An Arabidopsis Homeodomain Transcription Factor, <i>OVEREXPRESSOR OF CATIONIC PEROXIDASE 3</i>, Mediates Resistance to Infection by Necrotrophic Pathogens

Coego, Alberto; Ramírez, Vicente; Gil, María José; Flors, Vı́ctor; Mauch‐Mani, Brigitte; Vera, Pablo

doi:10.1105/tpc.105.032375

Cited by 104 publications

(130 citation statements)

References 65 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…3 C and D), an inhibitor of NADPH oxidase in both plant and animal tissues (20,29). The cumulative results indicated that AtPep1 requires H 2 O 2 for the transcription of PDF1.2, consistent with recently proposed pathways for JA͞ethylene signaling (28).…”

Section: Resultssupporting

confidence: 84%

See 1 more Smart Citation

An endogenous peptide signal in Arabidopsis activates components of the innate immune response

Huffaker

Pearce

Ryan

2006

Proc. Natl. Acad. Sci. U.S.A.

546

633

View full text Add to dashboard Cite

endogenous elicitor ͉ plant defense ͉ defensin ͉ hydrogen peroxide S imilarities have been noted among early signaling components of animal and plant innate immune systems, including leucine-rich repeat receptor-mediated recognition of pathogenassociated molecular patterns and͞or elicitors from pathogens and the resulting activation of defense gene transcription involved in early steps of immunity (1-14). Several peptides originating from pathogens can activate the plant innate immune response, including fungal elicitors Pep13, AVR9, and elicitins (1-3), and bacterial elicitors hrpZ, NPP1, flg22, and elf13 (4-7). We report here that a 23-aa peptide, isolated from extracts of Arabidopsis leaves and called AtPep1, exhibits characteristics of an endogenous elicitor of the innate immune response. Endogenous plant peptides that activate genes specifically for defense against pathogens have not been reported previously to our knowledge, although systemin peptides, which are found only in Solanaceae species, activate antiherbivore defense genes. AtPep1 was first identified in soluble extracts of Arabidopsis leaves by its ability, at subnanomolar concentrations, to cause an alkalinization of the medium of suspension cultured cells, a typical response of cell cultures to peptide elicitors (15-19). AtPep1 is derived from the C terminus of a 92-aa precursor protein AtproPep1. The peptide activates the transcription of defensin, a gene extensively studied for its role in innate immunity in Arabidopsis, the production of H 2 O 2 , and the expression of PROPEP1. Constitutive overexpression of PROPEP1 confers resistance against a root pathogen Pythium irregulare. PROPEP1 orthologs are found in numerous important agricultural crop species, including both dicots and monocots, and may provide novel genes for investigating crop productivity. Results and DiscussionAtPep1 was purified to homogeneity ( Fig. 1 A and B) and characterized as a peptide by its molecular mass (Fig. 1C) and amino acid sequence (Fig. 1D), which together indicated that the peptide was not posttranslationally modified. Chemically synthesized AtPep1 was found to be as active as native AtPep1, having a half-maximal activity of Ϸ0.25 nM in the alkalinization assay. Peptides present in an 1% trifluoroacetic acid͞water extract of Arabidopsis tissues were passed through a reverse-phase semipreparative C18 flash chromatography column and separated on a G-25 Sepharose column as described in Materials and Methods. The breakthrough peak was applied to a C18 HPLC column, and 10 l from 2-ml fractions from the column was assayed for alkalinization activity. (B) The peak identified in A as AtPep1 was further purified through two additional chromatography steps and finally purified by narrow-bore HPLC as described in Materials and Methods. Fractions were assayed as in A. The active peak is identified with arrows. (C) Analysis of the biologically active peak by MALDI-MS. (D) The amino acid sequence of the purified peptide, determined by Edman degradation. The daltons calculat...

show abstract

Section: Resultssupporting

confidence: 84%

“…3C). The production of active oxygen species has been associated with the signaling pathway of the innate immune response in plants (28). The induction of both H 2 O 2 and PDF1.2 by AtPep1 was blocked by first supplying the leaves with diphenylene iodominium chloride (DPI) (Fig.…”

Section: Resultsmentioning

confidence: 99%

An endogenous peptide signal in Arabidopsis activates components of the innate immune response

Huffaker

Pearce

Ryan

2006

Proc. Natl. Acad. Sci. U.S.A.

546

633

View full text Add to dashboard Cite

show abstract

“…B. cinerea infection assays were performed using either a spore suspension (2 3 10 5 spores mL 21 ) or colonized agar plugs as indicated in the text. The average lesion size (diameter) and plant decay were measured as described previously (Coego et al, 2005;Veronese et al, 2006).…”

Section: Plant Materials Vigs Experiments and Disease Assaysmentioning

confidence: 99%

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Luo

et al. 2014

Plant Physiology

138

View full text Add to dashboard Cite

Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the JAZ1-DELLA regulatory node is regulated at the transcriptional level in plants under normal growth conditions or during pathogen infection is not known. Here, we demonstrate multiple functions of cotton (Gossypium barbadense) GbWRKY1 in the plant defense response and during development. Although GbWRKY1 expression is induced rapidly by methyl jasmonate and infection by Verticillium dahliae, our results show that GbWRKY1 is a negative regulator of the JA-mediated defense response and plant resistance to the pathogens Botrytis cinerea and V. dahliae. Under normal growth conditions, GbWRKY1-overexpressing lines displayed GA-associated phenotypes, including organ elongation and early flowering, coupled with the down-regulation of the putative targets of DELLA. We show that the GA-related phenotypes of GbWRKY1-overexpressing plants depend on the constitutive expression of Gossypium hirsutum GhJAZ1. We also show that GhJAZ1 can be transactivated by GbWRKY1 through TGAC core sequences, and the adjacent sequences of this binding site are essential for binding specificity and affinity to GbWRKY1, as revealed by dual-luciferase reporter assays and electrophoretic mobility shift assays. In summary, our data suggest that GbWRKY1 is a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression.

show abstract

“…The phenotypes of rst1 correlate with increased levels of JA-biosynthesis and responsive gene expression as well as elevated cutic-ular lipids. Increased resistance of the overexpressor of cationic peroxidase 3 (ocp3) mutant to B. cinerea and P. cucumerina is also COI1-dependent (Coego et al, 2005). PFT1, which encodes the MED25 subunit of Mediator, functions downstream of COI1 in A. brassicicola and B. cinerea defense (Kidd et al, 2009).…”

Section: Jasmonatementioning

confidence: 99%

“…Among these, WRKY33, WRKY70, ZFAR1, ERF1 and ERF104 are required for responses to B. cinerea (Mengiste et al, 2003; Lorenzo et al, 2004;AbuQamar et al, 2006;Zheng et al, 2006; Bethke et al, 2009). Genetic screens identifi ed other important TFs encoding MYB, AS1, and HD-Zip homeodomain proteins (Mengiste et al, 2003;Coego et al, 2005;Nurmberg et al, 2007). The transmission of signals from upstream pathogen recognition factors to the activation of plant defense responses via these TFs is poorly understood especially for responses to important necrotrophic fungi.…”

mentioning

confidence: 99%

Necrotroph Attacks on Plants: Wanton Destruction or Covert Extortion?

Laluk

Mengiste

2010

The Arabidopsis Book

209

168

View full text Add to dashboard Cite

Necrotrophic pathogens cause major pre-and post-harvest diseases in numerous agronomic and horticultural crops infl icting signifi cant economic losses. In contrast to biotrophs, obligate plant parasites that infect and feed on living cells, necrotrophs promote the destruction of host cells to feed on their contents. This difference underpins the divergent pathogenesis strategies and plant immune responses to biotrophic and necrotrophic infections. This chapter focuses on Arabidopsis immunity to necrotrophic pathogens. The strategies of infection, virulence and suppression of host defenses recruited by necrotrophs and the variation in host resistance mechanisms are highlighted. The multiplicity of intraspecifi c virulence factors and species diversity in necrotrophic organisms corresponds to variations in host resistance strategies. Resistance to host-specifi c necrotophs is monogenic whereas defense against broad host necrotrophs is complex, requiring the involvement of many genes and pathways for full resistance. Mechanisms and components of immunity such as the role of plant hormones, secondary metabolites, and pathogenesis-related proteins are presented. We will discuss the current state of knowledge of Arabidopsis immune responses to necrotrophic pathogens, the interactions of these responses with other defense pathways, and contemplate on the directions of future research.: e0136. of 34The Arabidopsis Book Infection by fungal necrotrophs generally involves stages of conidial attachment, germination, host penetration, primary lesion formation, lesion expansion, and tissue maceration followed by sporulation (Prins et al., 2000). Following germination, penetration may be achieved by active mechanisms such as appressoria formation and enzymatic degradation or passively through prior infection or wound sites as well as stomates (Prins et al., 2000). After entry, tissue is decomposed through further cellular dismantling using many of the lytic enzymes employed for initial penetration as well as toxic levels of reactive oxygen species (ROS). Many necrotrophs produce various low-molecular weight phytotoxic metabolites, ranging from host-specifi c to those having adverse effects on many diverse species (van Kan, 2006). Others secrete phytotoxic proteins known to induce necrosis, with the vast majority of broad host necrotrophs producing multiples of both (Pemberton and Salmond, 2004;Gijzen and Nurnberger, 2006;Choquer et al., 2007). Throughout infection, these fungi also actively manipulate host cellular machinery in order to suppress defenses and/or aid in disease progression. Some necrotrophs infl uence host phytohormone levels or employ their own hormone biosynthesis thereby disrupting defense signaling (Prins et al., 2000;Sharon et al., 2004). Others have adapted mechanisms to detoxify host metabolites that interfere with virulence (Morrissey and Osbourn, 1999).Overall, bacterial necrotrophs follow a similar mode of pathogenesis to their fungal counterparts, secreting virulence factors into host tissue to induc...

show abstract

An Arabidopsis Homeodomain Transcription Factor, OVEREXPRESSOR OF CATIONIC PEROXIDASE 3, Mediates Resistance to Infection by Necrotrophic Pathogens

Cited by 104 publications

References 65 publications

An endogenous peptide signal in Arabidopsis activates components of the innate immune response

An endogenous peptide signal in Arabidopsis activates components of the innate immune response

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Necrotroph Attacks on Plants: Wanton Destruction or Covert Extortion?

Contact Info

Product

Resources

About