An assessment of genetic fidelity of micropropagated plants of Chlorophytum borivilianum using RAPD markers

Abstract

Rapid micropropagation was achieved in Chlorophytum borivilianum Santapau and Fernandes using shoot base as explants. Multiple shoots were induced on Murashige and Skoog's (MS) medium supplemented with 3.0 mg dm -3 6-benzylaminopurine, 0.1 mg dm -3 1-naphthaleneacetic acid, 150 mg dm -3 adenine sulphates and 3 % saccharose. Rooting was readily achieved upon transferring the shoots onto half strength MS medium supplemented with 0.1 mg dm -3 indolebutyric acid and 2 % saccharose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of the micropropagated plants. Thirty one arbitrary decamers were used to amplify genomic DNA from in vitro and in vivo plant material to assess the genetic stability. All RAPD profile analysis from micropropagated plants was genetically similar to mother plants.Additional key words: genetic stability, in vitro, medicinal plant, polymerase chain reaction.

Regeneración de plantas a partir de cultivos de callos de <i>Vitex trifolia</i> (Lamiales: Lamiaceae): una planta medicinal potencial

“…Besides, the combination of cytokinins and auxins sometimes triggered the rate of shoot bud regeneration in various medicinal plants (Sivanesan & Jeong 2007, Samantaray & Maiti 2008, Samantaray et al 2009. Though BAP and NAA played a significant role in shoot bud regeneration, however, addition of adenine sulphate and GA 3 in the culture medium resulted in quick growth of shoot buds within four weeks of culture which is corroborated with the findings of earlier reports (Mohan & Krishnamurthy 1998, Samantaray & Maiti 2010. The percentage of shoot bud regeneration and the frequency of regenerated shoot/culture varied significantly in stem, petiole and leaf explants.…”

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

“…No variations were found and so it can be concluded that regenerated plants were true to type plants. Genetic similarity of micropropagated plants using RAPD markers have been reported in Chlorophytum borivilianum by Samantaray and Maiti (2010), in Capparis decidua by Tyagi et al (2010) and in Curcuma species by Das et al (2010). Xing et al (2010) reported no somaclonal variation in regenerated eggplants raised from leaves and cotyledons and analyzed by flow cytometry, RAPD and SSR markers.…”

Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. & Fernandez

“…However, MS media supplemented with Kn enhanced the in vitro shoot elongation of Asparagus racemosus and Chlorophytum arundinaceum [10,21]. Samantaray and Maiti [22] have used 3 mg L -1 BAP, 0.1 mg L -1 1-naphthaleneacetic acid (NAA), 150 mg L -1 adenine sulfate and 3% saccharose instead of for shoot induction of C. borivilianum.…”