An Ataxia Test Battery Not Requiring the Use of Rails.

Fregly, Alfred R.; Graybiel, Ashton

doi:10.1037/e417972004-001

Cited by 42 publications

(50 citation statements)

References 1 publication

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“…Following immunohistochemical methods (Fregly and Graybiel, 1968;Nishiwaki et al, 2000), longissimus dorsi tissue samples were fixed in 4% paraformaldehyde, embedded in paraffin according to routine histological procedures, sectioned at a thickness of 4 μm, and dehydrated through an alcohol series. Samples were then incubated with monoclonal antibody "bs-7549R" (1:100), specific to the GAS6 protein, at 4°C overnight, and washed 3 times with phosphate-buffered saline (PBS) for 5 min.…”

Section: Immunohistochemical Experimentsmentioning

confidence: 99%

Characterization of porcine GAS6 cDNA gene and its expression analysis in weaned piglets

Xu¹,

Jiang²,

He³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The growth arrest-specific 6 gene (GAS6) is a member of the family of plasma vitamin K-dependent proteins, which are able to bind to phospholipids using an N-terminal gamma-carboxyglutamic acid domain. A recent report has demonstrated that the GAS6 gene can promote fat deposition and is associated with an increased number of fat cells in mice. In order to investigate whether GAS6 expression is associated with meat quality in pigs, a 2382-bp cDNA sequence of the porcine GAS6 gene (GenBank accession No. KC526197) was first obtained using rapid amplification of cDNA ends from porcine longissimus dorsi tissue. One A/G single nucleotide polymorphism anchored in exon 12 was genotyped using the marker PCR-RFLP-BglI, and the G allele was dominant in the pig breeds tested. Quantitative real-time polymerase chain reaction showed that the porcine GAS6 gene was expressed in all tissues examined in weaned male Shaziling (SZL) and Yorkshire (YS) weanling piglets, and mRNA expression of GAS6 in the longissimus dorsi tissue of SZL piglets was significantly higher than that in YS piglets (P < 0.05). The GAS6 protein was likely to be a membrane protein and was detected in longissimus dorsi tissue from SZL and YS piglets using immunohistochemistry, with an 17661 Expression of the GAS6 gene in weaned pigs ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17660-17672 (2015) abundant protein expression index (P > 0.05). The results imply that the GAS6 gene can be considered a potential candidate for meat quality trait selection and fat deposition in pigs.

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Section: Immunohistochemical Experimentsmentioning

confidence: 99%

Characterization of porcine GAS6 cDNA gene and its expression analysis in weaned piglets

Xu¹,

Jiang²,

He³

et al. 2015

Genet. Mol. Res.

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show abstract

“…For immunohistochemical experiments (Fregly and Graybiel, 1968;Nishiwaki et al, 2000), the longissimus dorsi muscle tissue was embedded in paraffin after fixing in 4% paraformaldehyde. Sections (4 μm thick), were dehydrated by passing through an alcohol series.…”

Section: Immunohistochemical Experimentsmentioning

confidence: 99%

Molecular characterization, chromosome mapping, and expression profile of porcine CDC16

Xu¹,

Jiang²,

Ma³

2015

Genet. Mol. Res.

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ABSTRACT. Cell division cycle16 (CDC16) is a core component among the eight protein subunits of the anaphase-promoting complex (APC). APC is a cyclin degradation system that governs the exit of cells from mitosis. Not much information is available for CDC16 in pig. In this study, a 2284-bp cDNA of porcine CDC16 was obtained by rapid amplification of cDNA ends (RACE). Porcine CDC16 was assigned to SSC11 q11-17, and was determined to be significantly linked with SW1452 by using somatic cell hybrid panel and radiation hybrid panel. One novel A/G SNP anchored in intron 7 of the gene was genotyped by restriction enzyme polymerase chain reaction (PCR)-restriction fragment length polymorphism-Csp6I. In five pig breeds, Shaziling, Taoyuan, Duroc, Landrace, and Yorkshire, the A allele frequency was dominant. Quantitative PCR revealed that porcine CDC16 was expressed in ten selected tissues of 25-day-old Shaziling and Yorkshire piglets, and that the mRNA expression of CDC16 in longissimus dorsi muscle of Shaziling was higher than that of Yorkshire. Expression levels of CDC16 were highest in longissimus dorsi muscle followed by that in pancreas. CDC16 protein was detected in longissimus dorsi muscle of 25-day-old Shaziling and Yorkshire piglets by immunohistochemistry with abundant protein expression index (P > 0.05). This study provides an insight into the role of porcine CDC16 in the formation of meat.

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“…Additional information concerning the administration and scoring of the test battery appears in the article by Pregly and Graybiel ( 8 ) .…”

Section: Walk On Floor Eyes Closed (Wofec)mentioning

confidence: 99%

“…Measures of postural equilibrium were obtained by using the quantitative ataxia test battery developed by Fregly and Graybiel ( 8 ) . The tests were conducted on a hard floor while the subject assumed an erect or .…”

Section: Subjects On the Basis Of Their Responses ( I ) During Intermentioning

confidence: 99%

Effects of congener and noncongener alcoholic beverages on a clinical ataxia test battery.

Schroeder¹,

Collins²

1979

PsycEXTRA Dataset

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An Ataxia Test Battery Not Requiring the Use of Rails.

Cited by 42 publications

References 1 publication

Characterization of porcine GAS6 cDNA gene and its expression analysis in weaned piglets

Characterization of porcine GAS6 cDNA gene and its expression analysis in weaned piglets

Molecular characterization, chromosome mapping, and expression profile of porcine CDC16

Effects of congener and noncongener alcoholic beverages on a clinical ataxia test battery.

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