An Aptasensing Strategy Using the Phosphatase‐mimic Nanozyme and pH Meter as Signal Readout

Abstract

The demand for point‐of‐care testing (POCT) is growing dramatically, especially for district where health facilities are poorly staffed, poorly skilled and ill‐equipped. As a commercialized portable device, pH meters can be used for detection of various targets, relying on bioactive enzymes. The nanozymes, as the alternative of the natural enzymes, have rarely been used for pH‐metric POCT strategies. Herein, we developed an ultrasensitive pH‐metric sandwich‐type aptasensor based on the CeO2 nanorods (CeO2 NRs) as phosphatase‐mimic and sodium monofluorophosphate (MFP) as catalytic substrate. Under optimal conditions, such strategy yields a detection limit of 1.17 nM with eligible selectivity for detecting thrombin.

A nucleic acid-based magnetic potentiometric aptasensing platform for indirect detection of prostate-specific antigen with catalytic hairpin assembly

“…19,20 Potentiometry (i.e., pH meter) is currently one of the most widely used devices in the world because of its portable size, easy operation, low cost and reliable quantitative results. 21 Potentiometric measurements are often carried out with a two-electrode system including working electrode and reference electrode without external excitement such as current and voltage. 22 23 To the best of our knowledge, a few reports were focused on the potentiometric measurement of cancer biomarkers.…”