; Zhen, Wang

doi:10.1016/j.bios.2012.03.041

Cited by 78 publications

(24 citation statements)

References 50 publications

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“…Jolly et al (2015b), instead, reported on an EIS aptamer-based aptasensor that makes use of the anti-fouling properties of sulfo-betaine molecules for the formation of the SAM. Chen et al (2012) instead, proposed an optical method of detection in the range 4 pM to 3 nM with LOD of ~1 pM, based on a resonance light-scattering (RLS) and gold nanoparticles assay where the changes in aptamer conformation upon PSA recognition is exploited.…”

Section: Employing a Sensor Basedmentioning

confidence: 98%

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Formisano

Jolly

Bhalla

et al. 2015

Sensors and Actuators B: Chemical

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The response of an Electrochemical Impedance Spectroscopy (EIS) sensor using DNA aptamers is affected by many factors such as DNA density, charge and conformational changes upon DNA-target binding and buffer conditions. We report here for the first time on the optimisation of an EIS aptamerbased sensor by using Quartz Crystal Microbalance with Dissipation mode (QCM-D). As a case study we employed a DNA aptamer against Prostate Specific Antigen (PSA). PSA detection was achieved by functionalizing the gold sensor surface via thiol chemistry with different ratios of thiolated-DNA aptamer and 6-mercapto-1-hexanol (MCH) used as spacer molecules. PSA binding efficiency can be monitored by measuring QCM-D signals which not only provides information about the mass of PSA bound on the sensor surface but also crucial information about the aptamer conformation and layer hydration.Data generated through QCM-D analysis provided the optimal conditions in terms of aptamer/MCH ratio to maximize the PSA binding. The ratio of 1:200 for DNA aptamer/spacer molecule was found to be optimal for ensuring maximum PSA binding. However, this study showed how a maximum analyte binding does not necessarily correspond to a maximum EIS response, which revealed to be enhanced if a ratio of 1:100 for DNA aptamer/spacer molecule was used. Moreover, by monitoring the QCM-D signal, for the first time a value of the dissociation constant (K d ), equal to 37 nM, was found for the PSA DNA aptamer towards its target. The combination of QCM-D with EIS techniques provide further insight into the effects of mass loading and charge effects that govern the response of an EIS aptasensor, serving as a valuable support for future EIS aptamer-based applications.

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Section: Employing a Sensor Basedmentioning

confidence: 98%

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Formisano

Jolly

Bhalla

et al. 2015

Sensors and Actuators B: Chemical

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“…It has the advantages of simplicity, rapidness and high sensitivity. RLS method has been used for the determination of various target analytes, including inorganic ions [13,14], organic medicines [15][16][17] and biological macromolecules [18][19][20][21], as well as the interactions of medicines with biological macromolecules [22,23]. However, until now, RLS method has hardly been used for the determination of ATP.…”

Section: Introductionmentioning

confidence: 98%

Determination of ATP by resonance light scattering using a binuclear uranyl complex and aptamer modified gold nanoparticles as optical probes

Liao

et al. 2014

Microchim Acta

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We describe a resonance light scattering (RLS) method for the direct detection of adenosine triphosphate (ATP) in aqueous solution without the need for separation. The binuclear uranyl complex bis-uranyl-bis-sulfosalophen (BUBSS) was synthesized by the coordination reaction of two uranyl ions with the ditopic tetradentate ligand bissulfosalophen. In parallel, gold nanoparticles were modified with anti-ATP aptamer (Apt-AuNPs) to serve as RLS probes. The detection scheme is based on the specific reaction of a BUBSS molecule with two ATP molecules to form a linear supramolecule. This, in turn, binds two Apt-AuNPs molecules, and this is accompanied by the aggregation of the Apt-AuNPs and results in a strong RLS signal. A RLS method was developed on this basis that has a linear response to ATP in the 5 to 500 nM concentration range, with a 4.5 nM detection limit under optimal conditions. The method has been successfully applied to the detection of ATP in spiked real samples and gave recoveries between 96.3 and 102.5 %.

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“…The methods are based on the enlargement of particle volume caused by the assemblies or aggregation of the molecules and/or particles. RLS methods have been used for the detection of target analytes based on various assembly and aggregation reactions, such as the self-assembly of ions to form ion association particles [9][10][11][12], the aggregation of nanoparticles [13][14][15][16], the binding of antigens with antibodies to form immune complexes [17], and the combination of small molecules with proteins and DNA [18][19][20][21]. The target analytes include ions [10,11,15], organic and biological molecules [9,[12][13][14], medicines [18][19][20][21], as well as antigens and antibodies [16,17].…”

Section: Introductionmentioning

confidence: 99%

Resonance light scattering detection of uranium based on its reaction with a Schiff base containing tetradentate ligand and phosphate groups to form supramolecular polymer

Liao

et al. 2015

J Radioanal Nucl Chem

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To develop a new resonance light scattering (RLS) method for uranium(VI) detection, we have studied the reaction of uranyl with N,N 0 -bis(pyridoxal phosphate)-o-phenylenediamine (BPPP) that is a Schiff base containing a tetradentate ligand and two phosphate groups. BPPP can chelate uranyl and then self-assemble to form a supramolecular polymer, resulting in a production of strong RLS signal. The RLS method was established based on this fact. The linear range is 0.5-25 ng mL -1 with a detection limit of 0.2 ng mL -1 . The method is simple and has been successfully applied to determine uranium(VI) in environmental water samples.

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An aptamer based resonance light scattering assay of prostate specific antigen

Cited by 78 publications

References 50 publications

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Determination of ATP by resonance light scattering using a binuclear uranyl complex and aptamer modified gold nanoparticles as optical probes

Resonance light scattering detection of uranium based on its reaction with a Schiff base containing tetradentate ligand and phosphate groups to form supramolecular polymer

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