An Assessment of the Serum Activity of ADH and ALDH in Patients with Primary Biliary Cholangitis

Abstract

Primary biliary cholangitis (PBC; previously known as primary biliary cirrhosis) is a chronic inflammation-induced cholestatic process in the liver. Antimitochondrial antibodies (AMAs) are observed in around 90% of patients, which suggests that PBC is an autoimmune disease. Alcohol dehydrogenase (ADH), ADH isoenzymes and aldehyde dehydrogenase (ALDH) are localized in the liver, and they are useful markers of liver dysfunction. In this study, the activity of total ADH, ADH isoenzymes and ALDH was evaluated in the blood serum of patients with PBC. The experimental group comprised 50 PBC patients, both male and female, aged 28–67. The control group consisted of 50 healthy subjects, both male and female, aged 25–65. The serum activity of class I ADH, class II ADH and ALDH was measured by spectrofluorophotometry, whereas total ADH and class III ADH activity was determined by photometry methods. The activity of class I ADH and total ADH was significantly higher in the experimental group than in the control group (p < 0.001). An increase in class I ADH and total ADH activity indicates that the isoenzyme class I ADH is released by compromised liver cells and can be useful diagnostic markers of PBC.

Preparation of Alcohol Dehydrogenase–Zinc Phosphate Hybrid Nanoflowers through Biomimetic Mineralization and Its Application in the Inhibitor Screening

“…ADH catalyzes the oxidation of primary and secondary alcohols to produce corresponding aldehydes and ketones [ 6 ], in which the cofactor nicotinamide adenine dinucleotide (NAD + ) is reduced to NADH [ 7 ]. Studies have reported that in alcoholism or cirrhosis, ADH activity is significantly increased in serum [ 8 , 9 ]. The initial activity of ADH is positively correlated with liver damage-related indicators [ 10 ].…”