PurposeThis paper presents a maturity model for digital transformation effectiveness in laboratories (labs) with education and research purposes.Design/methodology/approachThe model was developed using design science research methodology, expert interviews and case studies.FindingsThe model fulfills three practical goals: (1) to establish comparability of the effectiveness of the digital transformation of labs, (2) to provide lab operators from academia and industry with a guide for (further) transformation and (3) to build initial trust among lab users. In addition, the maturity model contributes to the literature on digital lab transformation by capturing, describing, structuring and evaluating relevant dimensions, items and levels. Model strengths and weaknesses, areas for improvement, international applicability and practical and reusable recommendations are presented as well as the added value in assessing lab functionalities and lab sustainability.Practical implicationsAlthough originally developed as a maturity model driven by lab education, the model is also suitable for the transformation of research labs in manufacturing technology management. Digital labs can efficiently support industry training and research and development activities as well as simulate the development of new processes prior to their implementation.Originality/valueEspecially for these use cases, the authors see application potentials for the use of online labs from an organizational perspective and from the perspective of stakeholders such as industry users and operators with a manufacturing background, who use and develop transformed labs for teaching and research.