An assessment of shallow and mesophotic reef brachyuran crab assemblages on the south shore of O‘ahu, Hawai‘i

Hurley, Kaleonani; Timmers, Molly A.; Godwin, L. Scott; Copus, Joshua M.; Skillings, Derek; Toonen, Robert J.

doi:10.1007/s00338-015-1382-z

Cited by 36 publications

(29 citation statements)

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“…The affiliation of mesophotic Zoantharia with both shallow and deep (abyssal) realms invokes a novel biogeographic enigma. Previous studies of fishes and crabs have revealed that mesophotic biodiversity is affiliated almost exclusively with the genera and families of the shallow shelf fauna (Kahng et al, 2014;Hurley et al, 2016). Either Abyssoanthidae and other deepsea zoantharians have a greater presence in shallow habitat than previously known, or the mesophotic Zoantharia have a "hybrid" origin that includes some deep-sea fauna.…”

Section: Discussionmentioning

confidence: 98%

“…However, studies on zoantharian at mesophotic and deeper depths are generally lacking, and collection of Zoantharia in mesophotic areas lags behind other benthic organisms such as sponges, stony corals, and octocorals. Thus, it is not known whether such depths harbor species affiliated with shallow (0-30 m) or deep (>200 m) communities, a mixture of both (=ecotone; region of transition between two biological communities), or unique depth-specific biodiversity as observed in brachyuran crabs (Hurley et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Exploring the Biodiversity of Understudied Benthic Taxa at Mesophotic and Deeper Depths: Examples From the Order Zoantharia (Anthozoa: Hexacorallia)

et al. 2019

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Research on so-called "minor taxa" at mesophotic depths has lagged behind that of more commonly researched groups such as fish or hard corals. Exemplar taxa include species of the order Zoantharia, benthic colonial anemones that are cosmopolitan in distribution but understudied in many ecoregion and ecosystems. In this study, we examine the results of rare mesophotic to deep sea surveys (one rebreather, one remotely operated submersible (ROV) + net survey, two ROV surveys) from Japan and Israel. We examined the collected images and video data to provide a first estimate of Zoantharia diversity at mesophotic depths. Zoantharians were observed in all surveys, indicating their ubiquitous presence in mesophotic and deeper ecosystems. Additionally, specimens (n = 12) acquired via these surveys were phylogenetically analyzed with three DNA markers. Phylogenetic results showed the presence of undescribed species based on the uniqueness of acquired DNA sequences, including one specimen belonging to the family Abyssoanthidae, previously only reported from depths below 2000 m. Other specimens belong to groups that inhabit shallower and deeper depths (Antipathozoanthus, Epizoanthus, and Parazoanthidae). These results depart from surveys of mesophotic coral ecosystem fishes and crustaceans, which indicate affiliations almost exclusively with shallow genera and families. We hypothesize that mesophotic depths are an ecotone for zoantharian diversity, with links to both deeper and shallower ecosystem diversity. Future surveys in mesophotic depths should incorporate phylogenetic methods to better catalog so-called "minor taxa" (not only including Zoantharia) and enhance the scientific foundation for conserving the biodiversity of these threatened ecosystems.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Exploring the Biodiversity of Understudied Benthic Taxa at Mesophotic and Deeper Depths: Examples From the Order Zoantharia (Anthozoa: Hexacorallia)

et al. 2019

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“…Sequencing, however, typically requires access to a well-equipped molecular laboratory 84 (Glenn, 2011;Quail et al, 2012), and Sanger costs remain high at US$18 per sample (Meier 85 et al, 2016), making ARMS sequencing an expensive endeavour. Consequently, researchers 86 have resorted to using imaging techniques and morphological examination of the ARMS 87 plates as an alternative to assessing biodiversity (Hurley et al, 2016;David et al, 2019), 88 despite morphology-based approaches being less cost-effective (Hayes et al, 2005). Sanger 89 sequencing costs may also be the reason there is a decreasing representation of >2-mm size 90 fractions ( Supplementary Table 1).…”

Section: Introduction 49mentioning

confidence: 99%

MinION-in-ARMS: Nanopore Sequencing To Expedite Barcoding Of Specimen-Rich Macrofaunal Samples From Autonomous Reef Monitoring Structures

Chang

Bauman

et al. 2020

Preprint

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24Autonomous Reef Monitoring Structure (ARMS) are standardised devices for sampling 25 biodiversity in complex marine benthic habitats such as coral reefs. When coupled with DNA 26 sequencing, these devices greatly expand our ability to document marine biodiversity. 27Unfortunately, the existing workflow for processing macrofaunal samples (>2-mm) in the 28 ARMS pipeline-which involves Sanger sequencing-is expensive, laborious, and thus 29 prohibitive for ARMS researchers. Here, we propose a faster, more cost-effective alternative 30 by demonstrating a successful application of the MinION-based barcoding approach on the 31 >2mm-size fraction of ARMS samples. All data were available within 3.5-4 h, and 32 sequencing costs relatively low at approximately US$3 per MinION barcode. We sequenced 33 the 313-bp fragment of the cytochrome c oxidase subunit I (COI) for 725 samples on both 34 MinION and Illumina platforms, and retrieved 507-584 overlapping barcodes. MinION 35 barcodes were highly accurate (~99.9%) when compared with Illumina reference barcodes. 36 Molecular operational taxonomic units inferred between MinION and Illumina barcodes were 37 consistently stable, and match ratios demonstrated highly congruent clustering patterns 38 (≥0.96). Our method would make ARMS more accessible to researchers, and greatly expedite 39 the processing of macrofaunal samples; it can also be easily applied to other small-to-40 moderate DNA barcoding projects (<10,000 specimens) for rapid species identification and 41 discovery. 42 43 44 45 46 47 48

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“…While this suggests that there is some connectivity between SWRs and the upper MCEs (i.e., between 30 and 60 m), this does not extend significantly to either shallower (13 m) or deeper (90 m) depths. Hurley et al (2016) further suggest that the 30-60-m assemblages represent either a transition between SWRs and lower MCEs or a unique intermediate assemblage.…”

Section: Vertical Connectivity and The Drrhmentioning

confidence: 83%

“…Studies on mesophotic taxa of limited vagility are rare, and the study of Hurley et al (2016) on the species assemblages of brachyuran crabs from 12 to 90 m in Hawaii helps to fill an important gap in evaluating connectivity and the DRRH. Using specimens obtained from 2-yr deployments of collectors, they found the greatest separation among assemblages between 12 and 30 m, with the latter most closely related to the assemblage at 60 m. Sixty-one percent of observed species occurred at 12 m, and 40 % occurred only at this depth.…”

Section: Vertical Connectivity and The Drrhmentioning

confidence: 99%

Theme section on mesophotic coral ecosystems: advances in knowledge and future perspectives

et al. 2016

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The Second International Mesophotic Coral Ecosystems (MCEs) workshop was held in Eilat, Israel, October 26-31, 2014. Here we provide an account of: (1) advances in our knowledge of MCE ecology, including the central question of the potential vertical connectivity between MCEs and shallow-water reefs (SWRs), and that of the validity of the deep-reef refugia hypothesis (DRRH); (2) the contribution of the 2014 MCE workshop to the central question presented in (1), as well as its contribution to novel MCE studies on corals, sponges, fish, and crabs; and (3) gaps, priorities, and recommendations for future research stemming from the workshop. Despite their close proximity to well-studied SWRs, and the growing evidence of their importance, our scientific knowledge of MCEs is still in its infancy. During the last five years, we have witnessed an ever-increasing scientific interest in MCEs, expressed in the exponential increase in the number of publications studying this unique environment. The emerging consensus is that lower MCE benthic assemblages represent unique communities, either of separate species or genetically distinct individuals within species, and any significant support for the DRRH will be limited to upper MCEs. Determining the health and stability of MCEs, their biodiversity, and the degree of genetic connectivity among SWRs and MCEs, will ultimately indicate the ability of MCEs to contribute to the resilience of SWRs and help to guide future management and conservation strategies. MCEs deserve therefore management consideration in their own right. With the technological advancements taking place in recent years that facilitate access to MCEs, the prospects for exciting and innovative discoveries resulting from MCE research, spanning a wide variety of fields, are immense.

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An assessment of shallow and mesophotic reef brachyuran crab assemblages on the south shore of O‘ahu, Hawai‘i

Cited by 36 publications

References 50 publications

Exploring the Biodiversity of Understudied Benthic Taxa at Mesophotic and Deeper Depths: Examples From the Order Zoantharia (Anthozoa: Hexacorallia)

Exploring the Biodiversity of Understudied Benthic Taxa at Mesophotic and Deeper Depths: Examples From the Order Zoantharia (Anthozoa: Hexacorallia)

MinION-in-ARMS: Nanopore Sequencing To Expedite Barcoding Of Specimen-Rich Macrofaunal Samples From Autonomous Reef Monitoring Structures

Theme section on mesophotic coral ecosystems: advances in knowledge and future perspectives

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