An artificial diet for continuous rearing of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae)

Abstract

The composition and preparation of an artificial diet for the coffee berry borer, Hypothenemus hampei is described. Ten generations were obtained without any significant change in fecundity. Development at 25°C on this diet was similar to previously reported work using coffee berries. One female reared on this diet lived for 380 days. This diet will permit investigations into the genetics of insecticide resistance, potential biopesticides and other research, requiring a convenient laboratory rearing system. Resume-Le mode de preparation et la composition d'un milieu d'elevage artiflciel pour le scolyte du cafe Hypothenemus hampei est decrit. Jusqu'a present, dix generations ont ete obtenues sans changement significatif de la fecondite. Le cycle de developpement a 25°C est similaire a celui reporte par ailleurs dans la litterature, sur des elevages sur cerises de cafe. Une duree de vie maximale de 380 jours a ete observee sur une femelle elevee sur ce milieu. Ce milieu artiflciel permet de conduire des recherches dans le domaine de la genetique de la resistance aux insecticides, dans la prospection de biopesticides potentiels, ou dans tout autre domaine necessistant un elevage permanent au laboratoire.Mots Cles: Hypothenemus hampei, 61evage, milieu artificiel INTRODUCTIONdifficult to obtain in many regions of the world. The lack of a suitable laboratory rearing method was a Hypothenemus hampei is a major pest of coffee, major constraint to our research into the genetics of feeding exclusively on berries; it does not damage the endosulfan resistance (Brun et al., unpubl. data) and vegetative parts of the plant. Native to Central Africa, the development of methods for the integrated pest it has spread to most coffee producing countries in management of//, hampei. We developed this diet to the world. Significant coffee-growing areas where it provide the large quantities of//, hampei of consistent has not yet been detected include Costa Rica, Hawaii quality and physiological status necessary for this and Papua New Guinea. Its biology and ecology in work. This paper describes an artificial diet comprised different parts of the world have been extensively of readily obtainable ingredients, on which several studied (Waterhouse and Norris, 1989), but little strains of//, hampei have been reared for over 10 attention has been paid to the dietetics and nutrition generations. No detailed information was collected of either larvae or adults. Villacorta (1985) has on the intrinsic rate of increase of the insect on this described an artificial diet for rearing successive diet, since our aim was simply to develop a diet to generations of the coffee berry borer, but the enable other research, formulation includes specialized natural ingredients

Experimental evidence for pseudo-arrhenotoky in Hypothenemus hampei (Coleoptera: Scolytidae)

“…The insects were reared on an artificial medium. Methods for rearing and diet composition are given in Brun et al (1995a).…”

Cry1B and Cry3A are active against Hypothenemus hampei Ferrari (Coleoptera: Scolytidae)

“…Artificial diets have been described for CBB rearing, but the formulation includes components difficult to obtain in many countries (Villacorta, 1989;Brun et al, 1993). We developed a diet (named IBUN) to provide the necessary quantities of H. hampei of consistent quality and physiological status necessary for bioassays in this work (Table 1).…”

Antifungal Agents Against <I>Aspergillus niger</I> for Rearing Rice Leaffolder Larvae (Lepidoptera: Pyralidae) on Artificial Diet

“…Frequently host plant materials are incorporated in the artificial diets of insects (Brun et al 1993;Blossey et al 2000;Wheeler & Zahniser 2001). Incorporation of the natural plant host of insects in an artificial diet may benefit the rearing of insects, probably due to the feeding stimuli or cryptic nutrients in natural plant host (Cohen 2004).…”