An Aptasensor Based on a Flexible Screen-Printed Silver Electrode for the Rapid Detection of Chlorpyrifos

Inam, Aqil; Angeli, Martina Aurora Costa; Douaki, Ali; Shkodra, Bajramshahe; Lugli, Paolo; Petti, Luisa

doi:10.3390/s22072754

Cited by 20 publications

(5 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher stability, longer lifetime, and lower cost are advantages of aptamers over enzymes and antibodies [60]. Aptasensors exhibited high sensitivity to pesticides such as fipronil [62], diazinon [63], chlorpyrifos [64], and acetamiprid [65] in real samples (fruits, vegetables, wastewater). The SELEX process was used to select aptamers capable of distinguishing the insecticide fenitrothion from non-specific targets with LOD of 14 nM [66].…”

Section: Discussionmentioning

confidence: 99%

Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations

Esimbekova

Kalyabina

Kopylova

et al. 2023

IJMS

View full text Add to dashboard Cite

Pesticides can affect the health of individual organisms and the function of the entire ecosystem. Therefore, thorough assessment of the risks associated with the use of pesticides is a high-priority task. An enzyme inhibition-based assay is used in this study as a convenient and quick tool to study the effects of pesticides at the molecular level. The contribution of formulants to toxicological properties of the pesticide formulations has been studied by analyzing effects of 7 active ingredients of pesticides (AIas) and 10 commercial formulations based on them (AIfs) on the function of a wide range of enzyme assay systems differing in complexity (single-, coupled, and three-enzyme assay systems). Results have been compared with the effects of AIas and AIfs on bioluminescence of the luminous bacterium Photobacterium phosphoreum. Mostly, AIfs produce a considerably stronger inhibitory effect on the activity of enzyme assay systems and bioluminescence of the luminous bacterium than AIas, which confirms the contribution of formulants to toxicological properties of the pesticide formulation. Results of the current study demonstrate that “inert” ingredients are not ecotoxicologically safe and can considerably augment the inhibitory effect of pesticide formulations; therefore, their use should be controlled more strictly. Circular dichroism and fluorescence spectra of the enzymes used for assays do not show any changes in the protein structure in the presence of commercial pesticide formulations during the assay procedure. This finding suggests that pesticides produce the inhibitory effect on enzymes through other mechanisms.

show abstract

Section: Discussionmentioning

confidence: 99%

Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations

Esimbekova

Kalyabina

Kopylova

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…The electrochemical signal of the designed aptasensor lost 6.8% after 1 h at RT. It could be due to the degeneration in the performance of the RE after continuous measurements in the same SPEs [54][55][56]. Therefore, each calibration point was measured in three different modified SPEs.…”

Section: Detection Of Aphanizomenon Sp Ulc602mentioning

confidence: 99%

An Electrochemical Aptasensor for the Detection of Freshwater Cyanobacteria

Pham,

Maghsoomi,

Brandl

2024

Biosensors

View full text Add to dashboard Cite

Aphanizomenon is a genus of cyanobacteria that is filamentous and nitrogen-fixing and inhabits aquatic environments. This genus is known as one of the major producers of cyanotoxins that can affect water quality after the bloom period. In this study, an electrochemical aptasensor is demonstrated using a specific aptamer to detect Aphanizomenon sp. ULC602 for the rapid and sensitive detection of this bacterium. The principal operation of the generated aptasensor is based on the conformational change in the aptamer attached to the electrode surface in the presence of the target bacterium, resulting in a decrease in the current peak, which is measured by square-wave voltammetry (SWV). This aptasensor has a limit of detection (LOD) of OD750~0.3, with an extension to OD750~1.2 and a sensitivity of 456.8 μA·OD750−1·cm−2 without interference from other cyanobacteria. This is the first aptasensor studied that provides rapid detection to monitor the spread of this bacterium quickly in a targeted manner.

show abstract

“…One promising area of research is the development of biosensors, which use biological molecules such as enzymes or antibodies to detect specific pesticides (Chang et al, 2021; Inam et al, 2022). Another promising area that has gained attention in recent years is nanoparticle‐mediated pesticide detection.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Sensitivity and Selectivity in Pesticide Detection: A Review of Cutting‐Edge Techniques

Ortiz‐Martínez,

Molina González,

Ramírez García

et al. 2024

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

The primary goal of our review was to systematically explore and compare the state‐of‐the‐art methodologies employed in the detection of pesticides, a critical component of global food safety initiatives. New approach methods in the fields of luminescent nanosensors, chromatography, terahertz spectroscopy, and Raman spectroscopy are discussed as precise, rapid, and versatile strategies for pesticide detection in food items and agroecological samples. Luminescent nanosensors emerge as powerful tools, noted for their portability and unparalleled sensitivity and real‐time monitoring capabilities. Liquid and gas chromatography coupled to spectroscopic detectors, stalwarts in the analytical chemistry field, are lauded for their precision, wide applicability, and validation in diverse regulatory environments. Terahertz spectroscopy offers unique advantages such as noninvasive testing, profound penetration depth, and bulk sample handling. Meanwhile, Raman spectroscopy stands out with its nondestructive nature, its ability to detect even trace amounts of pesticides, and its minimal requirement for sample preparation. While acknowledging the maturity and robustness of these techniques, our review underscores the importance of persistent innovation. These methodologies' significance extends beyond their present functions, highlighting their adaptability to meet ever‐evolving challenges. Environ Toxicol Chem 2024;00:1–17. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

show abstract

An Aptasensor Based on a Flexible Screen-Printed Silver Electrode for the Rapid Detection of Chlorpyrifos

Cited by 20 publications

References 65 publications

Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations

Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations

An Electrochemical Aptasensor for the Detection of Freshwater Cyanobacteria

Enhancing Sensitivity and Selectivity in Pesticide Detection: A Review of Cutting‐Edge Techniques

Contact Info

Product

Resources

About