An aptamer-based single particle method for sensitive detection of thrombin using fluorescent quantum dots as labeling probes

Yin, Jinjin; Zhang, Aidi; Dong, Chaoqing; Ren, Jicun

doi:10.1016/j.talanta.2015.05.034

Cited by 15 publications

(4 citation statements)

References 61 publications

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“…Using the nanoprobes containing 22 Nluc, we further investigate the sensitivity of the assay. As low as 2.5 nM thrombin was easily detected (Figure S6D); this detection limit is in line with other reported enzyme-based ELISA assay for thrombin. − …”

Section: Resultssupporting

confidence: 90%

Post-Translational Modification of Bionanoparticles as a Modular Platform for Biosensor Assembly

et al. 2015

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Context driven biosensor assembly with modular targeting and detection moieties is gaining significant attentions. Although protein-based nanoparticles have emerged as an excellent platform for biosensor assembly, current strategies of decorating bionanoparticles with targeting and detection moieties often suffer from unfavorable spacing and orientation as well as bionanoparticle aggregation. Herein, we report a highly modular post-translational modification approach for biosensor assembly based on sortase A-mediated ligation. This approach enables the simultaneous modifications of the Bacillus stearothermophilus E2 nanoparticles with different functional moieties for antibody, enzyme, DNA aptamer, and dye decoration. The resulting easy-purification platform offers a high degree of targeting and detection modularity with signal amplification. This flexibility is demonstrated for the detection of both immobilized antigens and cancer cells.

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Section: Resultssupporting

confidence: 90%

Post-Translational Modification of Bionanoparticles as a Modular Platform for Biosensor Assembly

et al. 2015

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“…Fore xample,asandwich aptamer-based FCS assay utilizing QD-based probes was reported for the detection of thrombin in serum with an LOD of 2.6 nm and aworking range of 5-500 nm. [91] Theg eneral configuration of the FCCS requires the alignment of the two lasers with different wavelengths to the same focal spot, which makes the optical setup more complicated than the conventional single-laser FCS.T he misalignment of the detection volumes can lead to adecrease of apparent cross-correlation. Thes ingle-wavelength excitation FCCS (SW-FCCS) developed by Wohlandsg roup [92] overcame this limitation by the use of two probes,w hich can be excited using the same wavelength, but their emission is separated due to alarge difference of the Stokes shifts.This can be conveniently achieved by the use of QDs [93] or long Stokes shift fluorescent proteins.…”

Section: Fluorescence Correlation Spectroscopymentioning

confidence: 99%

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

et al. 2020

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The ability to detect low concentrations of analytes and in particular low‐abundance biomarkers is of fundamental importance, e.g., for early‐stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single‐molecule bioaffinity assays. While many review articles have highlighted the potentials of single‐molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real‐world applications as one should expect. This Review provides a theoretical background on single‐molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single‐molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.

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“…Beispielsweise wurde ein Aptamer-basierter Sandwich-FCS-Assay unter Verwendung von QDs fürd en Nachweis von Thrombin in Serum mit einem LOD von 2.6 nm und einem Arbeitsbereich von 5-500 nm beschrieben. [91] Der allgemeine Aufbau der FCCS erfordert die gemeinsame Fokussierung beider Laser unterschiedlicher Wellenlänge auf das Detektionsvolumen, wodurch ein komplizierterer optischer Aufbau als beim herkçmmlichen Einzellaser-FCS notwendig ist. Eine Fehlausrichtung der Laser würde die Kreuzkorrelationsbestimmunge rheblich beinträchtigen.…”

Section: Fluoreszenzkorrelationsspektroskopieunclassified

Fortschritte in der optischen Einzelmoleküldetektion: Auf dem Weg zu höchstempfindlichen Bioaffinitätsassays

et al. 2020

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An aptamer-based single particle method for sensitive detection of thrombin using fluorescent quantum dots as labeling probes

Cited by 15 publications

References 61 publications

Post-Translational Modification of Bionanoparticles as a Modular Platform for Biosensor Assembly

Post-Translational Modification of Bionanoparticles as a Modular Platform for Biosensor Assembly

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

Fortschritte in der optischen Einzelmoleküldetektion: Auf dem Weg zu höchstempfindlichen Bioaffinitätsassays

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