An assessment of calibration and performance of the microdialysis system

Urea Clearance: A New Technique Based on Microdialysis to Assess Liver Blood Flow Studied in a Pig Model of Ischemia/Reperfusion

“…In this respect, urea seems promising as it is regarded as a nonvolatile, stable, and nontoxic compound that is often used in microdialysis as an endogenous reference compound [12,13] . It is easily measured and is commonly used as a standard assay among other basic metabolic markers for which a standardized measurement system (for example the CMA 600 system; CMA Microdialysis, Solna, Sweden) has shown favorable characteristics [14] . Transport of urea over the microdialysis membrane is bidirectional; it is known to be evenly distributed throughout the body fluid compartments, and is not metabolized in the liver or known to affect the local metabolism or blood flow in itself.…”

Improving the recovery of S100B protein in cerebral microdialysis: Implications for multimodal monitoring in neurocritical care

“…Our findings lay emphasis on the exercise of caution in the interpretation of single point measures of microdialysis biomarkers and that clinical judgement should be based on a sustained trend over a reasonable period of time (Abrahamsson and Winso, 2005;Hutchinson et al, 2006;Larsen, 2006).…”

“…This is an important question to address in future studies as an overestimation of the lactate concentration and a consequently higher LPR may have implications for future patient managementone example being the Lund concept where the LPR is used to guide medical management of high ICP. Reliability of the CMA 600 had previously been determined by Abrahamsson and Winso (2005) who obtained a peak coefficient of variance of 0.55% for the CMA 600 using plastic vials and a known glucose solution. Hutchinson et al correlated CMA 600 glutamate concentration measurements with those from high performance liquid chromatography (HPLC), finding good correlation with a mean difference of 11.9% (−48 to 20%).…”

“…The relative recovery is a function of several factors, some of which can be manipulated in the experimental or clinical setting (Plock and Kloft, 2005). With the introduction of catheters with larger membrane pore size a better recovery of higher molecular weight (MW) biomarkers seems to be feasible (Benveniste et al, 1989;Abrahamsson and Winso, 2005;Hillman et al, 2005Hillman et al, , 2006Hutchinson et al, 2005;Rosenbloom et al, 2005Rosenbloom et al, , 2006Tisdall and Smith, 2006;Waelgaard et al, 2006). In this paper we describe an in vitro study determining the relative recovery of the protein S100B alongside the conventional biomarkers glucose, lactate and pyruvate by comparing the 20 kDa CMA70 and 100 kDa CMA71 microdialysis catheters (CMA Microdialysis, Solna, Sweden).…”

Validation of LC‐MS/MS method applied to evaluation of free tissue concentrations of vildagliptin in diabetic rats by microdialysis

“…One disadvantage of the technique is that the recovery obtained in the probe is not the same for different drugs and different experimental conditions. Before the microdialysis experiment, a calibration of the probe used in different experimental conditions is needed (Abrahamsson and Winsö, 2005) .…”