An association of anti-elastin IgA antibodies with development of retinopathy in diabetic children

Nicoloff, G.; Baydanoff, Stephan; Stanimirova, N.; Petrova, Ch.; Christova, P.

doi:10.1016/s0306-3623(01)00095-7

Cited by 25 publications

(29 citation statements)

References 18 publications

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“…Levels of hemoglobin A 1c and albumin excretion rate were measured as described in our previous report. 17 Neuropathy was diagnosed by clinical examination and sensory tests (skin sensation, vibration sense, light touch) supported by an electrophysiologic test (electromyography).…”

Section: Methodsmentioning

confidence: 99%

Abnormal Levels of Serum Antielastin Antibodies in Children with Diabetes Mellitus Type 1

Nicoloff

Weiss²,

Iotova³

et al. 2006

Journal of Investigative Medicine

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Antibodies to alpha-elastin (elastin breakdown product) and elastin sequences devoid of cross-linked regions (linear elastin) are found in the serum of all human subjects and correlate with their respective serum peptide levels. The aim of this study was to determine if the serum level of antielastin antibodies (AEAbs) differs between type 1 diabetic children and nondiabetic children. Enzyme-linked immunosorbent assay was used to measure the levels of immunoglobulin (Ig)G and IgM AEAbs in the sera of 45 diabetic children (mean age 12.8 +/- 3.2 years, diabetes duration 5.3 +/- 3.6 years). Twenty-two children presented with vascular complications (group 1), whereas 23 displayed no vascular complications (group 2). The controls were 18 healthy children (mean age 11.9 +/- 2.3 years). Diabetic patients showed statistically significant higher levels of IgM alpha-AEAbs (0.82 +/- 0.26 vs 0.61 +/- 0.14, p = .0013) than the control group. In group 1, alpha-AEAbs showed statistically significant higher level than controls: IgG (0.86 +/- 0.42 vs 0.59 +/- 0.12; p = .0109) and IgM (0.88 +/- 0.24 vs 0.61 +/- 0.14; p = .0001). IgM antilinear elastin antibodies (ALEAbs) in group 1 were significantly lower than in controls (0.462 +/- 0.191 vs 0.652 +/- 0.127; p = .0009). IgG alpha-AEAbs showed correlation with microalbuminuria (r = -.26; p = .05) and IgM ALEAbs correlated with microalbuminuria (r = -.32; p = .035). IgG alpha-AEAbs correlated with neuropathy (r = -.32; p = .035). Group 1 patients displayed a correlation between IgG ALEAbs and retinopathy (r = -.48; p = .023) and IgM ALEAbs and microalbuminuria (r = .52; p = .014). Levels of AEAbs and ALEAbs can serve as immunologic markers of the extent of elastin degradation. These markers may provide a tool to study elastin metabolism and a potential clinical role for AEAbs in the pathogenesis and development of vascular complications in diabetic children.

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Section: Methodsmentioning

confidence: 99%

Abnormal Levels of Serum Antielastin Antibodies in Children with Diabetes Mellitus Type 1

Nicoloff

Weiss²,

Iotova³

et al. 2006

Journal of Investigative Medicine

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“…In our previous study in diabetic patients, we found that the increase in AEAbs IgM and IgG suggests an initial pathological autoimmunization to human elastin while the elevation of AEAbs IgA levels -a late accelerated elastin degradation and development of vascular disease [2]. In these studies, however, it was not possible to specify which of AEAbs are components of immune complexes and which are free in the circulation.…”

Section: Discussionmentioning

confidence: 90%

“…Normally AEAbs IgG as well as IgA, IgM, IgD can be found in very low levels in the human body [14]. AEAbs from different immunoglobulin classes are present also in pathological conditions, but at much higher levels [2,[14][15][16][17][18]. α-elastin is an oxaloacetic soluble product of the insoluble "native" elastin.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies in patients with type 1 diabetes mellitus found an increased degradation of elastin -the major protein of elastic fibers. As a result, soluble elastin-degradation peptides (EDP) are released in the circulation and act as a pathological stimulus for the formation of anti-elastin antibodies (AEAbs) [2]. In type 2 diabetes mellitus, different types of autoantibodies have been detected: insulin autoantibodies, GAD-antibodies (glutamic acid decarboxylase antibodies), autoantibodies to tyrosine phosphatase IA2, islet antibodies [3], anti-elastin antibodies [2] and collagen type IV autoantibodies.…”

Section: Introductionmentioning

confidence: 99%

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Increased elastin turnover in diabetic patients with arterial hypertension

Николов¹,

Tsinlikov²,

Nicoloff³

et al. 2013

cejoi

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0001). Group 1 (patients with microvascular complications) showed a significant increase in free AeAbs igG in comparison with controls: 0.428 (0.343-0.591) vs. 0.240 (0.212-0.305), respectively (kW = 20.14; p < 0.0001). Group 2 also shows higher levels of free AeAbs igG than the control group: 0.398 (0.312-0.467) vs. 0.240 (0.212-0.305), respectively (kW = 8.88; p = 0.003). patients with microvascular complications showed the highest levels of free AeAbs igG. There were no significant differences between group 1 and group 2.Conclusions: our results show the association between the activity of elastin turnover and microvascular lesions in diabetic patients with arterial hypertension. We suggest that free AeAbs igG mark a later "secondary" step of the autoimmunization to elastin.

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“…We used a method for elastin-degradation peptides (EDP) determination developed in "General Biology" Section (4,15). Every well of the used polystearene plates is flushed with antihuman monoclonal antibodies against elastin / SIGMA, USA/ (10µg/ml) with 0.05 M carbonate buffer (pH-9.6) and was incubated for three hours up to 37° C and twenty four hours at 4° C.…”

Section: Immunologic Methods For Elastin and Collagen Testingmentioning

confidence: 99%

Immunological Changes in Elastin Degradation Indices in Prehypertensive Patients

Tisheva¹

2005

Biotechnology & Biotechnological Equipment

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An association of anti-elastin IgA antibodies with development of retinopathy in diabetic children

Cited by 25 publications

References 18 publications

Abnormal Levels of Serum Antielastin Antibodies in Children with Diabetes Mellitus Type 1

Abnormal Levels of Serum Antielastin Antibodies in Children with Diabetes Mellitus Type 1

Increased elastin turnover in diabetic patients with arterial hypertension

Immunological Changes in Elastin Degradation Indices in Prehypertensive Patients

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