An aptasensor based on upconversion nanoparticles as LRET donors for the detection of exosomes

Wang, Yonghao; Luo, Dengwang; Fang, Yao; Wu, Wanhao; Wang, Yongjing; Xia, Yaokun; Wu, Fang; Li, Chunyan; Lan, Jianming; Chen, Jinghua

doi:10.1016/j.snb.2019.126900

Cited by 35 publications

(22 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in the presence of the exosome, both fragments, and subsequently nanoparticles, would be brought together, causing the quenching of the fluorescence proportional to the number of exosomes with an LOD of 1.1 × 10 3 particles/µL within approximately 30 min [ 56 ]. A year later, Wang et al were able to achieve a significantly lower LOD of 80 particles/µL with another paper-based aptasensor for the detection of exosomes [ 57 ]. In this experiment, an EpCAM aptamer was divided between an energy donor (UCNPs) and an energy receptor (tetramethylrhodamine (TAMRA)).…”

Section: Upconversion Nanoparticle-based Optical Biosensor For Cirmentioning

confidence: 99%

“…Due to the energy transfer between both particles, TAMRA emits a yellow fluorescence. By quantifying TAMRA’s emissions, it was possible to determine the concentration of EpCAM expressing exosomes [ 57 ]. In another assay by Wang et al, it was possible to achieve an LOD of 6.30 pM for ctDNA by using gold nanocages as energy acceptors [ 58 ].…”

Section: Upconversion Nanoparticle-based Optical Biosensor For Cirmentioning

confidence: 99%

See 1 more Smart Citation

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

2021

View full text Add to dashboard Cite

The effectiveness of cancer treatment strongly depends on the early detection of the disease. Currently, the most common diagnostic method, tissue biopsy, takes time and can be damaging to the patient. Circulating cancer biomarkers such as circulating tumor DNA, micro-RNA (miRNA), tumor proteins, exosomes, and circulating tumor cells have repeatedly demonstrated their viability as targets for minimally invasive cancer detection through liquid biopsies. However, among other things, achieving a great sensitivity of detection is still challenging due to the very low concentration of biomarkers in fluid samples. This review will discuss how the recent advances in nanoparticle-based biosensors are overcoming these practical difficulties. This report will be focusing mainly on optical transduction mechanisms of metal nanoparticles (M-NPs), quantum dots (QDs), and upconversion nanoparticles (UCNPs).

show abstract

Section: Upconversion Nanoparticle-based Optical Biosensor For Cirmentioning

confidence: 99%

Section: Upconversion Nanoparticle-based Optical Biosensor For Cirmentioning

confidence: 99%

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

2021

View full text Add to dashboard Cite

show abstract

“…Wang et al used tetramethyl rhodamine (TAMRA) and UCNPs functionalized with two DNA aptamers to target the epithelial cell adhesion molecule (EpCAM) present on the exosome membrane of some cell lines. 164 After the aptamer-exosome binding, both DNA strands got closer and the distance between the energy donor (UCNPs) and the acceptor (TAMRA) was reduced, promoting the LRET process (Fig. 5).…”

Section: Np-based Biosensors For Ev Analysismentioning

confidence: 99%

Nanoparticle-based biosensors for detection of extracellular vesicles in liquid biopsies

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In a typical LRET-based homogeneous assay, upconversion nanomaterials are first excited by NIR irradiation and then non-radiative energy transfer occurs to the spectrally overlapping acceptors, leading to the luminescence quenching of UCL emission signal. The LRET-based homogeneous assays have greatly improved LOD and use a simple readout process 36,37 . As shown in Figure 4B, progesterone was detected by the sensor platform based on a NIR-to-NIR signal LRET system via competitive immunoassay.…”

Section: Homogeneous Assaymentioning

confidence: 99%

Lanthanide-Doped Upconversion Nanomaterials: Recent Advances and Applications

et al. 2020

View full text Add to dashboard Cite

Lanthanide-doped upconversion nanomaterials are well known for converting near-infrared (NIR) excitation photons into visible, ultraviolet, and NIR emission photons. Excitation of NIR light offers low autofluorescence background and high penetration depth in biological environments due to the reduced light scattering. Consequently, these have attracted considerable interest because their exceptional optical properties have led to applications in diverse areas such as bioassays, biomedical imaging, and forensics; these properties are, namely: large anti-Stokes shifts, sharp emission spectra and long excited-state lifetimes. The nanomaterials can be utilized for a variety of applications including detection of various analytes, bioimaging, therapies, energy conversion, and security. In this review, we briefly describe the upconversion luminescence process, effective synthetic approaches, and recent literature that elucidate the exceptional properties of these materials and its applications.

show abstract

An aptasensor based on upconversion nanoparticles as LRET donors for the detection of exosomes

Cited by 35 publications

References 50 publications

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

Nanoparticle-based biosensors for detection of extracellular vesicles in liquid biopsies

Lanthanide-Doped Upconversion Nanomaterials: Recent Advances and Applications

Contact Info

Product

Resources

About