An Aqueous Extract of Tuberaria lignosa Inhibits Cell Growth, Alters the Cell Cycle Profile, and Induces Apoptosis of NCI-H460 Tumor Cells

Abstract

Tuberaria lignosa (Sweet) Samp. is found in European regions, and has antioxidant properties due to its composition in ascorbic acid and phenolic compounds. Given its traditional use and antioxidant properties, the tumor cell growth inhibitory potential of aqueous extracts from T. lignosa (prepared by infusion and decoction) was investigated in three human tumor cell lines: MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), and HCT-15 (human colorectal adenocarcinoma). Both extracts inhibited the growth of these cell lines; the most potent one being the T. lignosa extract obtained by infusion in the NCI-H460 cells (GI 50 of approximately 50 µg/mL). Further assays were carried out with this extract in NCI-H460 cells. At 100 µg/mL or 150 µg/mL it caused an increase in the percentage of cells in the G0/G1 phase and a decrease of cells in S phase of the cell cycle. Additionally, these concentrations caused an increase in the percentage of apoptotic cells. In agreement, a decrease in total poly (ADP-ribose) polymerase (PARP) and pro-caspase 3 levels was found. In conclusion, the T. lignosa extract obtained by infusion was more potent in NCI-H460 cells, altering the cell cycle progression and inducing apoptosis. This work highlights the importance of T. lignosa as a source of bioactive compounds with tumor cell growth inhibitory potential.

Eucalyptus globulus Labill. decoction extract inhibits the growth of NCI-H460 cells by increasing the p53 levels and altering the cell cycle profile

“…Given this problematic issue, the screening of natural compounds as potential anti-tumor agents has become an interesting approach worldwide. [7][8][9][10][11][12] Most notably, nearly half of the small molecules approved for cancer treatment were either actually natural products or derived from such products. 13 Some of the most relevant anticancer drugs in this category include frequently used agents such as Taxol, vinblastine and camptothecin.…”

Melissa officinalisL. ethanolic extract inhibits the growth of a lung cancer cell line by interfering with the cell cycle and inducing apoptosis

“…In the case of MCF-7 and NCI-H460 cells, the appropriate cellular density was 5.0 × 10 4 cells per well 17,18 and for the AGS cells the cellular density was 7.5 × 10 4 cells per well. 19 Two plates were prepared, one to be analysed at the time of addition of the extracts to cells (T0 plate) and the other to be analysed 48 hours after treating the cells with the extracts.…”

Wild edible plants: Nutritional and toxicological characteristics, retrieval strategies and importance for today's society

“…-Evaluation of bioactivities and health promoting effects: Several WEPs are considered functional foods (Pinela et al, 2016e), i.e., foods that exert beneficial physiological effects that go beyond the basic nutrition capacity. Thus, in parallel with the chemical prospecting studies (which highlight the most promising species in terms of bioactive molecules), the in vitro and in vivo evaluation of antioxidant, anti-inflammatory, antitumor, antimicrobial, antidiabetic, and cardioprotective potential of wild plant-based extracts or isolated compounds should be performed (e.g., the studies of Pereira et al (2016), Guimarães et al (2016) and Nikolić et al (2014) with Tuberaria lignosa (Sweet) Samp., Chamaemelum nobile (L.) All. and Thymus sp.pl., respectively).…”